Abstract
The optimized in vitro culture system was investigated for improvement of regeneration efficiencies by observing the responses of scutella-derived callus of Korean rice (Oryza sativa L.). Large variations of callus induction (43.9-93.9%) and shoot regeneration (0-88.7%) were observed among the rice cultivars depending on medium. However, shoot regeneration was significantly improved by selected utilization of basal medium, growth regulators, and carbon sources. N6 basal medium was more efficient for embryogenic callus induction than MS or LS basal medium, while MS was superior to N6 for shoot regeneration. The calli of highly regenerative cultivars grew faster and showed higher rates of green tissue formation (GT) and shoot regeneration (SR) and lower rate of callus browning (CB) than those of recalcitrant cultivars. Although a higher level of kinetin stimulated the GT and SR in highly regenerative cultivars, 10 ㎎L?¹ kinetin generally suppressed the GT and SR, while CB was accelerated compared to 2 ㎎L?¹ kinetin. Additional benefits of sorbitol combined with maltose (or sucrose) under 5 ㎎L?¹ kinetin were certainly confirmed on regeneration efficiencies compared to sucrose alone as carbon source and osmotic regulator. This combination showed high rate of GT and SR with multiple shoots while low rate of CB. With MSRK5SM-Pr medium (5 ㎎L?¹ kinetin, 3% sorbitol, 2% maltose, 500 ㎎L?¹ proline), the regeneration efficiencies of total 17 out of 24 cultivars were practically improved 160% on average compared to MSRK2S (2 ㎎L?¹ kinetin, 3% sucrose) control medium. Especially, the medium was most effective to the cultivars showing a medium level of regenerability such as Daesanbyeo and Dongjinbyeo and Suwon477, enhancing efficiencies more than 300-600% compared to MSRK2S medium.
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Claims 9
Claim · model-written summary
Callus induction and shoot regeneration varied among cultivars and media.
- Taxon
- rice
Oryza sativae0 - Outcome
- callus inductione0
- Value
- 43.9-93.9%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Large variations of callus induction (43.9-93.9%) and shoot regeneration (0-88.7%) were observed among the rice cultivars depending on medium.”
Claim · model-written summary
Shoot regeneration ranged from 0 to 88.7%.
- Taxon
- riceOryza sativae0
- Outcome
- shoot regeneratione0
- Value
- 0-88.7%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“shoot regeneration (0-88.7%) were observed among the rice cultivars depending on medium”
Claim · model-written summary
N6 was more efficient for embryogenic callus induction than MS or LS.
- Taxon
- RiceOryza sativat0
- Treatment
- N6 basal mediume0
- Outcome
- embryogenic callus inductione0
- Direction
- more efficiente0
- Comparator
- MS or LS basal mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N6 basal medium was more efficient for embryogenic callus induction than MS or LS basal medium”
t0“Rice”
Claim · model-written summary
MS surpassed N6 for shoot regeneration.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS was superior to N6 for shoot regeneration”
t0“Rice”
Claim · model-written summary
Highly regenerative cultivars grew faster and had more green tissue and shoot regeneration but less browning.
- Taxon
- RiceOryza sativat0
- Material
- highly regenerative cultivarse0
- Outcome
- green tissue formation (GT) and shoot regeneration (SR)e0
- Direction
- highere0
- Comparator
- recalcitrant cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The calli of highly regenerative cultivars grew faster and showed higher rates of green tissue formation (GT) and shoot regeneration (SR) and lower rate of callus browning (CB) than those of recalcitrant cultivars.”
t0“Rice”
Claim · model-written summary
At 10 ㎎L?¹ kinetin, green tissue and shoot regeneration were suppressed and browning accelerated versus 2 ㎎L?¹.
- Taxon
- RiceOryza sativat0
- Treatment
- 10 ㎎L?¹ kinetine0
- Outcome
- GT and SRe0
- Direction
- suppressede0
- Comparator
- 2 ㎎L?¹ kinetine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“10 ㎎L?¹ kinetin generally suppressed the GT and SR, while CB was accelerated compared to 2 ㎎L?¹ kinetin”
t0“Rice”
Claim · model-written summary
Sorbitol with maltose or sucrose improved regeneration relative to sucrose alone.
- Taxon
- RiceOryza sativat0
- Treatment
- sorbitol combined with maltose (or sucrose) under 5 ㎎L?¹ kinetine0
- Outcome
- regeneration efficienciese0
- Direction
- Additional benefitse0
- Comparator
- sucrose alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Additional benefits of sorbitol combined with maltose (or sucrose) under 5 ㎎L?¹ kinetin were certainly confirmed on regeneration efficiencies compared to sucrose alone as carbon source and osmotic regulator”
t0“Rice”
Claim · model-written summary
MSRK5SM-Pr improved regeneration in 17 of 24 cultivars by 160% on average.
- Taxon
- RiceOryza sativat0
- Treatment
- MSRK5SM-Pr mediume0
- Outcome
- regeneration efficienciese0
- Direction
- improvede0
- Value
- 160%e0
- Comparator
- MSRK2Se0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With MSRK5SM-Pr medium (5 ㎎L?¹ kinetin, 3% sorbitol, 2% maltose, 500 ㎎L?¹ proline), the regeneration efficiencies of total 17 out of 24 cultivars were practically improved 160% on average compared to MSRK2S (2 ㎎L?¹ kinetin, 3% sucrose) control medium.”
t0“Rice”
Claim · model-written summary
The medium enhanced regeneration more than 300-600% for medium-regenerability cultivars.
- Taxon
- RiceOryza sativat0
- Treatment
- the mediume0
- Outcome
- efficienciese0
- Direction
- enhancinge0
- Value
- more than 300-600%e0
- Comparator
- MSRK2S mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the medium was most effective to the cultivars showing a medium level of regenerability such as Daesanbyeo and Dongjinbyeo and Suwon477, enhancing efficiencies more than 300-600% compared to MSRK2S medium”
t0“Rice”
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See all 165 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.