Paper · 2025 · Russian Journal of Plant Physiology

Impact of Humic Acid on Leaf Carbohydrate Metabolism in Timely and Late Sown Varieties of Wheat (Triticum aestivum L.)

A. Rawal; N. Johal; A. Kaur; et al.

14
claims
29
verbatim quotes
0
citations
2
research areas
—
Not open access

Abstract

Abstract The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown) were sown in randomised block design in PVC cylinders and field conditions at PAU and Bathinda Punjab India. Foliar application of two concentration of humic acid (2 and 4 ppm) was done ten days prior to tillering, booting and flowering stage. The treatment without any spray served as control. At all stages morpho-physiological and biochemical parameters were recorded whereas yield and yield attributes were recorded at maturity. The present study found that foliar spray of 2 and 4 ppm humic acid significantly increased plant height in flowering and grain filling stages. The booting stage exhibited the maximum dry weight (0.089 g) in PBW 752 at 4 ppm dosage. The 4 ppm foliar spray significantly increased the relative growth rate of PBW 725 (15%) at Bathinda. Humic acid reduced membrane damage in PBW 725 and PBW 752 plants at Bathinda, and increased cellular viability (72%) with 4 ppm foliar spray. The chlorophyll a (25.84 µg/g FW), chlorophyll b (10.96 µg/g FW), carotenoids (10.46 µg/g FW), total chlorophyll content (33.5 µg/g FW) along with leaf relative water content (99%) spiked after 4 ppm foliar spray of humic acid. The flowering stage showed an unprecedented value of total soluble sugars for PBW 725 (23.6%) on 4 ppm treatment of humic acid. The study found that starch content (12.9%) increased significantly PBW 752 at Bathinda after 4 ppm of foliar spray of humic acid. The highest α-amylase activity (133.1 nmol of glucose formed 1/min g FW) was observed in PBW 752 at booting stage after 4 ppm of foliar spray of humic acid. On the other hand, foliar spray of humic acid did not depict any significant effect on yield components. Thus, α-amylase activity was augmented by 4 ppm of humic acid at booting stage, which improved the levels of photosynthates, which in turn improved yield attributes.

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Claims 14

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Foliar humic acid at 2 and 4 ppm significantly increased wheat plant height at flowering and grain filling stages.

Organ / stage
flowering and grain filling stagese1
Treatment
foliar spray of 2 and 4 ppm humic acide1
Outcome
plant heighte1
Direction
increasede1
Comparator
treatment without any spraye2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e1

“foliar spray of 2 and 4 ppm humic acid significantly increased plant height in flowering and grain filling stages”

✓ located verbatim in the source · abstract, characters 570–683 · show in text ↑
e2

“The treatment without any spray served as control”

✓ located verbatim in the source · abstract, characters 352–401 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The booting stage of PBW 752 wheat at 4 ppm humic acid showed the maximum dry weight, 0.089 g.

Material
PBW 752e3
Organ / stage
booting stagee3
Treatment
4 ppm dosagee3
Outcome
dry weighte3
Direction
maximume3
Value
0.089 ge3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e3

“The booting stage exhibited the maximum dry weight (0.089 g) in PBW 752 at 4 ppm dosage”

✓ located verbatim in the source · abstract, characters 685–772 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Foliar 4 ppm humic acid significantly increased the relative growth rate of PBW 725 wheat at Bathinda by 15%.

Material
PBW 725e4
Treatment
4 ppm foliar spraye4
Outcome
relative growth ratee4
Direction
increasede4
Value
15%e4
Setting
Bathindae4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e4

“The 4 ppm foliar spray significantly increased the relative growth rate of PBW 725 (15%) at Bathinda”

✓ located verbatim in the source · abstract, characters 774–874 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Humic acid reduced membrane damage in PBW 725 and PBW 752 wheat at Bathinda.

Material
PBW 725 and PBW 752e5
Treatment
Humic acide5
Outcome
membrane damagee5
Direction
reducede5
Setting
Bathindae5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e5

“Humic acid reduced membrane damage in PBW 725 and PBW 752 plants at Bathinda”

✓ located verbatim in the source · abstract, characters 876–952 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar 4 ppm humic acid increased cellular viability of wheat by 72%.

Treatment
4 ppm foliar spraye6
Outcome
cellular viabilitye6
Direction
increasede6
Value
72%e6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e6

“increased cellular viability (72%) with 4 ppm foliar spray”

✓ located verbatim in the source · abstract, characters 958–1016 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar 4 ppm humic acid spiked wheat chlorophyll a content to 25.84 µg/g FW.

Treatment
4 ppm foliar spray of humic acide7
Outcome
chlorophyll ae7
Direction
spikede7
Value
25.84 µg/g FWe7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e7

“chlorophyll a (25.84 µg/g FW), chlorophyll b (10.96 µg/g FW), carotenoids (10.46 µg/g FW), total chlorophyll content (33.5 µg/g FW) along with leaf relative water content (99%) spiked after 4 ppm foliar spray of humic acid”

✓ located verbatim in the source · abstract, characters 1022–1244 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar 4 ppm humic acid spiked wheat chlorophyll b content to 10.96 µg/g FW.

Treatment
4 ppm foliar spray of humic acide7
Outcome
chlorophyll be7
Direction
spikede7
Value
10.96 µg/g FWe7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e7

“chlorophyll a (25.84 µg/g FW), chlorophyll b (10.96 µg/g FW), carotenoids (10.46 µg/g FW), total chlorophyll content (33.5 µg/g FW) along with leaf relative water content (99%) spiked after 4 ppm foliar spray of humic acid”

✓ located verbatim in the source · abstract, characters 1022–1244 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar 4 ppm humic acid spiked wheat carotenoid content to 10.46 µg/g FW.

