Abstract
Late blight caused by Phytophthora infestans is a major threat to global potato and tomato production. Sustainable management of late blight requires the development of resistant crop cultivars. This process can be facilitated by high-throughput identification of functional genes involved in late blight pathogenesis. In this study, we generated a high-quality transcriptomic time-course dataset focusing on the initial 24 h of contact between P. infestans and 3 solanaceous plant species, tobacco (Nicotiana benthamiana), tomato (Solanum lycopersicum), and potato (Solanum tuberosum). Our results demonstrate species-specific transcriptional regulation in early stages of the infection. Transient silencing of putative RIBOSE-5-PHOSPHATE ISOMERASE and HMG-CoA REDUCTASE genes in N. benthamiana lowered plant resistance against P. infestans. Furthermore, heterologous expression of a putative tomato Golgi-localized nucleosugar transporter-encoding gene exacerbated P. infestans infection of N. benthamiana. In comparison, bioassays using transgenic tomato lines showed that the quantitative disease resistance genes were required but insufficient for late blight resistance; genetic knock-out of the susceptibility gene enhanced resistance. The same RNA-seq dataset was exploited to examine the transcriptional landscape of P. infestans and revealed host-specific gene expression patterns in the pathogen. This temporal transcriptomic diversity, in combination with genomic distribution features, identified the P. infestans IPI-B family GLYCINE-RICH PROTEINs as putative virulence factors that promoted disease severity or induced plant tissue necrosis when transiently expressed in N. benthamiana. These functional genes underline the effectiveness of functional gene-mining through a time-course dual RNA-seq approach and provide insight into the molecular interactions between solanaceous plants and P. infestans.
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Claims 10
Claim · model-written summary
A high-quality early contact transcriptomic time-course dataset was generated.
- Taxon
- tobacco (Nicotiana benthamiana), tomato (Solanum lycopersicum), and potato (Solanum tuberosum)
Solanum lycopersicum
Nicotiana benthamiana
Solanum tuberosume0 - Interactor
- P. infestans
Phytophthora infestanse0pathogen - Outcome
- transcriptomic time-course datasete0
- Value
- 24 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we generated a high-quality transcriptomic time-course dataset focusing on the initial 24 h of contact between P. infestans and 3 solanaceous plant species, tobacco (Nicotiana benthamiana), tomato (Solanum lycopersicum), and potato (Solanum tuberosum).”
Claim · model-written summary
Early infection transcriptional regulation differed by plant species.
- Taxon
- tobacco (Nicotiana benthamiana), tomato (Solanum lycopersicum), and potato (Solanum tuberosum)Solanum lycopersicumNicotiana benthamianaSolanum tuberosume1
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogene0
- Outcome
- species-specific transcriptional regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results demonstrate species-specific transcriptional regulation in early stages of the infection.”
t0“plant-Phytophthora infestans interactions”
e1“we generated a high-quality transcriptomic time-course dataset focusing on the initial 24 h of contact between P. infestans and 3 solanaceous plant species, tobacco (Nicotiana benthamiana), tomato (Solanum lycopersicum), and potato (Solanum tuberosum).”
Claim · model-written summary
Silencing putative RIBOSE-5-PHOSPHATE ISOMERASE lowered tobacco resistance to P. infestans.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Transient silencinge0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- RIBOSE-5-PHOSPHATE ISOMERASEe0
- Outcome
- plant resistance against P. infestanse0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient silencing of putative RIBOSE-5-PHOSPHATE ISOMERASE and HMG-CoA REDUCTASE genes in N. benthamiana lowered plant resistance against P. infestans.”
Claim · model-written summary
Silencing putative HMG-CoA REDUCTASE lowered tobacco resistance to P. infestans.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Transient silencinge0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- HMG-CoA REDUCTASEe0
- Outcome
- plant resistance against P. infestanse0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient silencing of putative RIBOSE-5-PHOSPHATE ISOMERASE and HMG-CoA REDUCTASE genes in N. benthamiana lowered plant resistance against P. infestans.”
Claim · model-written summary
Expression of a tomato nucleosugar transporter gene worsened P. infestans infection of tobacco.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- heterologous expressione0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- tomato Golgi-localized nucleosugar transporter-encoding genee0
- Outcome
- P. infestans infectione0
- Direction
- exacerbatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heterologous expression of a putative tomato Golgi-localized nucleosugar transporter-encoding gene exacerbated P. infestans infection of N. benthamiana.”
Claim · model-written summary
Quantitative disease resistance genes were required but insufficient for late blight resistance in tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Material
- transgenic tomato linese0
- Gene
- quantitative disease resistance genese0
- Outcome
- late blight resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bioassays using transgenic tomato lines showed that the quantitative disease resistance genes were required but insufficient for late blight resistance”
Claim · model-written summary
Knocking out the susceptibility gene enhanced tomato resistance.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- genetic knock-oute0
- Interactor
- Phytophthora infestansPhytophthora infestanst0pathogene0
- Gene
- susceptibility genee0
- Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetic knock-out of the susceptibility gene enhanced resistance.”
t0“plant-Phytophthora infestans interactions”
e1“we generated a high-quality transcriptomic time-course dataset focusing on the initial 24 h of contact between P. infestans and 3 solanaceous plant species, tobacco (Nicotiana benthamiana), tomato (Solanum lycopersicum), and potato (Solanum tuberosum).”
Claim · model-written summary
Pathogen transcription showed host-specific expression patterns.
- Taxon
- tobacco (Nicotiana benthamiana), tomato (Solanum lycopersicum), and potato (Solanum tuberosum)Solanum lycopersicumNicotiana benthamianaSolanum tuberosume1
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Outcome
- host-specific gene expression patterns in the pathogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The same RNA-seq dataset was exploited to examine the transcriptional landscape of P. infestans and revealed host-specific gene expression patterns in the pathogen.”
t0“plant-Phytophthora infestans interactions”
e1“we generated a high-quality transcriptomic time-course dataset focusing on the initial 24 h of contact between P. infestans and 3 solanaceous plant species, tobacco (Nicotiana benthamiana), tomato (Solanum lycopersicum), and potato (Solanum tuberosum).”
Claim · model-written summary
IPI-B family glycine-rich proteins were identified as putative virulence factors.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- P. infestans IPI-B family GLYCINE-RICH PROTEINse0
- Outcome
- putative virulence factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified the P. infestans IPI-B family GLYCINE-RICH PROTEINs as putative virulence factors”
t0“plant-Phytophthora infestans interactions”
e1“Transient silencing of putative RIBOSE-5-PHOSPHATE ISOMERASE and HMG-CoA REDUCTASE genes in N. benthamiana lowered plant resistance against P. infestans.”
Claim · model-written summary
Transient expression of pathogen glycine-rich proteins promoted disease severity or tissue necrosis in tobacco.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- transiently expressede0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- P. infestans IPI-B family GLYCINE-RICH PROTEINse0
- Outcome
- disease severity or induced plant tissue necrosise0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. infestans IPI-B family GLYCINE-RICH PROTEINs as putative virulence factors that promoted disease severity or induced plant tissue necrosis when transiently expressed in N. benthamiana.”
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