Paper · 2008 · Phytopathology

Quantification of Fusarium graminearum in Harvested Grain by Real-Time Polymerase Chain Reaction to Assess Efficacies of Fungicides on Fusarium Head Blight, Deoxynivalenol Contamination, and Yield of Winter Wheat

Y. J. Zhang; P. S. Fan; X. Zhang; et al.

12
claims
26
verbatim quotes
57
citations
2
research areas
—
Not open access

Abstract

We used a real time polymerase chain reaction-based assay and visual disease assessment to evaluate the efficacies of Js399-19, tebuconazole, a mixture of tebuconazole and thiram, azoxystrobin, carbendazim, and thiram on the development of Fusarium head blight (FHB) and deoxynivalenol (DON) contamination and on the yield of winter wheat (cv. Nannong no. 9918) after artificial inoculation under field conditions with Fusarium graminearum. The incidence of infected spikelets (IIS), amount of F. graminearum DNA (Tri5 DNA), total DON (containing DON, 3-acetyl-deoxynivalenol, and 15-acetyl-deoxynivalenol) concentration, and 1,000-grain weight (TGW) were quantified in 2006 and 2007. A strong positive correlation was found between IIS or Log10Tri5 DNA and total DON concentration in the harvested grain. The Js399-19, tebuconazole, and the mixture of tebuconazole and thiram significantly reduced IIS of FHB, amount of Tri5 DNA, and total DON within the grain and increased TGW. Although azoxystrobin, carbendazim, and thiram can increase TGW, they had no effect on the occurrence of F. graminearum compared with those of the untreated controls. Surprisingly, azoxystrobin and carbendazim significantly increased the total DON content in the harvested grain because they might have stimulated the amount of total DON production per Tri5 DNA. The fungicides Js399-19, tebuconazole, and the mixture of tebuconazole and thiram were the most effective in controlling FHB and reducing DON contamination of the wheat.

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Claims 12

Associationobservedoriginal resultmeasured

Claim · model-written summary

Infected spikelet incidence was positively correlated with grain DON concentration.

Taxon
Winter WheatTriticum aestivumt0
Interactor
Fusarium graminearumFusarium graminearumt1pathogene0
Outcome
total DON concentratione0
Direction
positive correlatione0
Qualifier
IISe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Fusarium graminearum
Fusarium graminearum · InteractorScot Nelson from Honolulu, USA · CC0 · Wikimedia Commons ↗

Verbatim

e0

“A strong positive correlation was found between IIS or Log10Tri5 DNA and total DON concentration in the harvested grain”

✓ located verbatim in the source · abstract, characters 685–804 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
t1

“Fusarium graminearum”

✓ located verbatim in the source · title, characters 18–38 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Fungal Tri5 DNA was positively correlated with grain DON concentration.

Taxon
Winter WheatTriticum aestivumt0
Interactor
Fusarium graminearumFusarium graminearumt1pathogene0
Outcome
total DON concentratione0
Direction
positive correlatione0
Qualifier
Log10Tri5 DNAe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Fusarium graminearum
Fusarium graminearum · InteractorScot Nelson from Honolulu, USA · CC0 · Wikimedia Commons ↗

Verbatim

e0

“A strong positive correlation was found between IIS or Log10Tri5 DNA and total DON concentration in the harvested grain”

✓ located verbatim in the source · abstract, characters 685–804 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
t1

“Fusarium graminearum”

✓ located verbatim in the source · title, characters 18–38 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Js399-19 reduced infected spikelet incidence, fungal DNA and grain DON and increased grain weight.

Taxon
Winter WheatTriticum aestivumt0
Treatment
Js399-19e0
Outcome
IIS of FHB, amount of Tri5 DNA, and total DON within the graine0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The Js399-19, tebuconazole, and the mixture of tebuconazole and thiram significantly reduced IIS of FHB, amount of Tri5 DNA, and total DON within the grain and increased TGW”

✓ located verbatim in the source · abstract, characters 806–979 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Tebuconazole reduced disease incidence, fungal DNA and grain DON.

