Abstract
Alfalfa is an excellent leguminous forage crop that is widely cultivated worldwide, but its yield and quality are often affected by drought and soil salinization. Hyperosmolality-gated calcium-permeable channel (OSCA) proteins are hyperosmotic calcium ion (Ca 2+ ) receptors that play an essential role in regulating plant growth, development, and abiotic stress responses. However, no systematic analysis of the OSCA gene family has been conducted in alfalfa. In this study, a total of 14 OSCA genes were identified from the alfalfa genome and classified into three groups based on their sequence composition and phylogenetic relationships. Gene structure, conserved motifs and functional domain prediction showed that all MsOSCA genes had the same functional domain DUF221. Cis -acting element analysis showed that MsOSCA genes had many cis -regulatory elements in response to abiotic or biotic stresses and hormones. Tissue expression pattern analysis demonstrated that the MsOSCA genes had tissue-specific expression; for example, MsOSCA12 was only expressed in roots and leaves but not in stem and petiole tissues. Furthermore, RT–qPCR results indicated that the expression of MsOSCA genes was induced by abiotic stress (drought and salt) and hormones (JA, SA, and ABA). In particular, the expression levels of MsOSCA3 , MsOSCA5 , MsOSCA12 and MsOSCA13 were significantly increased under drought and salt stress, and MsOSCA7 , MsOSCA10 , MsOSCA12 and MsOSCA13 genes exhibited significant upregulation under plant hormone treatments, indicating that these genes play a positive role in drought, salt and hormone responses. Subcellular localization results showed that the MsOSCA3 protein was localized on the plasma membrane. This study provides a basis for understanding the biological information and further functional analysis of the MsOSCA gene family and provides candidate genes for stress resistance breeding in alfalfa.
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Claims 16
Claim · model-written summary
Fourteen OSCA genes were identified in alfalfa and assigned to three groups.
- Taxon
- alfalfa
Medicago sativae0 - Gene
- OSCA genese0
- Outcome
- identifiede0
- Value
- 14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 14 OSCA genes were identified from the alfalfa genome and classified into three groups”
Claim · model-written summary
All MsOSCA genes had the DUF221 functional domain.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsOSCA genese0
- Outcome
- functional domain DUF221e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all MsOSCA genes had the same functional domain DUF221”
t0“alfalfa”
Claim · model-written summary
MsOSCA genes had cis-regulatory elements responsive to abiotic or biotic stresses and hormones.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsOSCA genese0
- Outcome
- cis -regulatory elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsOSCA genes had many cis -regulatory elements in response to abiotic or biotic stresses and hormones”
t0“alfalfa”
Claim · model-written summary
MsOSCA genes showed tissue-specific expression.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsOSCA genese0
- Outcome
- tissue-specific expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsOSCA genes had tissue-specific expression”
t0“alfalfa”
Claim · model-written summary
MsOSCA12 expression occurred in roots and leaves but not stems or petioles.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsOSCA12e0
- Outcome
- expressed in roots and leaves but not in stem and petiole tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsOSCA12 was only expressed in roots and leaves but not in stem and petiole tissues”
t0“alfalfa”
Claim · model-written summary
Drought and salt stress induced MsOSCA gene expression.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- drought and salte0
- Gene
- MsOSCA genese0
- Outcome
- expression of MsOSCA genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of MsOSCA genes was induced by abiotic stress (drought and salt)”
t0“alfalfa”
Claim · model-written summary
JA, SA and ABA treatments induced MsOSCA expression.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- JA, SA, and ABAe0
- Gene
- MsOSCA genese0
- Outcome
- expression of MsOSCA genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of MsOSCA genes was induced by abiotic stress (drought and salt) and hormones (JA, SA, and ABA)”
t0“alfalfa”
Claim · model-written summary
MsOSCA3 expression significantly increased under drought and salt stress.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- drought and salt stresse0
- Gene
- MsOSCA3e0
- Outcome
- expression levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of MsOSCA3 , MsOSCA5 , MsOSCA12 and MsOSCA13 were significantly increased under drought and salt stress”
t0“alfalfa”
Claim · model-written summary
MsOSCA5 expression significantly increased under drought and salt stress.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- drought and salt stresse0
- Gene
- MsOSCA5e0
- Outcome
- expression levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of MsOSCA3 , MsOSCA5 , MsOSCA12 and MsOSCA13 were significantly increased under drought and salt stress”
t0“alfalfa”
Claim · model-written summary
MsOSCA12 expression significantly increased under drought and salt stress.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- drought and salt stresse0
- Gene
- MsOSCA12e0
- Outcome
- expression levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of MsOSCA3 , MsOSCA5 , MsOSCA12 and MsOSCA13 were significantly increased under drought and salt stress”
t0“alfalfa”
Claim · model-written summary
MsOSCA13 expression significantly increased under drought and salt stress.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- drought and salt stresse0
- Gene
- MsOSCA13e0
- Outcome
- expression levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of MsOSCA3 , MsOSCA5 , MsOSCA12 and MsOSCA13 were significantly increased under drought and salt stress”
t0“alfalfa”
Claim · model-written summary
MsOSCA7 was significantly upregulated by plant hormone treatments.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- plant hormone treatmentse0
- Gene
- MsOSCA7e0
- Outcome
- upregulatione0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsOSCA7 , MsOSCA10 , MsOSCA12 and MsOSCA13 genes exhibited significant upregulation under plant hormone treatments”
t0“alfalfa”
Claim · model-written summary
MsOSCA10 was significantly upregulated by plant hormone treatments.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- plant hormone treatmentse0
- Gene
- MsOSCA10e0
- Outcome
- upregulatione0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsOSCA7 , MsOSCA10 , MsOSCA12 and MsOSCA13 genes exhibited significant upregulation under plant hormone treatments”
t0“alfalfa”
Claim · model-written summary
MsOSCA12 was significantly upregulated by plant hormone treatments.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- plant hormone treatmentse0
- Gene
- MsOSCA12e0
- Outcome
- upregulatione0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsOSCA7 , MsOSCA10 , MsOSCA12 and MsOSCA13 genes exhibited significant upregulation under plant hormone treatments”
t0“alfalfa”
Claim · model-written summary
MsOSCA13 was significantly upregulated by plant hormone treatments.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- plant hormone treatmentse0
- Gene
- MsOSCA13e0
- Outcome
- upregulatione0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsOSCA7 , MsOSCA10 , MsOSCA12 and MsOSCA13 genes exhibited significant upregulation under plant hormone treatments”
t0“alfalfa”
Claim · model-written summary
MsOSCA3 protein localized to the plasma membrane.
- Taxon
- alfalfaMedicago sativat0
- Gene
- MsOSCA3e0
- Outcome
- localized on the plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsOSCA3 protein was localized on the plasma membrane”
t0“alfalfa”
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