Abstract
Shoot branching is regulated by multiple signals. Previous studies have indicated that sucrose may promote shoot branching through suppressing the inhibitory effect of the hormone strigolactone (SL). However, the molecular mechanisms underlying this effect are unknown. Here, we used molecular and genetic tools to identify the molecular targets underlying the antagonistic interaction between sucrose and SL. We showed that sucrose antagonizes the suppressive action of SL on tillering in rice and on the degradation of D53, a major target of SL signalling. Sucrose inhibits the gene expression of D3, the orthologue of the Arabidopsis F-box MAX2 required for SL signalling. Overexpression of D3 antagonizes sucrose inhibition of D53 degradation and enables the SL inhibition of tillering under high sucrose. Sucrose prevents SL-induced degradation of D14, the SL receptor involved in D53 degradation. In contrast to D3, D14 overexpression enhances D53 protein levels and sucrose-induced tillering, even in the presence of SL. Our results show that sucrose inhibits SL response by affecting key components of SL signalling and, together with previous studies reporting the inhibition of SL synthesis by nitrate and phosphate, demonstrate the central role played by SLs in the regulation of plant architecture by nutrients.
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Claims 9
Claim · model-written summary
Sucrose antagonized SL suppression of rice tillering.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose antagonizes the suppressive action of SL on tillering in rice”
Claim · model-written summary
Sucrose antagonized SL promotion of D53 degradation.
- Taxon
- rice
Oryza sativat0 - Treatment
- sucrosee0
- Gene
- D53e0
- Outcome
- degradation of D53e0
- Direction
- antagonizese0
- Comparator
- SLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose antagonizes the suppressive action of SL on tillering in rice and on the degradation of D53”
t0“rice”
Claim · model-written summary
Sucrose inhibited D3 gene expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose inhibits the gene expression of D3”
t0“rice”
Claim · model-written summary
D3 overexpression countered sucrose inhibition of D53 degradation.
- Taxon
- riceOryza sativat0
- Treatment
- Overexpression of D3e0
- Gene
- D3e0
- Outcome
- D53 degradatione0
- Direction
- antagonizese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of D3 antagonizes sucrose inhibition of D53 degradation”
t0“rice”
Claim · model-written summary
D3 overexpression allowed SL to inhibit tillering despite high sucrose.
- Taxon
- riceOryza sativat0
- Treatment
- Overexpression of D3e0
- Gene
- D3e0
- Outcome
- tilleringe0
- Direction
- inhibitione0
- Qualifier
- under high sucrosee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of D3 antagonizes sucrose inhibition of D53 degradation and enables the SL inhibition of tillering under high sucrose”
t0“rice”
Claim · model-written summary
Sucrose prevented SL-induced D14 degradation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose prevents SL-induced degradation of D14”
t0“rice”
Claim · model-written summary
D14 overexpression enhanced D53 protein levels even with SL.
- Taxon
- riceOryza sativat0
- Treatment
- D14 overexpressione0
- Gene
- D14e0
- Outcome
- D53 protein levelse0
- Direction
- enhancese0
- Qualifier
- even in the presence of SLe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“D14 overexpression enhances D53 protein levels and sucrose-induced tillering, even in the presence of SL”
t0“rice”
Claim · model-written summary
D14 overexpression enhanced sucrose-induced tillering even with SL.
- Taxon
- riceOryza sativat0
- Treatment
- D14 overexpressione0
- Gene
- D14e0
- Outcome
- sucrose-induced tilleringe0
- Direction
- enhancese0
- Qualifier
- even in the presence of SLe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“D14 overexpression enhances D53 protein levels and sucrose-induced tillering, even in the presence of SL”
t0“rice”
Claim · model-written summary
The authors infer that sucrose inhibits SL response through key SL signaling components.
- Taxon
- riceOryza sativat0
- Treatment
- sucrosee0
- Outcome
- SL responsee0
- Qualifier
- affecting key components of SL signallinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sucrose inhibits SL response by affecting key components of SL signalling”
t0“rice”
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See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.