Abstract
myo-Inositol phosphate synthase (MIPS) is the key enzyme of myo-inositol synthesis, which is a central molecule required for cell metabolism and plant growth as a precursor to a large variety of compounds. A full-length fragment of MfMIPS1 cDNA was cloned from Medicago falcata that is more cold-tolerant than Medicago sativa. While MfMIPS1 transcript was induced in response to cold, dehydration and salt stress, MIPS transcript and myo-inositol were maintained longer and at a higher level in M. falcata than in M. sativa during cold acclimation at 5 °C. MfMIPS1 transcript was induced by hydrogen peroxide (H(2) O(2)) and nitric oxide (NO), but was not responsive to abscisic acid (ABA). Pharmacological experiments revealed that H(2) O(2) and NO are involved in the regulation of MfMIPS1 expression by cold and dehydration, but not by salt. Overexpression of MfMIPS1 in tobacco increased the MIPS activity and levels of myo-inositol, galactinol and raffinose, resulting in enhanced resistance to chilling, drought and salt stresses in transgenic tobacco plants. It is suggested that MfMIPS1 is induced by diverse environmental factors and confers resistance to various abiotic stresses.
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Claims 17
Claim · model-written summary
MfMIPS1 cDNA was cloned from Medicago falcata.
- Taxon
- Medicago falcata
Medicago sativa subsp. falcatae0 - Gene
- MfMIPS1e0
- Outcome
- MfMIPS1 cDNA was clonede0
- Value
- full-length fragmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A full-length fragment of MfMIPS1 cDNA was cloned from Medicago falcata”
Claim · model-written summary
Medicago falcata was more cold tolerant than Medicago sativa.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae0
- Outcome
- cold-tolerante0
- Direction
- moree0
- Comparator
- Medicago sativae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Medicago falcata that is more cold-tolerant than Medicago sativa”
Claim · model-written summary
Cold induced MfMIPS1 transcript in Medicago falcata.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Treatment
- colde0
- Gene
- MfMIPS1e0
- Outcome
- MfMIPS1 transcripte0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MfMIPS1 transcript was induced in response to cold”
e1“Medicago falcata”
Claim · model-written summary
Dehydration induced MfMIPS1 transcript in Medicago falcata.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Treatment
- dehydratione0
- Gene
- MfMIPS1e0
- Outcome
- MfMIPS1 transcripte0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MfMIPS1 transcript was induced in response to cold, dehydration and salt stress”
e1“Medicago falcata”
Claim · model-written summary
Salt stress induced MfMIPS1 transcript in Medicago falcata.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Treatment
- salt stresse0
- Gene
- MfMIPS1e0
- Outcome
- MfMIPS1 transcripte0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MfMIPS1 transcript was induced in response to cold, dehydration and salt stress”
e1“Medicago falcata”
Claim · model-written summary
During cold acclimation, MIPS transcript in Medicago falcata remained longer and higher than in Medicago sativa.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Treatment
- cold acclimation at 5 °Ce0
- Gene
- MIPSe0
- Outcome
- MIPS transcripte0
- Direction
- highere0
- Qualifier
- maintained longere0
- Comparator
- M. sativae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MIPS transcript and myo-inositol were maintained longer and at a higher level in M. falcata than in M. sativa during cold acclimation at 5 °C”
e1“Medicago falcata”
Claim · model-written summary
During cold acclimation, myo-inositol in Medicago falcata remained longer and higher than in Medicago sativa.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Treatment
- cold acclimation at 5 °Ce0
- Outcome
- myo-inositole0
- Direction
- highere0
- Qualifier
- maintained longere0
- Comparator
- M. sativae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MIPS transcript and myo-inositol were maintained longer and at a higher level in M. falcata than in M. sativa during cold acclimation at 5 °C”
e1“Medicago falcata”
Claim · model-written summary
Hydrogen peroxide induced MfMIPS1 transcript in Medicago falcata.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Treatment
- hydrogen peroxide (H(2) O(2))e0
- Gene
- MfMIPS1e0
- Outcome
- MfMIPS1 transcripte0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MfMIPS1 transcript was induced by hydrogen peroxide (H(2) O(2)) and nitric oxide (NO)”
e1“Medicago falcata”
Claim · model-written summary
Nitric oxide induced MfMIPS1 transcript in Medicago falcata.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Treatment
- nitric oxide (NO)e0
- Gene
- MfMIPS1e0
- Outcome
