Abstract
Aged tuber slices of potato cultivars Rishiri (R1-gene) and Irish cobbler (r-gene) were inoculated with race 0 (incompatible with Rishiri and compatible with Irish cobbler) and race 1 (compatible with both cultivars) of Phytophthora infestans. After inoculation, they were treated, at intervals, with 3H-leucine, H332PO4 and 86RbCl solution for 50min, and then washed and homogenized. Uptake of 3H-leucine, and 32P into the 2×104×g supernatant fraction of the homogenates of the slices (Rishiri and Irish cobbler) infected by both races were already lower than those of noninfected ones 1.4hr after inoculation, when most of encysted zoospores had germinated but not yet penetrated the host-cell wall. From about 2.4hr after inoculation, when about 20% of the appressoria had begun to penetrate, the rates of uptake of 3H-leucine and 32P begun to decrease more rapidly in the incompatible host-parasite combination than in those of compatible combinations: At this stage, no death of the infected cells has occurred yet even in the incompatible combination. In the case of 86Rb ion, on the contrary, the rate of uptake was higher in slices infected by the incompatible race than in non-infected slices or slices infected by the compatible race at 18C. At 10C, however there was little difference among the rates of uptake in the non-infected slices and slices infected by incompatible and compatible races.The most plausible explanation of these results is that the physiological activity of host-plasmalemma and/or the metabolic activity is altered by infection with the incompatible race in an early period of infection when no host cell death has occurred. From these results it was presumed that the alteration of physiological activity of plasmalemma and/or metabolism of host caused by recognition of infection by an incompatible race of P. infestans might occur almost simultaneously with penetration.
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Claims 5
Claim · model-written summary
Slices of Rishiri and Irish cobbler infected by race 0 or race 1 had lower uptake of 3H-leucine and 32P than noninfected slices 1.4 h after inoculation.
- Taxon
- potato
Solanum tuberosume0 - Material
- Rishiri (R1-gene) and Irish cobbler (r-gene)e0
- Organ / stage
- tuber slicese0
- Treatment
- inoculated with race 0e0
- Interactor
- Phytophthora infestans
Phytophthora infestanse0pathogen - Outcome
- Uptake of 3H-leucine, and 32Pe1
- Direction
- lowere1
- Qualifier
- 1.4hr after inoculatione1
- Comparator
- noninfected onese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aged tuber slices of potato cultivars Rishiri (R1-gene) and Irish cobbler (r-gene) were inoculated with race 0 (incompatible with Rishiri and compatible with Irish cobbler) and race 1 (compatible with both cultivars) of Phytophthora infestans”
e1“Uptake of 3H-leucine, and 32P into the 2×104×g supernatant fraction of the homogenates of the slices (Rishiri and Irish cobbler) infected by both races were already lower than those of noninfected ones 1.4hr after inoculation”
Claim · model-written summary
From 2.4 h after inoculation, uptake of 3H-leucine and 32P decreased more rapidly in the incompatible host-parasite combination than in compatible combinations.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- incompatible host-parasite combinatione2
- Interactor
- Phytophthora infestansPhytophthora infestanse0pathogen
- Outcome
- rates of uptake of 3H-leucine and 32Pe2
- Direction
- decreasee2
- Comparator
- compatible combinationse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aged tuber slices of potato cultivars Rishiri (R1-gene) and Irish cobbler (r-gene) were inoculated with race 0 (incompatible with Rishiri and compatible with Irish cobbler) and race 1 (compatible with both cultivars) of Phytophthora infestans”
e2“From about 2.4hr after inoculation, when about 20% of the appressoria had begun to penetrate, the rates of uptake of 3H-leucine and 32P begun to decrease more rapidly in the incompatible host-parasite combination than in those of compatible combinations”
Claim · model-written summary
Uptake of 86Rb was higher in slices infected by the incompatible race than in non-infected slices or slices infected by the compatible race at 18C.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- infected by the incompatible racee3
- Interactor
- Phytophthora infestansPhytophthora infestanse0pathogen
- Outcome
- rate of uptakee3
- Direction
- highere3
- Qualifier
- at 18Ce3
- Comparator
- non-infected slicese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aged tuber slices of potato cultivars Rishiri (R1-gene) and Irish cobbler (r-gene) were inoculated with race 0 (incompatible with Rishiri and compatible with Irish cobbler) and race 1 (compatible with both cultivars) of Phytophthora infestans”
e3“In the case of 86Rb ion, on the contrary, the rate of uptake was higher in slices infected by the incompatible race than in non-infected slices or slices infected by the compatible race at 18C”
Claim · model-written summary
At 10C, uptake rates of infected and non-infected slices showed little difference.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- infected by incompatible and compatible racese4
- Interactor
- Phytophthora infestansPhytophthora infestanse0pathogen
- Outcome
- rates of uptakee4
- Direction
- no_changeinferrede4
- Qualifier
- At 10Ce4
- Comparator
- non-infected slicese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aged tuber slices of potato cultivars Rishiri (R1-gene) and Irish cobbler (r-gene) were inoculated with race 0 (incompatible with Rishiri and compatible with Irish cobbler) and race 1 (compatible with both cultivars) of Phytophthora infestans”
e4“At 10C, however there was little difference among the rates of uptake in the non-infected slices and slices infected by incompatible and compatible races”
Claim · model-written summary
The alteration of host plasmalemma and metabolic activity by infection with the incompatible race is presumed to occur almost simultaneously with penetration.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- infection with the incompatible racee5
- Interactor
- Phytophthora infestansPhytophthora infestanse0pathogen
- Outcome
- physiological activity of host-plasmalemma and/or the metabolic activitye5
- Qualifier
- no host cell deathe5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Aged tuber slices of potato cultivars Rishiri (R1-gene) and Irish cobbler (r-gene) were inoculated with race 0 (incompatible with Rishiri and compatible with Irish cobbler) and race 1 (compatible with both cultivars) of Phytophthora infestans”
e5“The most plausible explanation of these results is that the physiological activity of host-plasmalemma and/or the metabolic activity is altered by infection with the incompatible race in an early period of infection when no host cell death has occurred”
The same questions in other papers
infection · Phytophthora infestans · Solanum tuberosum (potato)Positive and negative results
11 papers in the same direction as this paper · 15 in the opposite direction
- positive
- positive
- positive
23 papers more in this group
See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.