Abstract
Infiltration of Medicago sativa leaves with a suspension of Pseudomonas syringae pv. pisi elicits the accumulation of several mRNA classes. A clone, designated as MsPR10-1, encoding a polypeptide exhibiting strong similarity to the class 10 PR protein was isolated and characterized from a cDNA library prepared from leaf mRNA. The corresponding gene was shown to be developmentally regulated: Except in roots, its expression was not detectable in other analyzed organs of healthy plants (hypocotyls, cotyledons, stems, leaves, and flower buds). MsPR10-1 transcript accumulation was especially high in leaf blades during an incompatible interaction: It was already detectable 3 h after infection, reached its maximum level 24 h postinfection, and remained at a high level over a period of at least 72 h. In addition, the expression of this gene was induced by salicylic acid treatment of the leaves. Southern hybridizations showed that this gene belongs to a multigene family. Using a 5' extension technique for cDNA, we demonstrated that during the incompatible interaction with P. syringae pv. pisi several genes or allelic variants of this class were expressed. Measurements of transcript accumulation in both the infiltrated and noninfiltrated zones by Northern and in situ hybridization allowed to demonstrate the "systemic" expression pattern of the MsPR10-1. In situ hybridizations indicated that MsPR10-1 was expressed in the vascular bundles adjacent to and distant from the infection site.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Pseudomonas infiltration elicited multiple mRNA classes.
- Taxon
- Medicago sativa
Medicago sativae0 - Treatment
- Infiltration of Medicago sativa leaves with a suspension of Pseudomonas syringae pv. pisie0
- Interactor
- Pseudomonas syringae pv. pisi
Pseudomonas syringae pv. pisie0pathogen - Outcome
- accumulation of several mRNA classese0
- Direction
- elicitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infiltration of Medicago sativa leaves with a suspension of Pseudomonas syringae pv. pisi elicits the accumulation of several mRNA classes.”
Claim · model-written summary
MsPR10-1 encodes a class 10 PR-like polypeptide.
- Taxon
- Medicago sativaMedicago sativat0
- Gene
- MsPR10-1e0
- Outcome
- polypeptide exhibiting strong similarity to the class 10 PR proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A clone, designated as MsPR10-1, encoding a polypeptide exhibiting strong similarity to the class 10 PR protein was isolated and characterized”
t0“Medicago sativa”
Claim · model-written summary
In healthy plants, expression was detectable in roots but not the other analyzed organs.
- Taxon
- Medicago sativaMedicago sativat0
- Organ / stage
- hypocotyls, cotyledons, stems, leaves, and flower budse0
- Outcome
- expression was not detectable in other analyzed organse0
- Qualifier
- Except in rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Except in roots, its expression was not detectable in other analyzed organs of healthy plants (hypocotyls, cotyledons, stems, leaves, and flower buds).”
t0“Medicago sativa”
Claim · model-written summary
MsPR10-1 transcript accumulated strongly in leaf blades during incompatible infection, from 3 to at least 72 hours, peaking at 24 hours.
- Taxon
- Medicago sativaMedicago sativat0
- Organ / stage
- leaf bladese0
- Gene
- MsPR10-1e0
- Outcome
- transcript accumulatione0
- Direction
- especially highe0
- Value
- 24 he0
- Qualifier
- over a period of at least 72 he0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsPR10-1 transcript accumulation was especially high in leaf blades during an incompatible interaction: It was already detectable 3 h after infection, reached its maximum level 24 h postinfection, and remained at a high level over a period of at least 72 h.”
t0“Medicago sativa”
Claim · model-written summary
Salicylic acid induced expression of the gene in leaves.
- Taxon
- Medicago sativaMedicago sativat0
- Organ / stage
- leavese0
- Treatment
- salicylic acid treatmente0
- Outcome
- expression of this genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of this gene was induced by salicylic acid treatment of the leaves.”
t0“Medicago sativa”
Claim · model-written summary
Southern hybridization placed the gene in a multigene family.
- Taxon
- Medicago sativaMedicago sativat0
- Outcome
- belongs to a multigene familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Southern hybridizations showed that this gene belongs to a multigene family.”
t0“Medicago sativa”
Claim · model-written summary
Several genes or allelic variants of this class were expressed during incompatible infection.
- Taxon
- Medicago sativaMedicago sativat0
- Interactor
- P. syringae pv. pisiPseudomonas syringae pv. pisie0pathogen
- Outcome
- several genes or allelic variants of this class were expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“during the incompatible interaction with P. syringae pv. pisi several genes or allelic variants of this class were expressed.”
t0“Medicago sativa”
Claim · model-written summary
MsPR10-1 was expressed systemically in vascular bundles near and far from the infection site.
- Taxon
- Medicago sativaMedicago sativat0
- Organ / stage
- vascular bundlese0
- Gene
- MsPR10-1e0
- Outcome
- expressed in the vascular bundles adjacent to and distant from the infection sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MsPR10-1 was expressed in the vascular bundles adjacent to and distant from the infection site.”
t0“Medicago sativa”
The same questions in other papers
salicylic acid · molecular: expression · Medicago sativa (alfalfa)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.