Abstract
Rabproteins are the largest members of the small G protein family and are widely distributed in eukaryotes. It comprises eight subfamilies and is responsible for regulating vesicle transport, plant growth and development, and biotic and abiotic stress responses. In this study, the small G protein gene StRab5b was cloned from potato, and its biological information, expression profile and induced expression level, overexpression and gene silencing were examined on regulating potato resistance to Phytophthora infestans using PCR, qPCR and Virus-induced gene silencing (VIGS). Our results indicate that the amino acid of StRab5b shows the highest and lowest homology with NbRab5b in N. benthamiana and StRab in potato respectively. StRab5b expression varied among different potato tissues and varieties, and was induced by P. infestans infection. Transiently ectopic expression of StRab5b in N. benthamiana enhanced its resistance to P. infestans , whereas, silencing of StRab5b and its homologous gene facilitated pathogen infection in potato and N. benthamiana respectively. Furthermore, stable expression of the StRab5b gene in potatoes enhanced its redox-stress response capacity, as manifested by the accumulation of H 2 O 2 in infected leaves and subsequent increase in the activity and expression of ROS scavenging enzymes, thereby attenuating the development of P. infestans and ultimately reducing the lesions on infected potato leaves. In addition, the LOX gene transcripts and JA level were upregulated rapidly after inoculation with P. infestans . Collectively, our results suggest that StRab5b positively regulates the resistance against potato late blight (PLB) via JA-mediated defense signaling pathway.
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Claims 14
Claim · model-written summary
StRab5b amino acid had highest homology with NbRab5b in N. benthamiana.
- Taxon
- potato
Solanum tuberosumt0 - Gene
- StRab5be0
- Outcome
- homology with NbRab5b in N. benthamianae0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the amino acid of StRab5b shows the highest and lowest homology with NbRab5b in N. benthamiana and StRab in potato respectively”
t0“potato”
Claim · model-written summary
StRab5b amino acid had lowest homology with StRab in potato.
- Taxon
- potatoSolanum tuberosume0
- Gene
- StRab5be0
- Outcome
- lowest homology with NbRab5b in N. benthamiana and StRab in potato respectivelye0
- Direction
- loweste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the amino acid of StRab5b shows the highest and lowest homology with NbRab5b in N. benthamiana and StRab in potato respectively”
Claim · model-written summary
StRab5b expression varied among potato tissues and varieties.
- Taxon
- potatoSolanum tuberosume0
- Gene
- StRab5be0
- Outcome
- expressione0
- Qualifier
- different potato tissues and varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StRab5b expression varied among different potato tissues and varieties”
Claim · model-written summary
P. infestans infection induced StRab5b expression.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- P. infestans infectione0
- Interactor
- P. infestans
Phytophthora infestanse0pathogen - Gene
- StRab5be0
- Outcome
- StRab5b expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StRab5b expression varied among different potato tissues and varieties, and was induced by P. infestans infection”
t0“potato”
Claim · model-written summary
Ectopic StRab5b expression enhanced resistance to P. infestans in N. benthamiana.
- Taxon
- N. benthamianae0
- Treatment
- Transiently ectopic expression of StRab5be0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- StRab5be0
- Outcome
- resistance to P. infestanse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transiently ectopic expression of StRab5b in N. benthamiana enhanced its resistance to P. infestans”
Claim · model-written summary
Silencing StRab5b facilitated pathogen infection in potato.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- silencing of StRab5be0
- Interactor
- pathogene0pathogen
- Gene
- StRab5be0
- Outcome
- pathogen infectione0
- Direction
- facilitatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of StRab5b and its homologous gene facilitated pathogen infection in potato and N. benthamiana respectively”
Claim · model-written summary
Silencing the homologous gene facilitated pathogen infection in N. benthamiana.
- Taxon
- N. benthamianae0
- Treatment
- silencing of StRab5b and its homologous genee0
- Interactor
- pathogene0pathogen
- Gene
- homologous genee0
- Outcome
- pathogen infectione0
- Direction
- facilitatede0
Not reportedComparator
Verbatim
e0“silencing of StRab5b and its homologous gene facilitated pathogen infection in potato and N. benthamiana respectively”
Claim · model-written summary
Stable StRab5b expression increased H 2 O 2 accumulation in infected potato leaves.
- Taxon
- potatoesSolanum tuberosume0
- Organ / stage
- leavese0
- Treatment
- stable expression of the StRab5b genee0
- Gene
- StRab5be0
- Outcome
- accumulation of H 2 O 2e0
- Direction
- enhancede0
- Qualifier
- infected leavese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stable expression of the StRab5b gene in potatoes enhanced its redox-stress response capacity, as manifested by the accumulation of H 2 O 2 in infected leaves”
Claim · model-written summary
Stable StRab5b expression increased ROS scavenging enzyme activity and expression in potato.
- Taxon
- potatoesSolanum tuberosume0
- Treatment
- stable expression of the StRab5b genee0
- Gene
- ROS scavenging enzymese0
- Outcome
- activity and expression of ROS scavenging enzymese0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stable expression of the StRab5b gene in potatoes enhanced its redox-stress response capacity, as manifested by the accumulation of H 2 O 2 in infected leaves and subsequent increase in the activity and expression of ROS scavenging enzymes”
Claim · model-written summary
Stable StRab5b expression attenuated P. infestans development in potato.
- Taxon
- potatoesSolanum tuberosume0
- Treatment
- stable expression of the StRab5b genee0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- StRab5be0
- Outcome
- development of P. infestanse0
- Direction
- attenuatinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stable expression of the StRab5b gene in potatoes enhanced its redox-stress response capacity, as manifested by the accumulation of H 2 O 2 in infected leaves and subsequent increase in the activity and expression of ROS scavenging enzymes, thereby attenuating the development of P. infestans”
Claim · model-written summary
Stable StRab5b expression reduced lesions on infected potato leaves.
- Taxon
- potatoesSolanum tuberosume0
- Organ / stage
- leavese0
- Treatment
- stable expression of the StRab5b genee0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- StRab5be0
- Outcome
- lesions on infected potato leavese0
- Direction
- reducinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stable expression of the StRab5b gene in potatoes enhanced its redox-stress response capacity, as manifested by the accumulation of H 2 O 2 in infected leaves and subsequent increase in the activity and expression of ROS scavenging enzymes, thereby attenuating the development of P. infestans and ultimately reducing the lesions on infected potato leaves”
Claim · model-written summary
LOX transcripts rose rapidly after P. infestans inoculation.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- inoculation with P. infestanse0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- LOXe0
- Outcome
- LOX gene transcriptse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the LOX gene transcripts and JA level were upregulated rapidly after inoculation with P. infestans”
t0“potato”
Claim · model-written summary
JA level rose rapidly after P. infestans inoculation.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- inoculation with P. infestanse0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- JA levele0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the LOX gene transcripts and JA level were upregulated rapidly after inoculation with P. infestans”
t0“potato”
Claim · model-written summary
The authors infer that StRab5b regulates potato late blight resistance through JA-mediated defense signaling.
- Taxon
- potatoSolanum tuberosume0
- Gene
- StRab5be0
- Outcome
- resistance against potato late blight (PLB)e0
- Direction
- positively regulatese0
- Qualifier
- JA-mediated defense signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StRab5b positively regulates the resistance against potato late blight (PLB) via JA-mediated defense signaling pathway”
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