Abstract
High-density planting aggravates competition among plants and has a negative impact on plant growth and productivity. Nitrogen application and chemical control can improve plant growth and increase grain yield in high-density planting. Our experiment explored the effects of nitrogen fertilizer and plant growth regulators on maize root-bleeding sap, phosphorus (P) and potassium (K) accumulation and translocation, and grain yield and quality in high-density planting. We established a field study during the 2017 and 2018 growing seasons, with three nitrogen levels of N100 (100 kg ha −1 ), N200 (200 kg ha −1 ), and N300 (300 kg ha −1 ) at high-density planting (90,000 plants ha −1 ), and applied Yuhuangjin (a plant growth regulator mixture of 3% DTA-6 and 27% ethephon) at the 7th leaf. Our results showed that N200 application combined with chemical control could regulate amino acid and mineral nutrient concentration delivery rates in root-bleeding sap and improve its sap rate. Also, the treated plant exhibited higher P and K uptake and translocation ability. Furthermore, chemical control and N200 treatment maintained a high level of ribulose-1,5-bisphosphate carboxylase (RuBPCase), phosphoenolpyruvate carboxylase (PEPCase), nitrate reductase (NR), and glutamine synthetase (GS) enzymatic activities in leaves. In addition, plant growth regulator and nitrogen application improved the enzymatic activities of GS, glutamate dehydrogenase (GDH), and glutamic pyruvic transaminase (GPT) and the contents of crude protein, lysine, sucrose, and soluble sugar in grain and ultimately increased maize yield. This study suggests that N200 application in combination with chemical control promotes root vitality and nutrient accumulation and could improve grain yield and quality in high-density planting.
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Claims 9
Claim · model-written summary
N200 with chemical control regulated amino acid and mineral nutrient delivery rates in root-bleeding sap.
- Treatment
- N200 application combined with chemical controle0
- Outcome
- amino acid and mineral nutrient concentration delivery rates in root-bleeding sape0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Maize”
e0“N200 application combined with chemical control could regulate amino acid and mineral nutrient concentration delivery rates in root-bleeding sap”
Claim · model-written summary
N200 with chemical control improved root-bleeding sap rate.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Maize”
e0“N200 application combined with chemical control could regulate amino acid and mineral nutrient concentration delivery rates in root-bleeding sap and improve its sap rate”
Claim · model-written summary
Treated maize had higher P and K uptake and translocation ability.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Maize”
e0“the treated plant exhibited higher P and K uptake and translocation ability”
Claim · model-written summary
Chemical control and N200 maintained high activities of four leaf enzymes.
- Organ / stage
- leavese0
- Treatment
- chemical control and N200 treatmente0
- Outcome
- ribulose-1,5-bisphosphate carboxylase (RuBPCase), phosphoenolpyruvate carboxylase (PEPCase), nitrate reductase (NR), and glutamine synthetase (GS) enzymatic activitiese0
- Direction
- maintained a high levele0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Maize”
e0“chemical control and N200 treatment maintained a high level of ribulose-1,5-bisphosphate carboxylase (RuBPCase), phosphoenolpyruvate carboxylase (PEPCase), nitrate reductase (NR), and glutamine synthetase (GS) enzymatic activities in leaves”
Claim · model-written summary
Growth regulator and nitrogen application improved grain GS, GDH and GPT activities.
- Treatment
- plant growth regulator and nitrogen applicatione0
- Outcome
- enzymatic activities of GS, glutamate dehydrogenase (GDH), and glutamic pyruvic transaminase (GPT)e0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Maize”
e0“plant growth regulator and nitrogen application improved the enzymatic activities of GS, glutamate dehydrogenase (GDH), and glutamic pyruvic transaminase (GPT)”
Claim · model-written summary
Growth regulator and nitrogen application improved crude protein and lysine contents in grain.
- Organ / stage
- graine0
- Treatment
- plant growth regulator and nitrogen applicatione0
- Outcome
- contents of crude protein, lysinee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Maize”
e0“plant growth regulator and nitrogen application improved the enzymatic activities of GS, glutamate dehydrogenase (GDH), and glutamic pyruvic transaminase (GPT) and the contents of crude protein, lysine, sucrose, and soluble sugar in grain”
Claim · model-written summary
Growth regulator and nitrogen application improved sucrose and soluble sugar contents in grain.
- Treatment
- plant growth regulator and nitrogen applicatione0
- Outcome
- sucrose, and soluble sugar in graine0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Maize”
e0“plant growth regulator and nitrogen application improved the enzymatic activities of GS, glutamate dehydrogenase (GDH), and glutamic pyruvic transaminase (GPT) and the contents of crude protein, lysine, sucrose, and soluble sugar in grain”
Claim · model-written summary
Growth regulator and nitrogen application increased maize yield.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“plant growth regulator and nitrogen application improved the enzymatic activities of GS, glutamate dehydrogenase (GDH), and glutamic pyruvic transaminase (GPT) and the contents of crude protein, lysine, sucrose, and soluble sugar in grain and ultimately increased maize yield”
Claim · model-written summary
The authors suggest N200 with chemical control to improve yield and quality in high-density maize planting.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Maize”
e0“N200 application in combination with chemical control promotes root vitality and nutrient accumulation and could improve grain yield and quality in high-density planting”
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