Abstract
Cold-acclimation-specific (CAS) gene expression has been examined by screening a cDNA library prepared from poly(A)(+) RNA of cold-acclimated seedlings of a freezing-tolerant variety of alfalfa (Medicago falcata cv Anik). Three CAS cDNA clones, pSM784, pSM2201, and pSM2358, representing different sequence species, have been used to investigate the relative abundance and time-course of accumulation of corresponding transcripts. Results obtained show that the expression of these CAS genes is regulated in a coordinated manner most likely at the level of transcription. The expression of genes, as measured by mRNA abundance corresponding to the three CAS cDNA clones, is not stimulated or induced by heat shock, water stress, abscisic acid, or wounding. A positive correlation is observed between the expression of these cloned sequences and the degree of freezing-tolerance in four alfalfa cultivars.
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Claims 7
Claim · model-written summary
Three CAS cDNA clones represented different sequence species.
- Taxon
- alfalfa
Medicago sativat0 - Gene
- pSM784, pSM2201, and pSM2358e0
- Outcome
- different sequence speciese0
- Value
- Three CAS cDNA clonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three CAS cDNA clones, pSM784, pSM2201, and pSM2358, representing different sequence species”
t0“Alfalfa”
Claim · model-written summary
CAS gene expression was coordinated, most likely through transcriptional regulation.
- Taxon
- alfalfaMedicago sativat0
- Gene
- CAS genese0
- Outcome
- regulated in a coordinated mannere0
- Qualifier
- most likely at the level of transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of these CAS genes is regulated in a coordinated manner most likely at the level of transcription”
t0“Alfalfa”
Claim · model-written summary
Heat shock did not stimulate CAS transcript abundance.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- heat shocke0
- Gene
- CAS cDNA clonese0
- Outcome
- mRNA abundancee0
- Direction
- not stimulated or inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of genes, as measured by mRNA abundance corresponding to the three CAS cDNA clones, is not stimulated or induced by heat shock, water stress, abscisic acid, or wounding”
t0“Alfalfa”
Claim · model-written summary
Water stress did not stimulate CAS transcript abundance.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- water stresse0
- Gene
- CAS cDNA clonese0
- Outcome
- mRNA abundancee0
- Direction
- not stimulated or inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of genes, as measured by mRNA abundance corresponding to the three CAS cDNA clones, is not stimulated or induced by heat shock, water stress, abscisic acid, or wounding”
t0“Alfalfa”
Claim · model-written summary
Abscisic acid did not stimulate CAS transcript abundance.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- abscisic acide0
- Gene
- CAS cDNA clonese0
- Outcome
- mRNA abundancee0
- Direction
- not stimulated or inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of genes, as measured by mRNA abundance corresponding to the three CAS cDNA clones, is not stimulated or induced by heat shock, water stress, abscisic acid, or wounding”
t0“Alfalfa”
Claim · model-written summary
Wounding did not stimulate CAS transcript abundance.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- woundinge0
- Gene
- CAS cDNA clonese0
- Outcome
- mRNA abundancee0
- Direction
- not stimulated or inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of genes, as measured by mRNA abundance corresponding to the three CAS cDNA clones, is not stimulated or induced by heat shock, water stress, abscisic acid, or wounding”
t0“Alfalfa”
Claim · model-written summary
Cloned sequence expression correlated positively with freezing tolerance across four cultivars.
- Taxon
- alfalfaMedicago sativae0
- Material
- four alfalfa cultivarse0
- Gene
- cloned sequencese0
- Outcome
- degree of freezing-tolerancee0
- Direction
- positive correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A positive correlation is observed between the expression of these cloned sequences and the degree of freezing-tolerance in four alfalfa cultivars”
The same questions in other papers
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See all 7 papers →drought · molecular: expression · Medicago sativa (alfalfa)Positive and negative results
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See all 21 papers →heat · molecular: expression · Medicago sativa (alfalfa)Results agree
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- no effect
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.