Abstract
P-HYDROXYPHENYLPYRUVATE DIOXYGENASE (HPPD) is the first committed enzyme involved in the biosynthesis of vitamin E, and is characterized by catalyzing the conversion of p-hydroxyphenyl pyruvate (HPP) to homogentisic acid (HGA). Here, an HPPD gene was cloned from Medicago sativa L. and designated MsHPPD, which was expressed at high levels in alfalfa leaves. PEG 6000 (polyethylene glycol), NaCl, abscisic acid and salicylic acid were shown to significantly induce MsHPPD expression, especially in the cotyledons and root tissues. Overexpression of MsHPPD was found to significantly increase the level of β-tocotrienol and the total vitamin E content in Arabidopsis seeds. Furthermore, these transgenic Arabidopsis seeds exhibited an accelerated germination time, compared with wild-type seeds under normal conditions, as well as under NaCl and ABA treatments. Meanwhile, the expression level of several genes associated with ABA biosynthesis (NCED3, NCED5 and NCED9) and the ABA signaling pathway (RAB18, ABI3 and ABI5) were significantly down-regulated in MsHPPD-overexpressing transgenic lines, as well as the total free ABA content. Taken together, these results demonstrate that MsHPPD functions not only in the vitamin E biosynthetic pathway, but also plays a critical role in seed germination via affecting ABA biosynthesis and signaling.
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Claims 15
Claim · model-written summary
An HPPD gene was cloned from Medicago sativa and designated MsHPPD.
- Taxon
- Medicago sativa L.
Medicago sativae0 - Gene
- MsHPPDe0
- Outcome
- HPPD gene was clonede0
Not reportedValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, an HPPD gene was cloned from Medicago sativa L. and designated MsHPPD, which was expressed at high levels in alfalfa leaves.”
Claim · model-written summary
MsHPPD was highly expressed in alfalfa leaves.
- Taxon
- Medicago sativa L.Medicago sativae0
- Organ / stage
- alfalfa leavese0
- Gene
- MsHPPDe0
- Outcome
- expressed at high levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, an HPPD gene was cloned from Medicago sativa L. and designated MsHPPD, which was expressed at high levels in alfalfa leaves.”
Claim · model-written summary
PEG 6000 significantly induced MsHPPD expression.
- Taxon
- Medicago sativaMedicago sativat0
- Organ / stage
- cotyledons and root tissuese0
- Treatment
- PEG 6000e0
- Gene
- MsHPPDe0
- Outcome
- MsHPPD expressione0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PEG 6000 (polyethylene glycol), NaCl, abscisic acid and salicylic acid were shown to significantly induce MsHPPD expression, especially in the cotyledons and root tissues.”
t0“P-HYDROXYPHENYLPYRUVATE DIOXYGENASE from Medicago sativa is involved in vitamin E biosynthesis and abscisic acid-mediated seed germination”
Claim · model-written summary
NaCl significantly induced MsHPPD expression.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- NaCle0
- Gene
- MsHPPDe0
- Outcome
- MsHPPD expressione0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PEG 6000 (polyethylene glycol), NaCl, abscisic acid and salicylic acid were shown to significantly induce MsHPPD expression, especially in the cotyledons and root tissues.”
t0“P-HYDROXYPHENYLPYRUVATE DIOXYGENASE from Medicago sativa is involved in vitamin E biosynthesis and abscisic acid-mediated seed germination”
Claim · model-written summary
Abscisic acid significantly induced MsHPPD expression.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- abscisic acide0
- Gene
- MsHPPDe0
- Outcome
- MsHPPD expressione0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PEG 6000 (polyethylene glycol), NaCl, abscisic acid and salicylic acid were shown to significantly induce MsHPPD expression, especially in the cotyledons and root tissues.”
t0“P-HYDROXYPHENYLPYRUVATE DIOXYGENASE from Medicago sativa is involved in vitamin E biosynthesis and abscisic acid-mediated seed germination”
Claim · model-written summary
Salicylic acid significantly induced MsHPPD expression.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- salicylic acide0
- Gene
- MsHPPDe0
- Outcome
- MsHPPD expressione0
- Direction
- inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PEG 6000 (polyethylene glycol), NaCl, abscisic acid and salicylic acid were shown to significantly induce MsHPPD expression, especially in the cotyledons and root tissues.”
t0“P-HYDROXYPHENYLPYRUVATE DIOXYGENASE from Medicago sativa is involved in vitamin E biosynthesis and abscisic acid-mediated seed germination”
Claim · model-written summary
MsHPPD overexpression significantly increased β-tocotrienol in Arabidopsis seeds.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- seedse0
- Treatment
- Overexpression of MsHPPDe0
- Gene
- MsHPPDe0
- Outcome
- level of β-tocotrienole0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of MsHPPD was found to significantly increase the level of β-tocotrienol and the total vitamin E content in Arabidopsis seeds.”