Taxon
Winter WheatTriticum aestivumt0
Treatment
tebuconazolee0
Outcome
IIS of FHB, amount of Tri5 DNA, and total DON within the graine0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“tebuconazole, and the mixture of tebuconazole and thiram significantly reduced IIS of FHB, amount of Tri5 DNA, and total DON within the grain”

✓ located verbatim in the source · abstract, characters 820–961 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The tebuconazole and thiram mixture reduced disease incidence, fungal DNA and grain DON.

Taxon
Winter WheatTriticum aestivumt0
Treatment
mixture of tebuconazole and thirame0
Outcome
IIS of FHB, amount of Tri5 DNA, and total DON within the graine0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the mixture of tebuconazole and thiram significantly reduced IIS of FHB, amount of Tri5 DNA, and total DON within the grain”

✓ located verbatim in the source · abstract, characters 838–961 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Js399-19, tebuconazole and their stated mixture increased thousand grain weight.

Taxon
Winter WheatTriticum aestivumt0
Treatment
Js399-19e0
Outcome
TGWe0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The Js399-19, tebuconazole, and the mixture of tebuconazole and thiram significantly reduced IIS of FHB, amount of Tri5 DNA, and total DON within the grain and increased TGW”

✓ located verbatim in the source · abstract, characters 806–979 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Azoxystrobin increased thousand grain weight.

Taxon
Winter WheatTriticum aestivumt0
Treatment
azoxystrobine0
Outcome
TGWe0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“azoxystrobin, carbendazim, and thiram can increase TGW”

✓ located verbatim in the source · abstract, characters 990–1044 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Carbendazim increased thousand grain weight.

Taxon
Winter WheatTriticum aestivumt0
Treatment
carbendazime0
Outcome
TGWe0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“azoxystrobin, carbendazim, and thiram can increase TGW”

✓ located verbatim in the source · abstract, characters 990–1044 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Thiram increased thousand grain weight.

Taxon
Winter WheatTriticum aestivumt0
Treatment
thirame0
Outcome
TGWe0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“azoxystrobin, carbendazim, and thiram can increase TGW”

✓ located verbatim in the source · abstract, characters 990–1044 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Azoxystrobin, carbendazim and thiram had no effect on Fusarium occurrence relative to untreated controls.

Taxon
Winter WheatTriticum aestivumt0
Interactor
F. graminearumFusarium graminearume0pathogen
Outcome
occurrence of F. graminearume0
Direction
no effecte0
Comparator
untreated controlse0

Not reportedTreatment

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Fusarium graminearum
Fusarium graminearum · InteractorScot Nelson from Honolulu, USA · CC0 · Wikimedia Commons ↗

Verbatim

e0

“they had no effect on the occurrence of F. graminearum compared with those of the untreated controls”

✓ located verbatim in the source · abstract, characters 1046–1146 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Azoxystrobin significantly increased grain DON content.

Taxon
Winter WheatTriticum aestivumt0
Treatment
azoxystrobine0
Outcome
total DON contente0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“azoxystrobin and carbendazim significantly increased the total DON content in the harvested grain”

✓ located verbatim in the source · abstract, characters 1162–1259 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑
Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Carbendazim significantly increased grain DON content.

Taxon
Winter WheatTriticum aestivumt0
Treatment
carbendazime0
Outcome
total DON contente0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“azoxystrobin and carbendazim significantly increased the total DON content in the harvested grain”

✓ located verbatim in the source · abstract, characters 1162–1259 · show in text ↑
t0

“Winter Wheat”

✓ located verbatim in the source · title, characters 200–212 · show in text ↑

azoxystrobin · disease · Triticum aestivum (wheat)Positive and negative results

17 papers: 12 positive, 5 negative or no effect

4 papers in the same direction as this paper · 12 in the opposite direction

  1. positive
  2. positive
  3. no effect

13 papers more in this group

See all 17 papers →

carbendazim · disease · Triticum aestivum (wheat)Positive and negative results

20 papers: 15 positive, 5 negative or no effect

4 papers in the same direction as this paper · 15 in the opposite direction

  1. negative
  2. positive
  3. positive

16 papers more in this group

See all 20 papers →

thiram · disease · Triticum aestivum (wheat)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

tebuconazole · disease · Triticum aestivum (wheat)Results agree

36 papers: 36 positive, 0 negative or no effect

35 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

32 papers more in this group

See all 36 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.