Abstract
This study investigated the influence of osmotic stress, induced by sorbitol and sucrose combinations, on growth and proline accumulation in callus cultures of rice (Oryza sativa L.). Dehusked mature seeds, cv. Hassawi, were induced to callus on MS medium supplemented with 4.52 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.32 µM 6-furfurylaminopurine (kinetin). The medium also contained 29.2, 58.4, 87.6, and 116.8 mM sucrose combined with 0, 54.9, 109.8, and 164.7 mM sorbitol. Callus formation was observed in about 35 % of the cultured seeds irrespective of the sugar treatment. An increase in callus mass was observed as sucrose concentration increased reaching a maximum growth at 87.6 mM. Callus growth was enhanced in response to 54.9 mM sorbitol but at higher concentration it was inhibitory. Best callus growth was obtained on a medium containing 54.9 mM sorbitol combined with 87.6 mM sucrose. Increasing osmotic stress, as a consequence of increasing sucrose and sorbitol concentrations, induced proline accumulation and the highest concentration of proline, 5.8 µmol g-1(f.m.), was obtained on 164.7 mM sorbitol combined with 116.8 mM sucrose.
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Claims 7
Claim · model-written summary
Callus formed in about 35% of cultured seeds regardless of sugar treatment.
- Taxon
- rice
Oryza sativat0 - Outcome
- Callus formatione0
- Value
- 35 %e0
- Qualifier
- irrespective of the sugar treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus formation was observed in about 35 % of the cultured seeds irrespective of the sugar treatment”
t0“Rice”
Claim · model-written summary
Callus mass increased with sucrose concentration, peaking at 87.6 mM.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An increase in callus mass was observed as sucrose concentration increased reaching a maximum growth at 87.6 mM”
t0“Rice”
Claim · model-written summary
Sorbitol at 54.9 mM enhanced callus growth.
- Taxon
- riceOryza sativat0
- Treatment
- 54.9 mM sorbitole0
- Outcome
- Callus growthe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus growth was enhanced in response to 54.9 mM sorbitol”
t0“Rice”
Claim · model-written summary
Higher sorbitol concentrations inhibited callus growth.
- Taxon
- riceOryza sativat0
- Treatment
- higher concentratione0
- Outcome
- Callus growthe0
- Direction
- inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus growth was enhanced in response to 54.9 mM sorbitol but at higher concentration it was inhibitory”
t0“Rice”
Claim · model-written summary
Best callus growth occurred with 54.9 mM sorbitol and 87.6 mM sucrose.
- Taxon
- riceOryza sativat0
- Treatment
- 54.9 mM sorbitol combined with 87.6 mM sucrosee0
- Outcome
- Best callus growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Best callus growth was obtained on a medium containing 54.9 mM sorbitol combined with 87.6 mM sucrose”
t0“Rice”
Claim · model-written summary
Increasing sucrose and sorbitol osmotic stress induced proline accumulation.
- Taxon
- riceOryza sativat0
- Treatment
- Increasing osmotic stresse0
- Outcome
- proline accumulatione0
- Direction
- Increasinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing osmotic stress, as a consequence of increasing sucrose and sorbitol concentrations, induced proline accumulation”
t0“Rice”
Claim · model-written summary
Highest proline concentration was 5.8 µmol g-1(f.m.) at 164.7 mM sorbitol plus 116.8 mM sucrose.
- Taxon
- riceOryza sativat0
- Treatment
- 164.7 mM sorbitol combined with 116.8 mM sucrosee0
- Outcome
- highest concentration of prolinee0
- Value
- 5.8 µmol g-1(f.m.)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the highest concentration of proline, 5.8 µmol g-1(f.m.), was obtained on 164.7 mM sorbitol combined with 116.8 mM sucrose”
t0“Rice”
The same questions in other papers
glucitol · growth · Oryza sativa (rice)Results agree
8 papers in the same direction as this paper
- positive
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.