Abstract
Kinetin increased the formation of scopoletin and scopolin in tobacco tissue cultures. The formation of scopolin was increased more in the presence of both kinetin (KIN) and L-phenylalanine (Phe) in the medium than in the case of either KIN or Phe alone. The activity of L-phenylalanine ammonia-lyase (PAL) was increased by the addition of KIN. The increase of PAL activity was inhibited by actinomycin-D and cycloheximide added at the same time as the KIN, but actinomycin-D did not inhibit the increase when added at 8 hr after the addition of KIN at which time cycloheximide inhibited it still. The incorporation of [U-14C]-Phe into scopoletin and scopolin was stimulated by KIN, but its incorporation into protein and lignin was not affected. These results suggest that KIN increased de novo synthesis of PAL through its mRNA, and the enhanced activity of PAL resulted in the increase of scopoletin and scopolin formation.
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Claims 10
Claim · model-written summary
Kinetin increased scopoletin and scopolin formation in tobacco tissue cultures.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Kinetine0
- Outcome
- formation of scopoletin and scopoline0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin increased the formation of scopoletin and scopolin in tobacco tissue cultures”
Claim · model-written summary
Kinetin with L-phenylalanine increased scopolin formation more than either alone.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- both kinetin (KIN) and L-phenylalanine (Phe)e0
- Outcome
- formation of scopoline0
- Direction
- increased moree0
- Comparator
- either KIN or Phe alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The formation of scopolin was increased more in the presence of both kinetin (KIN) and L-phenylalanine (Phe) in the medium than in the case of either KIN or Phe alone”
t0“tobacco tissue cultures”
Claim · model-written summary
KIN increased PAL activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- KINe0
- Gene
- L-phenylalanine ammonia-lyase (PAL)e0
- Outcome
- activity of L-phenylalanine ammonia-lyase (PAL)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of L-phenylalanine ammonia-lyase (PAL) was increased by the addition of KIN”
t0“tobacco tissue cultures”
Claim · model-written summary
Concurrent actinomycin-D and cycloheximide inhibited the KIN-associated PAL activity increase.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- actinomycin-D and cycloheximidee0
- Gene
- PALe0
- Outcome
- increase of PAL activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase of PAL activity was inhibited by actinomycin-D and cycloheximide added at the same time as the KIN”
t0“tobacco tissue cultures”
Claim · model-written summary
Actinomycin-D added 8 hr after KIN did not inhibit PAL activity increase.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- actinomycin-De0
- Outcome
- increasee0
- Direction
- did not inhibite0
- Qualifier
- 8 hr after the addition of KINe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“actinomycin-D did not inhibit the increase when added at 8 hr after the addition of KIN”
t0“tobacco tissue cultures”
Claim · model-written summary
Cycloheximide still inhibited the increase when added 8 hr after KIN.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- cycloheximidee0
- Outcome
- inhibited it stille0
- Direction
- inhibitede0
- Qualifier
- 8 hr after the addition of KINe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at 8 hr after the addition of KIN at which time cycloheximide inhibited it still”
t0“tobacco tissue cultures”
Claim · model-written summary
KIN stimulated [U-14C]-Phe incorporation into scopoletin and scopolin.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- KINe0
- Outcome
- incorporation of [U-14C]-Phe into scopoletin and scopoline0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The incorporation of [U-14C]-Phe into scopoletin and scopolin was stimulated by KIN”
t0“tobacco tissue cultures”
Claim · model-written summary
KIN did not affect [U-14C]-Phe incorporation into protein and lignin.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- KINe1
- Outcome
- incorporation into protein and lignine0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its incorporation into protein and lignin was not affected”
e1“was stimulated by KIN”
t0“tobacco tissue cultures”
Claim · model-written summary
Authors suggest KIN increases de novo PAL synthesis through mRNA.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- PALe0
- Outcome
- de novo synthesis of PALe0
- Qualifier
- through its mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“KIN increased de novo synthesis of PAL through its mRNA”
t0“tobacco tissue cultures”
Claim · model-written summary
Authors attribute greater scopoletin and scopolin formation to enhanced PAL activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- PALe0
- Outcome
- increase of scopoletin and scopolin formatione0
- Qualifier
- enhanced activity of PALe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the enhanced activity of PAL resulted in the increase of scopoletin and scopolin formation”
t0“tobacco tissue cultures”
The same questions in other papers
kinetin · quality: protein · Nicotiana tabacum (tobacco)Results differ
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
cycloheximide · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
10 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
8 papers more in this group
See all 12 papers →actinomycin D · molecular: metabolites · Nicotiana tabacum (tobacco)Results agree
3 papers in the same direction as this paper
- negative
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.