Abstract
Effects of the desA gene from the cyanobacterium Synechocystis sp. encoding Δ12 acyl-lipid desaturase and increasing the level of unsaturated fatty acids (linoleic acid (18:2) primarily) in membrane lipids, which was inserted into potato ( Solanum tuberosum L., cv. Desnitsa) plants, on chloroplast ultrastructure and plant tolerance to low temperatures were studied. The main attention was focused on modifications in the chloroplast structure and their possible relation to potato plant tolerance to oxidative and low-temperature stresses under the influence to their transformation with the Δ12 acyl-lipid desaturase gene from cyanobacterium ( desA-licBM3 -plants). Morphometric analysis showed that, in comparison with wild-type (WT) plants, in desA-licBM3 -plants the number of grana in chloroplasts increased substantially. The total number of thylakoids in transformant chloroplasts was almost twice higher than in WT plants. The number of plastoglobules per chloroplast of transformed plants increased by 25%. A marked increase in the number of grana, total number of thylakoids, and the number of plastoglobules in chloroplasts of desA-licBM3 -plants indicates their more intense lipid metabolism, as compared with WT plants, and this resulted in the conservation of some part of lipids in plastoglobules. In addition, the expression of heterological desA gene encoding Δ12 acyl-lipid desaturase positively influenced stabilization of not only structure but also functioning of chloroplast membranes, thus preventing a transfer of electrons from the ETR to oxygen and subsequent ROS generation at hypothermia. This was confirmed by the analysis of the rate of superoxide anion generation in tested genotypes.
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Claims 6
Claim · model-written summary
In desA-transformed potato the number of grana per chloroplast increased substantially compared with wild-type plants.
- Taxon
- potato
Solanum tuberosume2 - Material
- cv. Desnitsae2
- Treatment
- desA genee0
- Gene
- desAe0
- Outcome
- number of grana in chloroplastse1
- Direction
- increasede1
- Comparator
- wild-type (WT) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Effects of the desA gene from the cyanobacterium Synechocystis sp.”
e1“Morphometric analysis showed that, in comparison with wild-type (WT) plants, in desA-licBM3 -plants the number of grana in chloroplasts increased substantially”
e2“which was inserted into potato ( Solanum tuberosum L., cv. Desnitsa) plants”
Claim · model-written summary
The total number of thylakoids per chloroplast in desA-transformed potato was almost twice higher than in wild-type plants.
- Taxon
- potatoSolanum tuberosume3
- Treatment
- desA genee0
- Gene
- desAe0
- Outcome
- total number of thylakoids in transformant chloroplastse2
- Direction
- highere2
- Value
- almost twicee2
- Comparator
- WT plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Effects of the desA gene from the cyanobacterium Synechocystis sp.”
e2“The total number of thylakoids in transformant chloroplasts was almost twice higher than in WT plants”
e3“which was inserted into potato ( Solanum tuberosum L., cv. Desnitsa) plants”
Claim · model-written summary
The number of plastoglobules per chloroplast in desA-transformed potato increased by 25% compared with wild-type plants.
- Taxon
- potatoSolanum tuberosume4
- Treatment
- desA genee0
- Gene
- desAe0
- Outcome
- number of plastoglobules per chloroplaste3
- Direction
- increasede3
- Value
- 25%e3
- Comparator
- wild-type (WT) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Effects of the desA gene from the cyanobacterium Synechocystis sp.”
e1“Morphometric analysis showed that, in comparison with wild-type (WT) plants, in desA-licBM3 -plants the number of grana in chloroplasts increased substantially”
e3“The number of plastoglobules per chloroplast of transformed plants increased by 25%”
e4“which was inserted into potato ( Solanum tuberosum L., cv. Desnitsa) plants”
Claim · model-written summary
Increased grana, thylakoids and plastoglobules in desA-licBM3 potato indicate more intense lipid metabolism, which conserved part of the lipids in plastoglobules.
- Taxon
- potatoSolanum tuberosume6
- Outcome
- the conservation of some part of lipids in plastoglobulese5
- Qualifier
- more intense lipid metabolisme4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“A marked increase in the number of grana, total number of thylakoids, and the number of plastoglobules in chloroplasts of desA-licBM3 -plants indicates their more intense lipid metabolism”
e5“this resulted in the conservation of some part of lipids in plastoglobules”
e6“which was inserted into potato ( Solanum tuberosum L., cv. Desnitsa) plants”
Claim · model-written summary
Expression of the heterologous desA gene positively influenced the stabilisation of both structure and function of chloroplast membranes in potato.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- expression of heterological desA genee6
- Outcome
- stabilization of not only structure but also functioning of chloroplast membranese7
- Direction
- positively influencede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“the expression of heterological desA gene”
e7“positively influenced stabilization of not only structure but also functioning of chloroplast membranes”
e0“which was inserted into potato ( Solanum tuberosum L., cv. Desnitsa) plants”
Claim · model-written summary
Expression of the heterologous desA gene prevented electron transfer from the ETR to oxygen and ROS generation in potato at hypothermia.
- Taxon
- potatoSolanum tuberosume2
- Treatment
- expression of heterological desA genee6
- Outcome
- transfer of electrons from the ETR to oxygen and subsequent ROS generatione1
- Direction
- preventinge1
- Qualifier
- at hypothermiae1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“the expression of heterological desA gene”
e1“preventing a transfer of electrons from the ETR to oxygen and subsequent ROS generation at hypothermia”
e2“which was inserted into potato ( Solanum tuberosum L., cv. Desnitsa) plants”
The same questions in other papers
transgenic · physiology: oxidative · Solanum tuberosum (potato)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
2 papers more in this group
See all 6 papers →transgenic · soil: physics · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.