Abstract
Cyclins are key regulators of the cell cycle in all eukaryotes. We have previously isolated two B-type cyclin genes, cycMs1 and cycMs2, from alfalfa that are primarily expressed during the G2-to-M phase transition and are most likely mitotic cyclin genes. Here, we report the isolation of a novel alfalfa cyclin gene, termed cycMs3 (for cyclin Medicago sativa), by selecting for mating type alpha-pheromone-induced cell cycle arrest suppression in yeast. The central region of the predicted amino acid sequence of the cycMs3 gene is most similar to the cyclin box of yeast B-type and mammalian A- and B-type cyclins. In situ hybridization showed that cycMs3 mRNA can be detected only in proliferating cells and not in differentiated alfalfa cells. When differentiated G0-arrested cells were induced to reenter the cell cycle in the G1 phase and resume cell division by treatment with plant hormones, cycMs3 transcript levels increased long before the onset of DNA synthesis. In contrast, histone H3-1 mRNA and cycMs2 transcripts were not observed before DNA replication and mitosis, respectively. In addition, cycMs3 mRNA was found in all stages of the cell cycle in synchronously dividing cells, whereas the cycMs2 and histone H3-1 genes showed a G2-to-M phase- or S phase-specific transcription pattern, respectively. These data suggest that the role of cyclin CycMs3 differs from that of CycMs1 and CycMs2. We propose that CycMs3 helps control reentry of quiescent G0-arrested cells into the G1 phase of the cell cycle.
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Claims 10
Claim · model-written summary
A novel alfalfa cyclin gene cycMs3 was isolated.
- Taxon
- alfalfa
Medicago sativae0 - Gene
- cycMs3e0
- Outcome
- isolation of a novel alfalfa cyclin genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we report the isolation of a novel alfalfa cyclin gene, termed cycMs3”
Claim · model-written summary
The central predicted CycMs3 sequence resembled yeast B-type and mammalian A- and B-type cyclin boxes.
- Taxon
- alfalfaMedicago sativat0
- Gene
- cycMs3 genee0
- Outcome
- most similar to the cyclin boxe0
- Comparator
- yeast B-type and mammalian A- and B-type cyclinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The central region of the predicted amino acid sequence of the cycMs3 gene is most similar to the cyclin box of yeast B-type and mammalian A- and B-type cyclins.”
t0“alfalfa”
Claim · model-written summary
cycMs3 mRNA was detected only in proliferating cells.
- Taxon
- alfalfaMedicago sativae0
- Gene
- cycMs3e0
- Outcome
- mRNA can be detected only in proliferating cellse0
- Comparator
- differentiated alfalfa cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cycMs3 mRNA can be detected only in proliferating cells and not in differentiated alfalfa cells.”
Claim · model-written summary
Hormone-induced cell-cycle reentry increased cycMs3 transcripts before DNA synthesis.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- treatment with plant hormonese0
- Gene
- cycMs3e0
- Outcome
- cycMs3 transcript levelse0
- Direction
- increasede0
- Qualifier
- long before the onset of DNA synthesise0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When differentiated G0-arrested cells were induced to reenter the cell cycle in the G1 phase and resume cell division by treatment with plant hormones, cycMs3 transcript levels increased long before the onset of DNA synthesis.”
t0“alfalfa”
Claim · model-written summary
Histone H3-1 mRNA was absent before DNA replication.
- Taxon
- alfalfaMedicago sativat0
- Gene
- histone H3-1e0
- Outcome
- histone H3-1 mRNAe0
- Qualifier
- before DNA replicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“histone H3-1 mRNA and cycMs2 transcripts were not observed before DNA replication and mitosis, respectively.”
t0“alfalfa”
Claim · model-written summary
cycMs2 transcripts were absent before mitosis.
- Taxon
- alfalfaMedicago sativat0
- Gene
- cycMs2e0
- Outcome
- cycMs2 transcriptse0
- Qualifier
- before DNA replication and mitosis, respectivelye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“histone H3-1 mRNA and cycMs2 transcripts were not observed before DNA replication and mitosis, respectively.”
t0“alfalfa”
Claim · model-written summary
cycMs3 mRNA appeared at all cell-cycle stages in synchronously dividing cells.
- Taxon
- alfalfaMedicago sativat0
- Gene
- cycMs3e0
- Outcome
- mRNA was found in all stages of the cell cyclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cycMs3 mRNA was found in all stages of the cell cycle in synchronously dividing cells”
t0“alfalfa”
Claim · model-written summary
cycMs2 transcription was specific to G2-to-M.
- Taxon
- alfalfaMedicago sativat0
- Gene
- cycMs2e0
- Outcome
- G2-to-M phase- or S phase-specific transcription patterne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cycMs2 and histone H3-1 genes showed a G2-to-M phase- or S phase-specific transcription pattern, respectively.”
t0“alfalfa”
Claim · model-written summary
Histone H3-1 transcription was specific to S phase.
- Taxon
- alfalfaMedicago sativat0
- Gene
- histone H3-1e0
- Outcome
- G2-to-M phase- or S phase-specific transcription patterne0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cycMs2 and histone H3-1 genes showed a G2-to-M phase- or S phase-specific transcription pattern, respectively.”
t0“alfalfa”
Claim · model-written summary
Authors propose CycMs3 helps control reentry of quiescent cells into G1.
- Taxon
- alfalfaMedicago sativat0
- Gene
- CycMs3e0
- Outcome
- reentry of quiescent G0-arrested cells into the G1 phasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We propose that CycMs3 helps control reentry of quiescent G0-arrested cells into the G1 phase of the cell cycle.”
t0“alfalfa”
The same questions in other papers
plant hormone · molecular: expression · Medicago sativa (alfalfa)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.