Abstract
The aim of this work was to establish whether plastidial phosphoglucomutase is involved in the starch biosynthetic pathway of potato tubers and thereby to determine the form in which carbon is imported into the potato amyloplast. For this purpose, we cloned the plastidial isoform of potato PGM (StpPGM), and using an antisense approach generated transgenic potato plants that exhibited decreased expression of the StpPGM gene and contained significantly reduced total phosphoglucomutase activity. We confirmed that this loss in activity was due specifically to a reduction in plastidial PGM activity. Potato lines with decreased activities of plastidial PGM exhibited no major changes in either whole-plant or tuber morphology. However, tubers from these lines exhibited a dramatic (up to 40%) decrease in the accumulation of starch, and significant increases in the levels of sucrose and hexose phosphates. As tubers from these lines exhibited no changes in the maximal catalytic activities of other key enzymes of carbohydrate metabolism, we conclude that plastidial PGM forms part of the starch biosynthetic pathway of the potato tuber, and that glucose-6-phosphate is the major precursor taken up by amyloplasts in order to support starch synthesis.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
The potato plastidial PGM isoform StpPGM was cloned.
- Taxon
- potato
Solanum tuberosume0 - Gene
- StpPGMe0
- Outcome
- cloned the plastidial isoforme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we cloned the plastidial isoform of potato PGM (StpPGM)”
Claim · model-written summary
Antisense potato plants showed decreased StpPGM gene expression.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- transgenic potato plantse0
- Gene
- StpPGMe0
- Outcome
- expression of the StpPGM genee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“generated transgenic potato plants that exhibited decreased expression of the StpPGM gene and contained significantly reduced total phosphoglucomutase activity.”
Claim · model-written summary
Antisense potato plants had significantly reduced total phosphoglucomutase activity.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- transgenic potato plantse0
- Gene
- StpPGMe0
- Outcome
- total phosphoglucomutase activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“generated transgenic potato plants that exhibited decreased expression of the StpPGM gene and contained significantly reduced total phosphoglucomutase activity.”
Claim · model-written summary
The loss of activity was specifically a reduction in plastidial PGM activity.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- plastidial PGMe0
- Outcome
- plastidial PGM activitye0
- Direction
- reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We confirmed that this loss in activity was due specifically to a reduction in plastidial PGM activity.”
t0“potato”
Claim · model-written summary
Potato lines with reduced plastidial PGM activity had no major changes in whole plant or tuber morphology.
- Taxon
- PotatoSolanum tuberosume0
- Gene
- plastidial PGMe0
- Outcome
- whole-plant or tuber morphologye0
- Direction
- no major changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Potato lines with decreased activities of plastidial PGM exhibited no major changes in either whole-plant or tuber morphology.”
Claim · model-written summary
Tubers with reduced plastidial PGM activity accumulated up to 40% less starch.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- tuberse0
- Gene
- plastidial phosphoglucomutaset0
- Outcome
- accumulation of starche0
- Direction
- decreasee0
- Value
- up to 40%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, tubers from these lines exhibited a dramatic (up to 40%) decrease in the accumulation of starch, and significant increases in the levels of sucrose and hexose phosphates.”
t0“plastidial phosphoglucomutase provides compelling evidence that potato”
Claim · model-written summary
Tubers with reduced plastidial PGM activity had significantly increased sucrose levels.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- tuberse0
- Gene
- plastidial phosphoglucomutaset0
- Outcome
- levels of sucrosee0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, tubers from these lines exhibited a dramatic (up to 40%) decrease in the accumulation of starch, and significant increases in the levels of sucrose and hexose phosphates.”
t0“plastidial phosphoglucomutase provides compelling evidence that potato”
Claim · model-written summary
Tubers with reduced plastidial PGM activity had significantly increased hexose phosphate levels.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- tuberse0
- Gene
- plastidial phosphoglucomutaset0
- Outcome
- hexose phosphatese0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, tubers from these lines exhibited a dramatic (up to 40%) decrease in the accumulation of starch, and significant increases in the levels of sucrose and hexose phosphates.”
t0“plastidial phosphoglucomutase provides compelling evidence that potato”
Claim · model-written summary
Other key carbohydrate metabolism enzymes had unchanged maximal catalytic activities in tubers from the antisense lines.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- tuberse0
- Outcome
- maximal catalytic activities of other key enzymes of carbohydrate metabolisme0
- Direction
- no changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tubers from these lines exhibited no changes in the maximal catalytic activities of other key enzymes of carbohydrate metabolism”
t0“potato”
Claim · model-written summary
The authors conclude that plastidial PGM participates in potato tuber starch biosynthesis.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- tubere0
- Gene
- plastidial PGMe0
- Outcome
- starch biosynthetic pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we conclude that plastidial PGM forms part of the starch biosynthetic pathway of the potato tuber”
Claim · model-written summary
The authors conclude that glucose-6-phosphate is the major carbon precursor imported into amyloplasts for starch synthesis.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- amyloplastse0
- Compound
- glucose-6-phosphatee0
- Outcome
- major precursor taken up by amyloplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“glucose-6-phosphate is the major precursor taken up by amyloplasts in order to support starch synthesis.”
t0“potato”
The same questions in other papers
transgenic · physiology: enzyme activity · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- no effect
transgenic · molecular: expression · Solanum tuberosum (potato)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.