Abstract
Phytophthora parasitica var. nicotianae produces a 34-kDa glycoprotein elicitor (CBEL) that is localized in the cell wall. A cDNA encoding the protein moiety of this elicitor was cloned and characterized. The deduced amino acid sequence consisted of two direct repeats of a cysteine-rich domain, joined by a Thr/Pro-rich region. Although having no general homology with published sequences, the positions of the cysteine residues in the two repeats show a conserved pattern, similar to that of the cellulose-binding domain of fungal glycanases. CBEL did not possess hydrolytic activity on a variety of glycans, but bound to fibrous cellulose and plant cell walls. In addition, it exerted a lectin-like hemagglutinating activity. Infiltration of tobacco leaves (cultivar 46-8) with this molecule elicited necrosis and defense gene expression at 150 nM. Elicitor pretreatment of this tobacco cultivar resulted in protection against subsequent inoculation with an otherwise virulent race of P. parasitica var. nicotianae. All these biological activities were exerted within a low concentration range. This is the first report that a fungal elicitor exhibits cellulose-binding and lectin-like activities. The possible implications of such a multifunctional elicitor in plant-microbe interactions are discussed.
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Claims 3
Claim · model-written summary
CBEL infiltration elicited tobacco leaf necrosis at 150 nM.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- cultivar 46-8e0
- Organ / stage
- leavese0
- Treatment
- Infiltration of tobacco leaves (cultivar 46-8) with this moleculee0
- Outcome
- necrosise0
- Value
- 150 nMe0
Not reportedInteractorComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infiltration of tobacco leaves (cultivar 46-8) with this molecule elicited necrosis and defense gene expression at 150 nM.”
Claim · model-written summary
CBEL infiltration elicited tobacco defense gene expression at 150 nM.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- cultivar 46-8e0
- Organ / stage
- leavese0
- Treatment
- Infiltration of tobacco leaves (cultivar 46-8) with this moleculee0
- Outcome
- defense gene expressione0
- Direction
- elicitede0
- Value
- 150 nMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infiltration of tobacco leaves (cultivar 46-8) with this molecule elicited necrosis and defense gene expression at 150 nM.”
Claim · model-written summary
Elicitor pretreatment protected tobacco against a virulent Phytophthora race.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Elicitor pretreatmente0
- Interactor
- P. parasitica var. nicotianae
Phytophthora nicotianaee0pathogen - Outcome
- protectione0
- Direction
- protectione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elicitor pretreatment of this tobacco cultivar resulted in protection against subsequent inoculation with an otherwise virulent race of P. parasitica var. nicotianae.”
The same questions in other papers
resistance inducer · Phytophthora nicotianae · Nicotiana tabacum (tobacco)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.