Abstract
The Escherichia coli glycogen branching enzyme (GLGB) was fused to either the C- or N-terminus of a starch-binding domain (SBD) and expressed in two potato genetic backgrounds: the amylose-free mutant (amf) and an amylose-containing line (Kardal). Regardless of background or construct used, a large amount of GLGB/SBD fusion protein was accumulated inside the starch granules, however, without an increase in branching. The presence of GLGB/SBD fusion proteins resulted in altered morphology of the starch granules in both genetic backgrounds. In the amf genetic background, the starch granules showed both amalgamated granules and porous starch granules, whereas in Kardal background, the starch granules showed an irregular rough surface. The altered starch granules in both amf and Kardal backgrounds were visible from the initial stage of potato tuber development. High-throughput transcriptomic analysis showed that expression of GLGB/SBD fusion protein in potato tubers did not affect the expression level of most genes directly involved in the starch biosynthesis except for the up-regulation of a beta-amylase gene in Kardal background. The beta-amylase protein could be responsible for the degradation of the extra branches potentially introduced by GLGB.
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Claims 8
Claim · model-written summary
GLGB/SBD fusion protein accumulated inside potato starch granules
- Taxon
- potato
Solanum tuberosumt0 - Treatment
- GLGB/SBD fusion proteine1
- Outcome
- accumulated inside the starch granulese1
- Direction
- accumulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in potato results in severe morphological changes in starch granules”
e1“Regardless of background or construct used, a large amount of GLGB/SBD fusion protein was accumulated inside the starch granules, however, without an increase in branching”
Claim · model-written summary
GLGB/SBD fusion protein did not increase branching in potato starch
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- GLGB/SBD fusion proteine1
- Outcome
- branchinge1
- Direction
- without an increasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in potato results in severe morphological changes in starch granules”
e1“Regardless of background or construct used, a large amount of GLGB/SBD fusion protein was accumulated inside the starch granules, however, without an increase in branching”
Claim · model-written summary
GLGB/SBD fusion proteins altered starch granule morphology in the amf background
- Taxon
- potatoSolanum tuberosumt0
- Material
- amfe2
- Treatment
- GLGB/SBD fusion proteinse1
- Outcome
- morphology of the starch granulese1
- Direction
- alterede1
- Qualifier
- amalgamated granules and porous starch granulese2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in potato results in severe morphological changes in starch granules”
e1“The presence of GLGB/SBD fusion proteins resulted in altered morphology of the starch granules in both genetic backgrounds”
e2“In the amf genetic background, the starch granules showed both amalgamated granules and porous starch granules”
Claim · model-written summary
GLGB/SBD fusion proteins altered starch granule morphology in the Kardal background
- Taxon
- potatoSolanum tuberosumt0
- Material
- Kardale3
- Treatment
- GLGB/SBD fusion proteinse1
- Outcome
- morphology of the starch granulese1
- Direction
- alterede1
- Qualifier
- irregular rough surfacee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in potato results in severe morphological changes in starch granules”
e1“The presence of GLGB/SBD fusion proteins resulted in altered morphology of the starch granules in both genetic backgrounds”
e3“in Kardal background, the starch granules showed an irregular rough surface”
Claim · model-written summary
Altered starch granules were visible from the initial stage of potato tuber development in amf and Kardal
- Taxon
- potatoSolanum tuberosumt0
- Material
- amf and Kardale1
- Organ / stage
- initial stage of potato tuber developmente1
- Outcome
- altered starch granulese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in potato results in severe morphological changes in starch granules”
e1“The altered starch granules in both amf and Kardal backgrounds were visible from the initial stage of potato tuber development”
Claim · model-written summary
GLGB/SBD expression did not affect the expression of most starch biosynthesis genes in tubers
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- potato tuberse1
- Treatment
- expression of GLGB/SBD fusion protein in potato tuberse1
- Outcome
- expression level of most genes directly involved in the starch biosynthesise1
- Direction
- did not affecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in potato results in severe morphological changes in starch granules”
e1“High-throughput transcriptomic analysis showed that expression of GLGB/SBD fusion protein in potato tubers did not affect the expression level of most genes directly involved in the starch biosynthesis except for the up-regulation of a beta-amylase gene in Kardal background”
Claim · model-written summary
GLGB/SBD expression up-regulated a beta-amylase gene in the Kardal background
- Taxon
- potatoSolanum tuberosumt0
- Material
- Kardale1
- Treatment
- expression of GLGB/SBD fusion protein in potato tuberse1
- Gene
- beta-amylasee1
- Outcome
- beta-amylase genee1
- Direction
- up-regulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in potato results in severe morphological changes in starch granules”
e1“High-throughput transcriptomic analysis showed that expression of GLGB/SBD fusion protein in potato tubers did not affect the expression level of most genes directly involved in the starch biosynthesis except for the up-regulation of a beta-amylase gene in Kardal background”
Claim · model-written summary
The beta-amylase protein may degrade the extra branches introduced by GLGB
- Taxon
- potatoSolanum tuberosumt0
- Gene
- beta-amylase proteine1
- Outcome
- degradation of the extra branchese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in potato results in severe morphological changes in starch granules”
e1“The beta-amylase protein could be responsible for the degradation of the extra branches potentially introduced by GLGB”
The same questions in other papers
transgenic · molecular: expression · Solanum tuberosum (potato)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.