Treatment
4 ppm foliar spray of humic acide7
Outcome
carotenoidse7
Direction
spikede7
Value
10.46 µg/g FWe7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e7

“chlorophyll a (25.84 µg/g FW), chlorophyll b (10.96 µg/g FW), carotenoids (10.46 µg/g FW), total chlorophyll content (33.5 µg/g FW) along with leaf relative water content (99%) spiked after 4 ppm foliar spray of humic acid”

✓ located verbatim in the source · abstract, characters 1022–1244 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar 4 ppm humic acid spiked total chlorophyll content of wheat to 33.5 µg/g FW.

Treatment
4 ppm foliar spray of humic acide7
Outcome
total chlorophyll contente7
Direction
spikede7
Value
33.5 µg/g FWe7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e7

“chlorophyll a (25.84 µg/g FW), chlorophyll b (10.96 µg/g FW), carotenoids (10.46 µg/g FW), total chlorophyll content (33.5 µg/g FW) along with leaf relative water content (99%) spiked after 4 ppm foliar spray of humic acid”

✓ located verbatim in the source · abstract, characters 1022–1244 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar 4 ppm humic acid spiked leaf relative water content of wheat to 99%.

Treatment
4 ppm foliar spray of humic acide7
Outcome
leaf relative water contente7
Direction
spikede7
Value
99%e7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e7

“chlorophyll a (25.84 µg/g FW), chlorophyll b (10.96 µg/g FW), carotenoids (10.46 µg/g FW), total chlorophyll content (33.5 µg/g FW) along with leaf relative water content (99%) spiked after 4 ppm foliar spray of humic acid”

✓ located verbatim in the source · abstract, characters 1022–1244 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar 4 ppm humic acid at the flowering stage gave an unprecedented total soluble sugar value of 23.6% in PBW 725 wheat.

Material
PBW 725e1
Organ / stage
flowering stagee1
Treatment
4 ppm treatment of humic acide1
Outcome
total soluble sugarse1
Direction
unprecedented valuee1
Value
23.6%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e1

“The flowering stage showed an unprecedented value of total soluble sugars for PBW 725 (23.6%) on 4 ppm treatment of humic acid”

✓ located verbatim in the source · abstract, characters 1246–1372 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Foliar 4 ppm humic acid significantly increased starch content of PBW 752 wheat at Bathinda to 12.9%.

Material
PBW 752e1
Treatment
4 ppm of foliar spray of humic acide1
Outcome
starch contente1
Direction
increasede1
Value
12.9%e1
Setting
Bathindae1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e1

“The study found that starch content (12.9%) increased significantly PBW 752 at Bathinda after 4 ppm of foliar spray of humic acid”

✓ located verbatim in the source · abstract, characters 1374–1503 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar 4 ppm humic acid gave the highest alpha-amylase activity in PBW 752 wheat at the booting stage.

Material
PBW 752e1
Organ / stage
booting stagee1
Treatment
4 ppm of foliar spray of humic acide1
Outcome
α-amylase activitye1
Direction
higheste1
Value
133.1 nmol of glucose formed 1/min g FWe1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e1

“The highest α-amylase activity (133.1 nmol of glucose formed 1/min g FW) was observed in PBW 752 at booting stage after 4 ppm of foliar spray of humic acid”

✓ located verbatim in the source · abstract, characters 1505–1660 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Foliar humic acid did not have any significant effect on wheat yield components.

Treatment
foliar spray of humic acide1
Outcome
yield componentse1
Direction
did not depict any significant effecte1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The present study investigated wheat varieties i.e., PBW 725 (timely sown) and PBW 752 (late sown)”

✓ located verbatim in the source · abstract, characters 9–107 · show in text ↑
e1

“foliar spray of humic acid did not depict any significant effect on yield components”

✓ located verbatim in the source · abstract, characters 1681–1765 · show in text ↑

other · physiology: osmolytes · Triticum aestivum (wheat)Positive and negative results

8 papers: 7 positive, 1 negative or no effect

6 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

4 papers more in this group

See all 8 papers →

other · physiology: water · Triticum aestivum (wheat)Positive and negative results

17 papers: 16 positive, 1 negative or no effect

15 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

13 papers more in this group

See all 17 papers →

application · quality: sugar starch · Triticum aestivum (wheat)Positive and negative results

21 papers: 19 positive, 2 negative or no effect

18 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

17 papers more in this group

See all 21 papers →

application · physiology: water · Triticum aestivum (wheat)Positive and negative results

51 papers: 49 positive, 2 negative or no effect

48 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

47 papers more in this group

See all 51 papers →

application · physiology: pigments · Triticum aestivum (wheat)Positive and negative results

106 papers: 98 positive, 8 negative or no effect

97 papers in the same direction as this paper · 6 in the opposite direction · 2 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

102 papers more in this group

See all 106 papers →

other · growth · Triticum aestivum (wheat)Positive and negative results

114 papers: 100 positive, 14 negative or no effect

99 papers in the same direction as this paper · 14 in the opposite direction

  1. positive
  2. positive
  3. no effect

110 papers more in this group

See all 114 papers →

other · yield · Triticum aestivum (wheat)Positive and negative results

223 papers: 206 positive, 17 negative or no effect

16 papers in the same direction as this paper · 206 in the opposite direction

  1. positive
  2. no effect
  3. positive

219 papers more in this group

See all 223 papers →

application · growth · Triticum aestivum (wheat)Positive and negative results

307 papers: 273 positive, 34 negative or no effect

272 papers in the same direction as this paper · 32 in the opposite direction · 2 reporting no difference from an alternative

  1. positive
  2. no effect
  3. no effect

303 papers more in this group

See all 307 papers →

8 of 12 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.