Abstract
An elicitor preparation obtained from Phytophthora parasitica var. nicotianae, a pathogen of tobacco, induced an accumulation of proteinase inhibitors and a stimulation of ethylene synthesis in a tobacco (Nicotiana tabacum) cell suspension culture. About 30 micrograms per milliliter of elicitor were necessary for maximal induction of proteinase inhibitor accumulation, and the response was detectable after 12 hours of incubation with elicitor. Accumulation of proteinase inhibitors required de novo protein synthesis, since cycloheximide completely inhibited its elicitation, and actinomycin D inhibited it partially. One of the inhibitors was purified by a procedure that included heating, (NH(4))(2)SO(4) precipitation, ion-exchange chromatography, and affinity chromatography. The purified inhibitor was shown to be a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of about 10,500. It inhibited trypsin but not chymotrypsin.
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Claims 10
Claim · model-written summary
Pathogen elicitor induced proteinase inhibitor accumulation in tobacco suspension cells.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- An elicitor preparation obtained from Phytophthora parasitica var. nicotianaee0
- Interactor
- Phytophthora parasitica var. nicotianae
Phytophthora nicotianaee0pathogen - Outcome
- accumulation of proteinase inhibitorse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An elicitor preparation obtained from Phytophthora parasitica var. nicotianae, a pathogen of tobacco, induced an accumulation of proteinase inhibitors and a stimulation of ethylene synthesis in a tobacco (Nicotiana tabacum) cell suspension culture.”
Claim · model-written summary
Pathogen elicitor stimulated ethylene synthesis in tobacco suspension cells.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- An elicitor preparation obtained from Phytophthora parasitica var. nicotianaee0
- Interactor
- Phytophthora parasitica var. nicotianaePhytophthora nicotianaee0pathogen
- Outcome
- ethylene synthesise0
- Direction
- stimulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An elicitor preparation obtained from Phytophthora parasitica var. nicotianae, a pathogen of tobacco, induced an accumulation of proteinase inhibitors and a stimulation of ethylene synthesis in a tobacco (Nicotiana tabacum) cell suspension culture.”
Claim · model-written summary
About 30 micrograms per milliliter of elicitor maximized inhibitor induction.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- 30 micrograms per milliliter of elicitore0
- Interactor
- Phytophthora parasitica var. nicotianaePhytophthora nicotianaee2pathogen
- Outcome
- proteinase inhibitor accumulatione0
- Direction
- maximal inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“About 30 micrograms per milliliter of elicitor were necessary for maximal induction of proteinase inhibitor accumulation”
e1“tobacco (Nicotiana tabacum)”
e2“Phytophthora parasitica var. nicotianae”
Claim · model-written summary
The inhibitor response was detectable after 12 hours of elicitor incubation.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- response was detectablee0
- Qualifier
- after 12 hours of incubation with elicitore0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the response was detectable after 12 hours of incubation with elicitor”
e1“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Cycloheximide completely blocked elicited inhibitor accumulation.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- cycloheximidee0
- Outcome
- Accumulation of proteinase inhibitorse1
- Direction
- completely inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cycloheximide completely inhibited its elicitation”
e1“Accumulation of proteinase inhibitors required de novo protein synthesis”
e2“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Actinomycin D partially blocked elicited inhibitor accumulation.
- Taxon
- tobaccoNicotiana tabacume2
- Treatment
- actinomycin De0
- Outcome
- Accumulation of proteinase inhibitorse1
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“actinomycin D inhibited it partially”
e1“Accumulation of proteinase inhibitors”
e2“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Inhibitor accumulation required new protein synthesis.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- Accumulation of proteinase inhibitorse0
- Qualifier
- de novo protein synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of proteinase inhibitors required de novo protein synthesis”
e1“tobacco (Nicotiana tabacum)”
Claim · model-written summary
A purified inhibitor appeared as a single electrophoretic band of molecular weight about 10,500.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- single bande0
- Value
- about 10,500e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified inhibitor was shown to be a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight of about 10,500”
e1“tobacco (Nicotiana tabacum)”
Claim · model-written summary
The purified inhibitor inhibited trypsin.
- Taxon
- tobaccoNicotiana tabacume2
- Gene
- trypsine0
- Outcome
- inhibited trypsine0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It inhibited trypsin but not chymotrypsin”
e1“The purified inhibitor”
e2“tobacco (Nicotiana tabacum)”
Claim · model-written summary
The purified inhibitor did not inhibit chymotrypsin.
- Taxon
- tobaccoNicotiana tabacume2
- Gene
- chymotrypsine0
- Outcome
- not chymotrypsine0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It inhibited trypsin but not chymotrypsin”
e1“The purified inhibitor”
e2“tobacco (Nicotiana tabacum)”
The same questions in other papers
cycloheximide · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
10 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
8 papers more in this group
See all 12 papers →Phytophthora nicotianae · molecular: metabolites · Nicotiana tabacum (tobacco)Results agree
1 paper in the same direction as this paper
- positive
actinomycin D · molecular: metabolites · Nicotiana tabacum (tobacco)Results agree
3 papers in the same direction as this paper
- no effect
- negative
- negative
resistance inducer · physiology: hormones · Nicotiana tabacum (tobacco)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
resistance inducer · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →resistance inducer · Phytophthora nicotianae · Nicotiana tabacum (tobacco)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.