Abstract
Salicylic acid (SA) signalling plays an essential role in plant innate immunity. In this study, we identified a component in the SA signaling pathway in potato (Solanum tuberosum), the transcription factor StbZIP61, and characterized its function in defence against Phytophthora infestans. Expression of StbZIP61 was induced upon P. infestans infection and following exposure to the defense signaling hormones SA, ethylene and jasmonic acid. Overexpression of StbZIP61 increased the tolerance of potato plants to P. infestans while RNA interference (RNAi) increased susceptibility. Yeast two-hybrid and pull down experiments revealed that StbZIP61 could interact with an NPR3-like protein (StNPR3L) that inhibited its DNA-binding and transcriptional activation activities. Moreover, StNPR3L interacted with StbZIP61 in an SA-dependent manner. Among candidate genes involved in SA-regulated defense responses, StbZIP61 had a significant impact on expression of StICS1, which encodes a key enzyme for SA biosynthesis. StICS1 transcription was induced upon P. infestans infection and this responsive expression to the pathogen was reduced in StbZIP61 RNAi plants. Accordingly, StICS1 expression was remarkably enhanced in StbZIP61-overexpressing plants. Together, our data demonstrate that StbZIP61 functions in concert with StNPR3L to regulate the temporal activation of SA biosynthesis, which contributes to SA-mediated immunity against P. infestans infection in potato.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 16
Claim · model-written summary
StbZIP61 was identified as a component of the SA signaling pathway in potato.
- Taxon
- potato
Solanum tuberosume0 - Gene
- StbZIP61e0
- Outcome
- component in the SA signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified a component in the SA signaling pathway in potato (Solanum tuberosum), the transcription factor StbZIP61”
Claim · model-written summary
P. infestans infection induced StbZIP61 expression.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- P. infestans infectione0
- Interactor
- P. infestans
Phytophthora infestanse0pathogen - Gene
- StbZIP61e0
- Outcome
- Expression of StbZIP61e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of StbZIP61 was induced upon P. infestans infection”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
SA exposure induced StbZIP61 expression.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- SAe0
- Gene
- StbZIP61e0
- Outcome
- Expression of StbZIP61e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of StbZIP61 was induced upon P. infestans infection and following exposure to the defense signaling hormones SA, ethylene and jasmonic acid”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
Ethylene exposure induced StbZIP61 expression.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- ethylenee0
- Gene
- StbZIP61e0
- Outcome
- Expression of StbZIP61e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of StbZIP61 was induced upon P. infestans infection and following exposure to the defense signaling hormones SA, ethylene and jasmonic acid”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
Jasmonic acid exposure induced StbZIP61 expression.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- jasmonic acide0
- Gene
- StbZIP61e0
- Outcome
- Expression of StbZIP61e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of StbZIP61 was induced upon P. infestans infection and following exposure to the defense signaling hormones SA, ethylene and jasmonic acid”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
StbZIP61 overexpression increased potato tolerance to P. infestans.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- Overexpression of StbZIP61e0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- StbZIP61e0
- Outcome
- tolerance of potato plants to P. infestanse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of StbZIP61 increased the tolerance of potato plants to P. infestans”
Claim · model-written summary
StbZIP61 RNA interference increased potato susceptibility to P. infestans.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- RNA interference (RNAi)e0
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- StbZIP61e2
- Outcome
- susceptibilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while RNA interference (RNAi) increased susceptibility”
e1“potato plants to P. infestans”
e2“StbZIP61 RNAi plants”
Claim · model-written summary
StbZIP61 interacted with StNPR3L.
- Taxon
- potatoSolanum tuberosume1
- Gene
- StbZIP61e0
- Outcome
- interact with an NPR3-like protein (StNPR3L)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StbZIP61 could interact with an NPR3-like protein (StNPR3L)”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
StNPR3L inhibited StbZIP61 DNA binding.
- Taxon
- potatoSolanum tuberosume1
- Gene
- StNPR3Le0
- Outcome
- DNA-bindinge0
- Direction
- inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StNPR3L) that inhibited its DNA-binding and transcriptional activation activities”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
StNPR3L inhibited StbZIP61 transcriptional activation.
- Taxon
- potatoSolanum tuberosume1
- Gene
- StNPR3Le0
- Outcome
- transcriptional activation activitiese0
- Direction
- inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StNPR3L) that inhibited its DNA-binding and transcriptional activation activities”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
StNPR3L interacted with StbZIP61 in an SA-dependent manner.
- Taxon
- potatoSolanum tuberosume1
- Gene
- StNPR3Le0
- Outcome
- interacted with StbZIP61e0
- Qualifier
- SA-dependent mannere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StNPR3L interacted with StbZIP61 in an SA-dependent manner”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
StbZIP61 significantly affected StICS1 expression.
- Taxon
- potatoSolanum tuberosume1
- Gene
- StbZIP61e0
- Outcome
- expression of StICS1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StbZIP61 had a significant impact on expression of StICS1”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
P. infestans infection induced StICS1 transcription.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- P. infestans infectione0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- StICS1e0
- Outcome
- StICS1 transcriptione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StICS1 transcription was induced upon P. infestans infection”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
The pathogen-responsive StICS1 expression was reduced in StbZIP61 RNAi plants.
- Taxon
- potatoSolanum tuberosume2
- Treatment
- StbZIP61 RNAi plantse0
- Interactor
- P. infestansPhytophthora infestanse1pathogen
- Gene
- StICS1e1
- Outcome
- responsive expressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this responsive expression to the pathogen was reduced in StbZIP61 RNAi plants”
e1“StICS1 transcription was induced upon P. infestans infection”
e2“potato (Solanum tuberosum)”
Claim · model-written summary
StICS1 expression was enhanced in StbZIP61-overexpressing plants.
- Taxon
- potatoSolanum tuberosume1
- Treatment
- StbZIP61-overexpressing plantse0
- Gene
- StICS1e0
- Outcome
- StICS1 expressione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StICS1 expression was remarkably enhanced in StbZIP61-overexpressing plants”
e1“potato (Solanum tuberosum)”
Claim · model-written summary
StbZIP61 and StNPR3L regulate temporal activation of SA biosynthesis that contributes to potato immunity.
- Taxon
- potatoSolanum tuberosume0
- Interactor
- P. infestansPhytophthora infestanse0pathogen
- Gene
- StbZIP61e0
- Outcome
- SA-mediated immunitye0
- Qualifier
- temporal activation of SA biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“StbZIP61 functions in concert with StNPR3L to regulate the temporal activation of SA biosynthesis, which contributes to SA-mediated immunity against P. infestans infection in potato”
The same questions in other papers
ethene · molecular: expression · Solanum tuberosum (potato)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
1 paper more in this group
See all 5 papers →salicylic acid · molecular: expression · Solanum tuberosum (potato)Positive and negative results
14 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
12 papers more in this group
See all 16 papers →jasmonic acid · molecular: expression · Solanum tuberosum (potato)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Phytophthora infestans · molecular: expression · Solanum tuberosum (potato)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →infection · molecular: expression · Solanum tuberosum (potato)Positive and negative results
16 papers in the same direction as this paper · 4 in the opposite direction
- positive
- negative
- negative
17 papers more in this group
See all 21 papers →loss of function · Phytophthora infestans · Solanum tuberosum (potato)Positive and negative results
14 papers in the same direction as this paper · 16 in the opposite direction
- positive
- positive
- positive
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.