Claim · model-written summary
MsHPPD overexpression significantly increased total vitamin E content in Arabidopsis seeds.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- seedse0
- Treatment
- Overexpression of MsHPPDe0
- Gene
- MsHPPDe0
- Outcome
- total vitamin E contente0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of MsHPPD was found to significantly increase the level of β-tocotrienol and the total vitamin E content in Arabidopsis seeds.”
Claim · model-written summary
Transgenic Arabidopsis seeds germinated earlier than wild type under normal conditions.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- transgenic Arabidopsis seedse0
- Outcome
- germination timee0
- Direction
- acceleratede0
- Qualifier
- normal conditionse0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, these transgenic Arabidopsis seeds exhibited an accelerated germination time, compared with wild-type seeds under normal conditions, as well as under NaCl and ABA treatments.”
Claim · model-written summary
Transgenic Arabidopsis seeds germinated earlier than wild type under NaCl.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- transgenic Arabidopsis seedse0
- Outcome
- germination timee0
- Direction
- acceleratede0
- Qualifier
- NaCle0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, these transgenic Arabidopsis seeds exhibited an accelerated germination time, compared with wild-type seeds under normal conditions, as well as under NaCl and ABA treatments.”
Claim · model-written summary
Transgenic Arabidopsis seeds germinated earlier than wild type under ABA.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- transgenic Arabidopsis seedse0
- Outcome
- germination timee0
- Direction
- acceleratede0
- Qualifier
- ABAe0
- Comparator
- wild-type seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, these transgenic Arabidopsis seeds exhibited an accelerated germination time, compared with wild-type seeds under normal conditions, as well as under NaCl and ABA treatments.”
Claim · model-written summary
NCED3, NCED5 and NCED9 expression was significantly downregulated in MsHPPD-overexpressing lines.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- MsHPPD-overexpressing transgenic linese0
- Gene
- NCED3, NCED5 and NCED9e0
- Outcome
- expression levele0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Meanwhile, the expression level of several genes associated with ABA biosynthesis (NCED3, NCED5 and NCED9) and the ABA signaling pathway (RAB18, ABI3 and ABI5) were significantly down-regulated in MsHPPD-overexpressing transgenic lines, as well as the total free ABA content.”
t0“P-HYDROXYPHENYLPYRUVATE DIOXYGENASE from Medicago sativa is involved in vitamin E biosynthesis and abscisic acid-mediated seed germination”
Claim · model-written summary
RAB18, ABI3 and ABI5 expression was significantly downregulated in MsHPPD-overexpressing lines.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- MsHPPD-overexpressing transgenic linese0
- Gene
- RAB18, ABI3 and ABI5e0
- Outcome
- expression levele0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Meanwhile, the expression level of several genes associated with ABA biosynthesis (NCED3, NCED5 and NCED9) and the ABA signaling pathway (RAB18, ABI3 and ABI5) were significantly down-regulated in MsHPPD-overexpressing transgenic lines, as well as the total free ABA content.”
t0“P-HYDROXYPHENYLPYRUVATE DIOXYGENASE from Medicago sativa is involved in vitamin E biosynthesis and abscisic acid-mediated seed germination”
Claim · model-written summary
Total free ABA content was significantly downregulated in MsHPPD-overexpressing lines.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- MsHPPD-overexpressing transgenic linese0
- Outcome
- total free ABA contente0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Meanwhile, the expression level of several genes associated with ABA biosynthesis (NCED3, NCED5 and NCED9) and the ABA signaling pathway (RAB18, ABI3 and ABI5) were significantly down-regulated in MsHPPD-overexpressing transgenic lines, as well as the total free ABA content.”
t0“P-HYDROXYPHENYLPYRUVATE DIOXYGENASE from Medicago sativa is involved in vitamin E biosynthesis and abscisic acid-mediated seed germination”
Claim · model-written summary
Authors conclude MsHPPD functions in vitamin E biosynthesis and seed germination through ABA pathways.
- Taxon
- Medicago sativaMedicago sativat0
- Gene
- MsHPPDe0
- Outcome
- seed germinatione0
- Qualifier
- affecting ABA biosynthesis and signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Taken together, these results demonstrate that MsHPPD functions not only in the vitamin E biosynthetic pathway, but also plays a critical role in seed germination via affecting ABA biosynthesis and signaling.”
t0“P-HYDROXYPHENYLPYRUVATE DIOXYGENASE from Medicago sativa is involved in vitamin E biosynthesis and abscisic acid-mediated seed germination”
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.