- MfMIPS1 transcripte0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MfMIPS1 transcript was induced by hydrogen peroxide (H(2) O(2)) and nitric oxide (NO)”
e1“Medicago falcata”
Claim · model-written summary
MfMIPS1 transcript did not respond to abscisic acid in Medicago falcata.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae2
- Treatment
- abscisic acid (ABA)e0
- Gene
- MfMIPS1e1
- Outcome
- MfMIPS1 transcripte1
- Direction
- not responsivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was not responsive to abscisic acid (ABA)”
e1“MfMIPS1 transcript was induced by hydrogen peroxide”
e2“Medicago falcata”
Claim · model-written summary
Hydrogen peroxide regulated MfMIPS1 expression under cold and dehydration, but not salt, in Medicago falcata.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Gene
- MfMIPS1e0
- Compound
- H(2) O(2)e0
- Outcome
- regulation of MfMIPS1 expressione0
- Qualifier
- by cold and dehydration, but not by salte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H(2) O(2) and NO are involved in the regulation of MfMIPS1 expression by cold and dehydration, but not by salt”
e1“Medicago falcata”
Claim · model-written summary
Nitric oxide regulated MfMIPS1 expression under cold and dehydration, but not salt, in Medicago falcata.
- Taxon
- Medicago falcataMedicago sativa subsp. falcatae1
- Gene
- MfMIPS1e0
- Compound
- NOe0
- Outcome
- regulation of MfMIPS1 expressione0
- Qualifier
- by cold and dehydration, but not by salte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“H(2) O(2) and NO are involved in the regulation of MfMIPS1 expression by cold and dehydration, but not by salt”
e1“Medicago falcata”
Claim · model-written summary
MfMIPS1 overexpression in tobacco increased MIPS activity.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Overexpression of MfMIPS1e0
- Gene
- MfMIPS1e0
- Outcome
- MIPS activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of MfMIPS1 in tobacco increased the MIPS activity and levels of myo-inositol, galactinol and raffinose”
Claim · model-written summary
MfMIPS1 overexpression in tobacco increased myo-inositol, galactinol and raffinose levels.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of MfMIPS1e0
- Gene
- MfMIPS1e0
- Outcome
- levels of myo-inositol, galactinol and raffinosee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of MfMIPS1 in tobacco increased the MIPS activity and levels of myo-inositol, galactinol and raffinose”
Claim · model-written summary
MfMIPS1 overexpression enhanced tobacco resistance to chilling.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of MfMIPS1e0
- Gene
- MfMIPS1e0
- Outcome
- resistance to chillinge0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of MfMIPS1 in tobacco increased the MIPS activity and levels of myo-inositol, galactinol and raffinose, resulting in enhanced resistance to chilling, drought and salt stresses”
Claim · model-written summary
MfMIPS1 overexpression enhanced tobacco resistance to drought.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of MfMIPS1e0
- Gene
- MfMIPS1e0
- Outcome
- resistance to chilling, drought and salt stressese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of MfMIPS1 in tobacco increased the MIPS activity and levels of myo-inositol, galactinol and raffinose, resulting in enhanced resistance to chilling, drought and salt stresses”
Claim · model-written summary
MfMIPS1 overexpression enhanced tobacco resistance to salt stress.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of MfMIPS1e0
- Gene
- MfMIPS1e0
- Outcome
- resistance to chilling, drought and salt stressese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of MfMIPS1 in tobacco increased the MIPS activity and levels of myo-inositol, galactinol and raffinose, resulting in enhanced resistance to chilling, drought and salt stresses”
The same questions in other papers
hydrogen peroxide · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- positive
- no effect
(+)-abscisic acid · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
4 papers in the same direction as this paper · 13 in the opposite direction
- positive
- positive
- positive
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See all 18 papers →nitric oxide · molecular: expression · Medicago sativa (alfalfa)Results agree
2 papers in the same direction as this paper
- positive
- positive
cold · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
7 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →salinity · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
8 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- negative
- negative
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See all 15 papers →drought · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
13 papers in the same direction as this paper · 7 in the opposite direction
- positive
- no effect
- no effect
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.