Abstract
BACKGROUND: Small GTPases are monomeric guanine nucleotide-binding proteins. In plants, ROPs regulate plant cell polarity, plant cell differentiation and development as well as biotic and abiotic stress signaling pathways. RESULTS: We report the subcellular localization of the AtRop1 protein at the plasma membrane in tobacco epidermal cells using GFP fusions. Additionally, transient and stable expression of a dominant negative form (DN) of the Arabidopsis AtRop1 in potato led to H2O2 accumulation associated with the reduced development of Phytophthora infestans Montagne de Bary and smaller lesions on infected potato leaves. The expression of the Strboh-D gene, a NADPH oxidase homologue in potato, was analyzed by RT-PCR. Expression of this gene was maintained in DN-AtRop1 transgenic plants after infection with P. infestans. In transgenic potato lines, the transcript levels of salicylic acid (SA) and jasmonic acid (JA) marker genes (Npr1 and Lox, respectively) were analyzed. The Lox gene was induced dramatically whereas expression of Npr1, a gene up-regulated by SA, decreased slightly in DN-AtRop1 transgenic plants after infection with P. infestans. CONCLUSIONS: In conclusion, our results indicate that DN-AtROP1 affects potato resistance to P. infestans. This is associated with increased NADPH oxidase-mediated H2O2 production and JA signaling.
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Claims 8
Claim · model-written summary
AtRop1 localized to the plasma membrane in tobacco epidermal cells.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- epidermal cellse0
- Gene
- AtRop1e0
- Outcome
- plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the subcellular localization of the AtRop1 protein at the plasma membrane in tobacco epidermal cells using GFP fusions.”
Claim · model-written summary
Dominant-negative AtRop1 expression in potato was associated with H2O2 accumulation.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- transient and stable expression of a dominant negative form (DN) of the Arabidopsis AtRop1e0
- Interactor
- Phytophthora infestans Montagne de Bary
Phytophthora infestanse0pathogen - Gene
- Arabidopsis AtRop1e0
- Outcome
- H2O2 accumulatione0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient and stable expression of a dominant negative form (DN) of the Arabidopsis AtRop1 in potato led to H2O2 accumulation associated with the reduced development of Phytophthora infestans Montagne de Bary and smaller lesions on infected potato leaves.”
Claim · model-written summary
Dominant-negative AtRop1 expression reduced development of Phytophthora infestans in potato.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- transient and stable expression of a dominant negative form (DN) of the Arabidopsis AtRop1e0
- Interactor
- Phytophthora infestans Montagne de BaryPhytophthora infestanse0pathogen
- Gene
- Arabidopsis AtRop1e0
- Outcome
- development of Phytophthora infestans Montagne de Barye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient and stable expression of a dominant negative form (DN) of the Arabidopsis AtRop1 in potato led to H2O2 accumulation associated with the reduced development of Phytophthora infestans Montagne de Bary and smaller lesions on infected potato leaves.”
Claim · model-written summary
Dominant-negative AtRop1 expression produced smaller lesions on infected potato leaves.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- transient and stable expression of a dominant negative form (DN) of the Arabidopsis AtRop1e0
- Interactor
- Phytophthora infestans Montagne de BaryPhytophthora infestanse0pathogen
- Gene
- Arabidopsis AtRop1e0
- Outcome
- lesions on infected potato leavese0
- Direction
- smallere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient and stable expression of a dominant negative form (DN) of the Arabidopsis AtRop1 in potato led to H2O2 accumulation associated with the reduced development of Phytophthora infestans Montagne de Bary and smaller lesions on infected potato leaves.”
Claim · model-written summary
Strboh-D expression was maintained in dominant-negative AtRop1 potato after infection.
- Taxon
- potatoSolanum tuberosumt0
- Material
- DN-AtRop1 transgenic plantse0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- Strboh-De1
- Outcome
- Expression of this genee0
- Direction
- maintainede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of this gene was maintained in DN-AtRop1 transgenic plants after infection with P. infestans.”
e1“The expression of the Strboh-D gene, a NADPH oxidase homologue in potato, was analyzed by RT-PCR.”
t0“potato”
Claim · model-written summary
Lox expression was strongly induced in dominant-negative AtRop1 potato after infection.
- Taxon
- potatoSolanum tuberosumt0
- Material
- DN-AtRop1 transgenic plantse0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- Loxe0
- Outcome
- Lox genee0
- Direction
- induced dramaticallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Lox gene was induced dramatically whereas expression of Npr1, a gene up-regulated by SA, decreased slightly in DN-AtRop1 transgenic plants after infection with P. infestans.”
t0“potato”
Claim · model-written summary
Npr1 expression decreased slightly in dominant-negative AtRop1 potato after infection.
- Taxon
- potatoSolanum tuberosumt0
- Material
- DN-AtRop1 transgenic plantse0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- Npr1e0
- Outcome
- expression of Npr1e0
- Direction
- decreased slightlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Lox gene was induced dramatically whereas expression of Npr1, a gene up-regulated by SA, decreased slightly in DN-AtRop1 transgenic plants after infection with P. infestans.”
t0“potato”
Claim · model-written summary
The authors associate altered potato resistance with increased NADPH oxidase-mediated H2O2 production and JA signaling.
- Taxon
- potatoSolanum tuberosumt0
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Outcome
- potato resistancet0
- Qualifier
- increased NADPH oxidase-mediated H2O2 production and JA signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This is associated with increased NADPH oxidase-mediated H2O2 production and JA signaling.”
t0“potato resistance to Phytophthora infestans”
e1“DN-AtROP1 affects potato resistance to P. infestans.”
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transgenic · Phytophthora infestans · Solanum tuberosum (potato)Positive and negative results
9 papers in the same direction as this paper · 5 in the opposite direction
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.