Abstract
Ralstonia solanacearum is one of the most destructive plant-pathogenic bacteria, infecting more than 200 plant species, including potato (Solanum tuberosum) and many other solanaceous crops. R. solanacearum has numerous pathogenicity factors, and type III effectors secreted through type III secretion system (T3SS) are key factors to counteract host immunity. Here, we show that RipBT is a novel T3SS-secreted effector by using a cyaA reporter system. Transient expression of RipBT in Nicotiania benthamiana induced strong cell death in a plasma membrane-localization dependent manner. Notably, mutation of RipBT in R. solanacearum showed attenuated virulence on potato, while RipBT transgenic potato plants exhibited enhanced susceptibility to R. solanacearum. Interestingly, transcriptomic analyses suggest that RipBT may interfere with plant reactive oxygen species (ROS) metabolism during the R. solanacearum infection of potato roots. In addition, the expression of RipBT remarkably suppressed the flg22-induced pathogen-associated molecular pattern-triggered immunity responses, such as the ROS burst. Taken together, RipBT acts as a T3SS effector, promoting R. solanacearum infection on potato and presumably disturbing ROS homeostasis.
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Claims 6
Claim · model-written summary
RipBT was identified as a T3SS-secreted effector.
- Taxon
- potato
Solanum tuberosumt0 - Gene
- RipBTe0
- Outcome
- novel T3SS-secreted effectore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RipBT is a novel T3SS-secreted effector by using a cyaA reporter system.”
t0“potato”
Claim · model-written summary
Transient RipBT expression caused strong cell death in Nicotiania benthamiana dependent on plasma membrane localization.
- Taxon
- Nicotiania benthamianae0
- Treatment
- Transient expression of RipBTe0
- Gene
- RipBTe0
- Outcome
- cell deathe0
- Direction
- inducede0
- Qualifier
- plasma membrane-localization dependent mannere0
Not reportedComparator
Verbatim
e0“Transient expression of RipBT in Nicotiania benthamiana induced strong cell death in a plasma membrane-localization dependent manner.”
Claim · model-written summary
RipBT mutation attenuated R. solanacearum virulence on potato.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- mutation of RipBTe0
- Interactor
- R. solanacearum
Ralstonia solanacearume0pathogen - Gene
- RipBTe0
- Outcome
- virulence on potatoe0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutation of RipBT in R. solanacearum showed attenuated virulence on potato”
Claim · model-written summary
RipBT transgenic potato showed enhanced susceptibility to R. solanacearum.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- RipBT transgenice0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Gene
- RipBTe0
- Outcome
- susceptibility to R. solanacearume0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RipBT transgenic potato plants exhibited enhanced susceptibility to R. solanacearum.”
Claim · model-written summary
Transcriptomes suggest RipBT may interfere with ROS metabolism during R. solanacearum infection of potato roots.
- Taxon
- potatoSolanum tuberosume0
- Organ / stage
- rootse0
- Interactor
- R. solanacearumRalstonia solanacearume0pathogen
- Gene
- RipBTe0
- Outcome
- plant reactive oxygen species (ROS) metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcriptomic analyses suggest that RipBT may interfere with plant reactive oxygen species (ROS) metabolism during the R. solanacearum infection of potato roots.”
Claim · model-written summary
RipBT expression suppressed flg22-induced immunity responses including ROS burst.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- expression of RipBTe0
- Gene
- RipBTe0
- Outcome
- flg22-induced pathogen-associated molecular pattern-triggered immunity responses, such as the ROS burste0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of RipBT remarkably suppressed the flg22-induced pathogen-associated molecular pattern-triggered immunity responses, such as the ROS burst.”
t0“potato”
The same questions in other papers
transgenic · physiology: oxidative · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →loss of function · Ralstonia solanacearum · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.