Abstract
This study aimed to clarify the possible mechanism of endogenous phytohormone signaling and carbohydrate metabolism during shoot organogenesis induced by osmotic stress in rice ( Oryza sativa L. cv. Tainung 71) callus. Non-regenerable calli derived from Tainung 71 immature embryos were inoculated on Murashige and Skoog medium containing 10 μM 2,4-D. They turned to highly regenerable calli (HRC) (regeneration frequency more than 75 %) with lower calli fresh weight and water content when 0.6 M sorbitol was supplemented into the medium. The regeneration frequency was prominently decreased to 25 % while an auxin transport inhibitor, 2,3,5-triiodobenzoic acid (TIBA), was added into the sorbitol-treated medium. It suggested that endogenous auxin signal may be involved in the induction of HRC under osmotic stress treatment. As well, HRC showed high levels of glucose, sucrose, and starch and high expression of cell wall - bound invertase 1 , sucrose transporter 1 ( OsSUT1 ), OsSUT2 , PIN - formed 1 , and late embryogenesis abundant 1 ( OsLEA1 ) genes. Their expressions are all dramatic inhibited except OsLEA1 under TIBA treatment. It suggests a key role of auxin may be linked to the effect of shoot regeneration under osmotic stress treatment. Therefore, we present a putative hypothesis for regenerable calli induction by osmotic stress treatment in rice. Osmotic stress may regulate endogenous levels of auxin interacting with abscisic acid, then affect carbohydrate metabolism to trigger callus initiation and further shoot regeneration in rice.
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Claims 8
Claim · model-written summary
Sorbitol osmotic stress increased the regeneration frequency of rice callus to more than 75 percent
- Taxon
- Oryza sativa
Oryza sativae1 - Material
- Tainung 71e1
- Treatment
- 0.6 M sorbitole2
- Outcome
- regeneration frequencye2
- Direction
- increaseinferrede2
- Value
- more than 75 %e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Oryza sativa L. cv. Tainung 71”
e2“They turned to highly regenerable calli (HRC) (regeneration frequency more than 75 %) with lower calli fresh weight and water content when 0.6 M sorbitol was supplemented into the medium”
Claim · model-written summary
Sorbitol osmotic stress lowered calli fresh weight and water content in rice callus
- Taxon
- Oryza sativaOryza sativae1
- Material
- Tainung 71e1
- Treatment
- 0.6 M sorbitole2
- Outcome
- calli fresh weight and water contente2
- Direction
- lowere2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Oryza sativa L. cv. Tainung 71”
e2“They turned to highly regenerable calli (HRC) (regeneration frequency more than 75 %) with lower calli fresh weight and water content when 0.6 M sorbitol was supplemented into the medium”
Claim · model-written summary
TIBA, an auxin transport inhibitor, decreased the regeneration frequency of sorbitol-treated rice callus to 25 percent
- Taxon
- Oryza sativaOryza sativae1
- Treatment
- TIBAe3
- Outcome
- regeneration frequencye3
- Direction
- decreasede3
- Value
- 25 %e3
- Comparator
- sorbitol-treated mediume3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Oryza sativa L. cv. Tainung 71”
e3“The regeneration frequency was prominently decreased to 25 % while an auxin transport inhibitor, 2,3,5-triiodobenzoic acid (TIBA), was added into the sorbitol-treated medium”
Claim · model-written summary
Highly regenerable rice callus showed high levels of glucose, sucrose and starch
- Taxon
- Oryza sativaOryza sativae1
- Outcome
- levels of glucose, sucrose, and starche4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Oryza sativa L. cv. Tainung 71”
e4“HRC showed high levels of glucose, sucrose, and starch”
Claim · model-written summary
Highly regenerable rice callus showed high expression of cell wall-bound invertase 1 and sucrose transporter OsSUT1
- Taxon
- Oryza sativaOryza sativae1
- Gene
- OsSUT1e6
- Outcome
- high expressione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Oryza sativa L. cv. Tainung 71”
e5“high expression of cell wall - bound invertase 1”
e6“sucrose transporter 1 ( OsSUT1 )”
Claim · model-written summary
TIBA treatment inhibited the expression of carbohydrate and auxin-related genes in highly regenerable rice callus
- Taxon
- Oryza sativaOryza sativae1
- Treatment
- TIBA treatmente2
- Outcome
- expressionse2
- Direction
- dramatic inhibitede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Oryza sativa L. cv. Tainung 71”
e2“Their expressions are all dramatic inhibited except OsLEA1 under TIBA treatment”
Claim · model-written summary
OsLEA1 expression was not inhibited under TIBA treatment in rice callus
- Taxon
- Oryza sativaOryza sativae1
- Treatment
- TIBA treatmente2
- Gene
- OsLEA1e2
- Outcome
- expressionse2
- Direction
- no_changeinferrede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Oryza sativa L. cv. Tainung 71”
e2“Their expressions are all dramatic inhibited except OsLEA1 under TIBA treatment”
Claim · model-written summary
Osmotic stress may regulate auxin levels interacting with abscisic acid and affect carbohydrate metabolism to trigger callus initiation and shoot regeneration in rice
- Taxon
- riceOryza sativae7
- Compound
- auxine7
- Outcome
- callus initiation and further shoot regeneratione7
- Qualifier
- auxin interacting with abscisic acide7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Oryza sativa L. cv. Tainung 71”
e7“Osmotic stress may regulate endogenous levels of auxin interacting with abscisic acid, then affect carbohydrate metabolism to trigger callus initiation and further shoot regeneration in rice”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.