Abstract
The effects of fungal elicitor on inositol phospholipid turnover and induction of phenylalanine ammonia-lyase (PAL) activity in tobacco suspension culture cells were investigated. Tobacco cells labeled by [3H]inositol in vivo were treated with Phytophthora nicotianae elicitor and [3H]metabolites of inositol phospholipid turnover were examined. Stimulation of inositol phospholipid turnover was observed preceding the induction of PAL activity; inositol 1,4-bisphosphate increased 15 times over the control 10 min after the elicitor treatment. Increase of inositol 1,4,5-trisphosphate was only 38% of the control. Phosphatidylinositol and phosphatidylinositol 4-phosphate transiently decreased by 21 and 35%, respectively. Phosphatidylinositol 4,5-bisphosphate was not affected significantly by the elicitor. Inositol 1,4-bisphosphate was preferentially elevated by elicitation than 1,4,5-trisphosphate suggesting that the regulatory mechanism of inositol phospholipid turnover in tobacco cells is different from that in animal cells. Phosphatidylinositol kinase but not phospholipase C was activated by the elicitor in vitro. Elicitor-dose dependency curves in the induction of PAL activity and in the stimulation of inositol phospholipid turnover showed a similar feature suggesting that inositol phospholipid turnover is involved in the elicitor-signal transduction in tobacco cells.
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Claims 13
Claim · model-written summary
Fungal elicitor stimulated inositol phospholipid turnover in tobacco suspension cells before PAL activity was induced.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Treatment
- Phytophthora nicotianae elicitore6
- Interactor
- Phytophthora nicotianae
Phytophthora nicotianaee6pathogen - Outcome
- inositol phospholipid turnovere0
- Direction
- Stimulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Stimulation of inositol phospholipid turnover was observed preceding the induction of PAL activity”
e6“treated with Phytophthora nicotianae elicitor”
Claim · model-written summary
Fungal elicitor induced phenylalanine ammonia-lyase activity in tobacco suspension cells.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Phytophthora nicotianae elicitore6
- Interactor
- Phytophthora nicotianaePhytophthora nicotianaee6pathogen
- Outcome
- PAL activitye0
- Direction
- inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Stimulation of inositol phospholipid turnover was observed preceding the induction of PAL activity”
e6“treated with Phytophthora nicotianae elicitor”
Claim · model-written summary
Inositol 1,4-bisphosphate increased 15 times over the control 10 min after elicitor treatment.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- elicitor treatmente0
- Interactor
- Phytophthora nicotianaePhytophthora nicotianaee6pathogen
- Outcome
- inositol 1,4-bisphosphatee0
- Direction
- increasede0
- Value
- 15 timese0
- Qualifier
- 10 min aftere0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“inositol 1,4-bisphosphate increased 15 times over the control 10 min after the elicitor treatment”
e6“treated with Phytophthora nicotianae elicitor”
Claim · model-written summary
The increase of inositol 1,4,5-trisphosphate after elicitor treatment was only 38% of the control.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Phytophthora nicotianae elicitore6
- Interactor
- Phytophthora nicotianaePhytophthora nicotianaee6pathogen
- Outcome
- inositol 1,4,5-trisphosphatee0
- Direction
- Increasee0
- Value
- 38% of the controle0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Increase of inositol 1,4,5-trisphosphate was only 38% of the control”
e6“treated with Phytophthora nicotianae elicitor”
Claim · model-written summary
Phosphatidylinositol transiently decreased by 21% after elicitor treatment.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Phytophthora nicotianae elicitore6
- Interactor
- Phytophthora nicotianaePhytophthora nicotianaee6pathogen
- Outcome
- Phosphatidylinositole0
- Direction
- decreasede0
- Value
- 21e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Phosphatidylinositol and phosphatidylinositol 4-phosphate transiently decreased by 21 and 35%, respectively”
e6“treated with Phytophthora nicotianae elicitor”
Claim · model-written summary
Phosphatidylinositol 4-phosphate transiently decreased by 35% after elicitor treatment.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Phytophthora nicotianae elicitore6
- Interactor
- Phytophthora nicotianaePhytophthora nicotianaee6pathogen
- Outcome
- phosphatidylinositol 4-phosphatee0
- Direction
- decreasede0
- Value
- 35%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Phosphatidylinositol and phosphatidylinositol 4-phosphate transiently decreased by 21 and 35%, respectively”
e6“treated with Phytophthora nicotianae elicitor”
Claim · model-written summary
Phosphatidylinositol 4,5-bisphosphate was not significantly affected by the elicitor.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- the elicitore0
- Interactor
- Phytophthora nicotianaePhytophthora nicotianaee6pathogen
- Outcome
- Phosphatidylinositol 4,5-bisphosphatee0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Phosphatidylinositol 4,5-bisphosphate was not affected significantly by the elicitor”
e6“treated with Phytophthora nicotianae elicitor”
Claim · model-written summary
Phosphatidylinositol kinase was activated by the elicitor in vitro.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- the elicitore0
- Gene
- Phosphatidylinositol kinasee0
- Outcome
- Phosphatidylinositol kinasee0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Phosphatidylinositol kinase but not phospholipase C was activated by the elicitor in vitro”
Claim · model-written summary
Phospholipase C was not activated by the elicitor in vitro.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- the elicitore0
- Gene
- phospholipase Ce0
- Outcome
- phospholipase Ce0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Phosphatidylinositol kinase but not phospholipase C was activated by the elicitor in vitro”
Claim · model-written summary
Inositol 1,4-bisphosphate was elevated more than inositol 1,4,5-trisphosphate by elicitation.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- elicitatione0
- Interactor
- Phytophthora nicotianaePhytophthora nicotianaee6pathogen
- Outcome
- Inositol 1,4-bisphosphatee0
- Direction
- elevatede0
- Comparator
- 1,4,5-trisphosphatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Inositol 1,4-bisphosphate was preferentially elevated by elicitation than 1,4,5-trisphosphate”
e6“treated with Phytophthora nicotianae elicitor”
Claim · model-written summary
The authors suggest the regulatory mechanism of inositol phospholipid turnover in tobacco cells differs from that in animal cells.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- regulatory mechanism of inositol phospholipid turnovere0
- Qualifier
- different from that in animal cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that the regulatory mechanism of inositol phospholipid turnover in tobacco cells is different from that in animal cells”
Claim · model-written summary
Elicitor-dose dependency curves for PAL activity induction and inositol phospholipid turnover stimulation showed a similar feature.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- Elicitor-dosee0
- Outcome
- induction of PAL activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco”
e0“Elicitor-dose dependency curves in the induction of PAL activity and in the stimulation of inositol phospholipid turnover showed a similar feature”
Claim · model-written summary
The authors suggest that inositol phospholipid turnover is involved in elicitor-signal transduction in tobacco cells.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- elicitor-signal transductione0
- Qualifier
- inositol phospholipid turnover is involvede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that inositol phospholipid turnover is involved in the elicitor-signal transduction in tobacco cells”
The same questions in other papers
resistance inducer · Phytophthora nicotianae · Nicotiana tabacum (tobacco)Positive and negative results
8 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.