Paper · 1975 · PLANT PHYSIOLOGY

Effect of Glucose on the Induction of Nitrate Reductase in Corn Roots

Muhammad Aslam; Ann Oaks

10
claims
20
verbatim quotes
75
citations
1
research areas
✓
Open access

Abstract

In Zea mays L., addition of glucose to the induction medium has no effect on the induction of nitrate reductase during the initial 3 hours either in root tips (0-10 mm) or mature root sections (25-35 mm). With longer times, higher levels of enzyme activity are recovered from both root segments when glucose is present in the incubation medium. The induction in root tips is saturated by 10 mm NO(3) (-). Higher concentrations of NO(3) (-) are required for saturation in mature root sections. The response to glucose is seen over a wide range of external NO(3) (-) concentrations.Nitrate reductase activity is lost rapidly when nitrate is withdrawn from the induction medium. Additions of glucose do not prevent this loss. Additions of glucose have no effect on total uptake of NO(3) (-) by the root segments but they increase the anaerobic NO(2) (-) production in both root tips and mature root segments. This latter measurement is considered to be an estimate of an active NO(3) (-) pool in the cytoplasm. Thus the results show that glucose alters the distribution of NO(3) (-) within the root sections. This may be an important factor in controlling the in vivo stability of the enzyme or its rate of synthesis.

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Claims 10

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Glucose had no effect on initial nitrate reductase induction in corn root tips and mature sections.

Taxon
Zea mays L.Zea mayse0
Treatment
addition of glucosee0
Outcome
induction of nitrate reductasee0
Direction
no effecte0
Qualifier
during the initial 3 hourse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In Zea mays L., addition of glucose to the induction medium has no effect on the induction of nitrate reductase during the initial 3 hours either in root tips (0-10 mm) or mature root sections (25-35 mm).”

✓ located verbatim in the source · abstract, characters 0–204 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At longer times, glucose increased nitrate reductase activity in both corn root segments.

Taxon
Zea mays L.Zea mayse1
Treatment
glucosee0
Outcome
enzyme activitye0
Direction
highere0
Qualifier
With longer timese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“With longer times, higher levels of enzyme activity are recovered from both root segments when glucose is present in the incubation medium.”

✓ located verbatim in the source · abstract, characters 205–344 · show in text ↑
e1

“Zea mays L.”

✓ located verbatim in the source · abstract, characters 3–14 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Nitrate reductase induction in corn root tips saturated at 10 mm nitrate.

Taxon
Zea mays L.Zea mayse1
Organ / stage
root tipse0
Outcome
induction in root tipse0
Direction
saturatede0
Value
10 mm NO(3) (-)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The induction in root tips is saturated by 10 mm NO(3) (-).”

✓ located verbatim in the source · abstract, characters 345–404 · show in text ↑
e1

“Zea mays L.”

✓ located verbatim in the source · abstract, characters 3–14 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Mature corn root sections required higher nitrate concentrations for saturation.

Taxon
Zea mays L.Zea mayse1
Organ / stage
mature root sectionse0
Outcome
saturatione0
Direction
Highere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Higher concentrations of NO(3) (-) are required for saturation in mature root sections.”

✓ located verbatim in the source · abstract, characters 405–492 · show in text ↑
e1

“Zea mays L.”

✓ located verbatim in the source · abstract, characters 3–14 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Corn root glucose response occurred across a wide range of external nitrate concentrations.

Taxon
Zea mays L.Zea mayse1
Outcome
response to glucosee0
Qualifier
over a wide range of external NO(3) (-) concentrationse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The response to glucose is seen over a wide range of external NO(3) (-) concentrations.”

✓ located verbatim in the source · abstract, characters 493–580 · show in text ↑
e1

“Zea mays L.”

✓ located verbatim in the source · abstract, characters 3–14 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Nitrate reductase activity was rapidly lost after nitrate withdrawal.

Taxon
Zea mays L.Zea mayse1
Treatment
nitrate is withdrawne0
Outcome
Nitrate reductase activitye0
Direction
loste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Nitrate reductase activity is lost rapidly when nitrate is withdrawn from the induction medium.”

✓ located verbatim in the source · abstract, characters 580–675 · show in text ↑
e1

“Zea mays L.”

✓ located verbatim in the source · abstract, characters 3–14 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Glucose did not prevent nitrate reductase activity loss after nitrate withdrawal.

Taxon
Zea mays L.Zea mayse1
Treatment
Additions of glucosee0
Outcome
this losse0
Direction
do not prevente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Nitrate reductase activity is lost rapidly when nitrate is withdrawn from the induction medium. Additions of glucose do not prevent this loss.”

✓ located verbatim in the source · abstract, characters 580–722 · show in text ↑
e1

“Zea mays L.”

✓ located verbatim in the source · abstract, characters 3–14 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Glucose had no effect on total nitrate uptake by corn root segments.

Taxon
Zea mays L.Zea mayse1
Treatment
Additions of glucosee0
Outcome
total uptake of NO(3) (-)e0
Direction
no effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Additions of glucose have no effect on total uptake of NO(3) (-) by the root segments”

✓ located verbatim in the source · abstract, characters 723–808 · show in text ↑
e1

“Zea mays L.”

✓ located verbatim in the source · abstract, characters 3–14 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Glucose increased anaerobic nitrite production in corn root tips and mature segments.

Taxon
Zea mays L.Zea mayse2
Treatment
Additions of glucosee1
Outcome
anaerobic NO(2) (-) productione0
Direction
increasee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“they increase the anaerobic NO(2) (-) production in both root tips and mature root segments”

✓ located verbatim in the source · abstract, characters 813–904 · show in text ↑
e1

“Additions of glucose have no effect on total uptake”

✓ located verbatim in the source · abstract, characters 723–774 · show in text ↑
e2

“Zea mays L.”

✓ located verbatim in the source · abstract, characters 3–14 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors concluded that glucose alters nitrate distribution within corn root sections.

Taxon
Zea mays L.Zea mayse1
Treatment
glucosee0
Outcome
distribution of NO(3) (-) within the root sectionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Thus the results show that glucose alters the distribution of NO(3) (-) within the root sections.”

✓ located verbatim in the source · abstract, characters 1008–1105 · show in text ↑
e1

“Zea mays L.”

✓ located verbatim in the source · abstract, characters 3–14 · show in text ↑

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