Abstract
Abstract Plant KRP proteins are cyclin/cyclin-dependent kinase subunit (Cdk) inhibitors that share a limited homology with mammalian p27 Kip1 proteins. Several KRPs have been reported in maize ( Zea mays L.), of which Zeama;KRP1 was studied during maize germination. Expression of the Zeama;KRP1 gene did not vary during the 24 h germination period. A homologous antibody raised against the 13 kDa carboxy end of the Zeama;KRP1 polypeptide, a sequence containing the cyclin/Cdk inhibitory domain, indicated the existence of a 22 kDa protein in maize embryonic axes, the amount of which also remained unchanged during germination. Neither abscisic acid nor cytokinins modified the amount of protein. The purified Zeama;KRP1 polypeptide inhibited the kinase activity associated with Zeama;PCNA and Zeama;CycD2;1, and also the kinase activity in p13 Suc1 -pulled-down complexes. However, there were differences in the inhibition pattern during germination. Whereas kinase activity in proliferating cell nuclear antigen (PCNA) or CycD2;1 immunoprecipitates was strongly inhibited mainly during early germination, that in p13 Suc1 -pulled-down complexes was mainly inhibited at late times, suggesting that each protein complex is composed of different cyclins and/or Cdks.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Zeama;KRP1 gene expression did not change during 24 hours of germination.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the Zeama;KRP1 gene did not vary during the 24 h germination period”
t0“maize”
Claim · model-written summary
A 22 kDa protein was detected in maize embryonic axes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicated the existence of a 22 kDa protein in maize embryonic axes”
t0“maize”
Claim · model-written summary
The protein amount remained unchanged during germination.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the amount of which also remained unchanged during germination”
t0“maize”
Claim · model-written summary
Abscisic acid did not change protein amount.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither abscisic acid nor cytokinins modified the amount of protein”
t0“maize”
Claim · model-written summary
Cytokinins did not change protein amount.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Neither abscisic acid nor cytokinins modified the amount of protein”
t0“maize”
Claim · model-written summary
Purified Zeama;KRP1 inhibited kinase activity associated with Zeama;PCNA and Zeama;CycD2;1.
- Treatment
- purified Zeama;KRP1 polypeptidee0
- Gene
- Zeama;KRP1e0
- Outcome
- kinase activity associated with Zeama;PCNA and Zeama;CycD2;1e0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified Zeama;KRP1 polypeptide inhibited the kinase activity associated with Zeama;PCNA and Zeama;CycD2;1”
t0“maize”
Claim · model-written summary
Purified Zeama;KRP1 inhibited kinase activity in p13 Suc1 complexes.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“also the kinase activity in p13 Suc1 -pulled-down complexes”
t0“maize”
e1“The purified Zeama;KRP1 polypeptide inhibited the kinase activity associated with Zeama;PCNA and Zeama;CycD2;1”
Claim · model-written summary
Inhibition of PCNA or CycD2;1 kinase activity was strongest early in germination.
- Organ / stage
- early germinatione0
- Outcome
- kinase activity in proliferating cell nuclear antigen (PCNA) or CycD2;1 immunoprecipitatese0
- Direction
- strongly inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“kinase activity in proliferating cell nuclear antigen (PCNA) or CycD2;1 immunoprecipitates was strongly inhibited mainly during early germination”
t0“maize”
Claim · model-written summary
Inhibition of p13 Suc1 complex kinase activity occurred mainly late in germination.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“that in p13 Suc1 -pulled-down complexes was mainly inhibited at late times”
t0“maize”
The same questions in other papers
class · quality: protein · Zea mays (maize)Positive and negative results
1 paper in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- positive
8 papers more in this group
See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.