Abstract
alpha-1,4-Linked oligogalacturonides (OGs) inhibit auxin-regulated transcriptional activation of a rolB-beta-glucuronidase (GUS) gene fusion in tobacco (Nicotiana tabacum) leaf explants (D. Bellincampi, M. Cardarelli, D. Zaghi, G. Serino, G. Salvi, C. Gatz, F. Cervone, M. M. Altamura, P. Costantino, G. De Lorenzo [1996] Plant Cell 8: 477-487). In this paper we show that inhibition by OGs is very rapid, with a short lag time, and takes place even after rolB promoter activation has initiated. OGs also induce a transient and catalase-sensitive accumulation of H(2)O(2) in the leaf explant culture medium. OGs with a degree of polymerization from 12 to 15 are required for both the inhibition of the auxin-induced rolB-driven accumulation of GUS and the induction of H(2)O(2) accumulation(.) However, OG concentration for half-maximal induction of H(2)O(2) accumulation is approximately 3-fold higher than that for half-maximal inhibition of rolB promoter activity. The inhibition of rolB promoter activity is not influenced by the addition of catalase or superoxide dismutase, suggesting that H(2)O(2) and superoxide are not involved in this effect. A fungal oligo-beta-glucan elicitor induces extracellular H(2)O(2) accumulation at comparable or higher levels than those observed with OGs, but does not prevent the auxin-induced accumulation of GUS. We conclude that H(2)O(2) produced upon treatment with OGs is not involved in the inhibition of the auxin-induced expression of the rolB gene.
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Claims 9
Claim · model-written summary
OG inhibition of rolB promoter activity was rapid and occurred after promoter activation began.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Treatment
- OGse0
- Gene
- rolBe0
- Outcome
- inhibition by OGse0
- Qualifier
- even after rolB promoter activation has initiatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibition by OGs is very rapid, with a short lag time, and takes place even after rolB promoter activation has initiated.”
t0“Tobacco”
Claim · model-written summary
OGs induced transient catalase-sensitive H(2)O(2) accumulation in explant medium.
- Taxon
- TobaccoNicotiana tabacumt0
- Organ / stage
- leaf explante0
- Treatment
- OGse0
- Outcome
- accumulation of H(2)O(2)e0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OGs also induce a transient and catalase-sensitive accumulation of H(2)O(2) in the leaf explant culture medium.”
t0“Tobacco”
Claim · model-written summary
OGs of polymerization degree 12 to 15 inhibited auxin-induced rolB-driven GUS accumulation.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- OGs with a degree of polymerization from 12 to 15e0
- Gene
- rolBe0
- Outcome
- auxin-induced rolB-driven accumulation of GUSe0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OGs with a degree of polymerization from 12 to 15 are required for both the inhibition of the auxin-induced rolB-driven accumulation of GUS and the induction of H(2)O(2) accumulation(.)”
t0“Tobacco”
Claim · model-written summary
OGs of polymerization degree 12 to 15 induced H(2)O(2) accumulation.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- OGs with a degree of polymerization from 12 to 15e0
- Outcome
- H(2)O(2) accumulatione0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OGs with a degree of polymerization from 12 to 15 are required for both the inhibition of the auxin-induced rolB-driven accumulation of GUS and the induction of H(2)O(2) accumulation(.)”
t0“Tobacco”
Claim · model-written summary
The OG concentration for half-maximal H(2)O(2) induction was roughly threefold that for half-maximal rolB inhibition.
- Taxon
- TobaccoNicotiana tabacumt0
- Outcome
- OG concentration for half-maximal induction of H(2)O(2) accumulatione0
- Direction
- highere0
- Value
- 3-folde0
- Comparator
- half-maximal inhibition of rolB promoter activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OG concentration for half-maximal induction of H(2)O(2) accumulation is approximately 3-fold higher than that for half-maximal inhibition of rolB promoter activity.”
t0“Tobacco”
Claim · model-written summary
Catalase and superoxide dismutase did not influence OG inhibition of rolB promoter activity.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- addition of catalase or superoxide dismutasee0
- Gene
- rolBe0
- Outcome
- inhibition of rolB promoter activitye0
- Direction
- not influencede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibition of rolB promoter activity is not influenced by the addition of catalase or superoxide dismutase”
t0“Tobacco”
Claim · model-written summary
A fungal oligo-beta-glucan elicitor induced extracellular H(2)O(2) at levels comparable to or above OGs.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- A fungal oligo-beta-glucan elicitore0
- Outcome
- extracellular H(2)O(2) accumulatione0
- Direction
- comparable or highere0
- Comparator
- OGse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A fungal oligo-beta-glucan elicitor induces extracellular H(2)O(2) accumulation at comparable or higher levels than those observed with OGs”
t0“Tobacco”
Claim · model-written summary
The fungal oligo-beta-glucan elicitor did not prevent auxin-induced GUS accumulation.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- A fungal oligo-beta-glucan elicitore0
- Outcome
- auxin-induced accumulation of GUSe0
- Direction
- does not prevente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A fungal oligo-beta-glucan elicitor induces extracellular H(2)O(2) accumulation at comparable or higher levels than those observed with OGs, but does not prevent the auxin-induced accumulation of GUS.”
t0“Tobacco”
Claim · model-written summary
The authors concluded OG-induced H(2)O(2) is not involved in rolB expression inhibition.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- OGse0
- Gene
- rolBe0
- Compound
- H(2)O(2)e0
- Outcome
- inhibition of the auxin-induced expression of the rolB genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H(2)O(2) produced upon treatment with OGs is not involved in the inhibition of the auxin-induced expression of the rolB gene.”
t0“Tobacco”
The same questions in other papers
resistance inducer · physiology: hormones · Nicotiana tabacum (tobacco)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.