Abstract
ABA-INSENSITIVE 3 (ABI3) has long been known for activation of storage protein accumulation. A role of ABI3 on oil accumulation was previously suggested based on a decrease of oil content in seeds of abi3 mutant. However, this conclusion could not exclude possibilities of indirect or pleiotropic effects, such as through mutual regulatory interactions with FUSCA3 (FUS3), an activator of oil accumulation. To identify that ABI3 functions independent of the effects of related seed transcription factors, we expressed ABI3 under the control of an inducible promoter in tobacco BY2 cells and Arabidopsis rosette leaves. Inducible expression of ABI3 activated oil accumulation in these non-seed cells, demonstrating a general role of ABI3 in regulation of oil biosynthesis. Further expressing ABI3 in rosette leaves of fus3 knockout mutant still caused up to 3-fold greater triacylglycerol accumulation, indicating ABI3 can activate lipid accumulation independently of FUS3. Transcriptome analysis revealed that LIPID DROPLET PROTEIN (LDP) genes, including OLEOSINs and CALEOSINs, were up-regulated up to 1000-fold by ABI3 in the absence of FUS3, while the expression of WRINKLED1 was doubled. Taken together, our results provide genetic evidence that ABI3 activates oil accumulation with or without FUS3, most likely through up-regulating LDPs and WRINKLED1.
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Claims 5
Claim · model-written summary
Induced ABI3 expression activated oil accumulation in non-seed tobacco and Arabidopsis cells.
- Taxon
- tobacco
Nicotiana tabacume1 - Organ / stage
- non-seed cellse0
- Treatment
- Inducible expression of ABI3e0
- Gene
- ABI3e0
- Outcome
- oil accumulatione0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inducible expression of ABI3 activated oil accumulation in these non-seed cells”
e1“tobacco BY2 cells and Arabidopsis rosette leaves”
Claim · model-written summary
ABI3 expression increased triacylglycerol accumulation up to threefold in fus3 knockout rosette leaves.
- Taxon
- Arabidopsis
Arabidopsise1 - Material
- fus3 knockout mutante0
- Organ / stage
- rosette leavese0
- Treatment
- expressing ABI3e0
- Gene
- ABI3e0
- Outcome
- triacylglycerol accumulatione0
- Direction
- greatere0
- Value
- up to 3-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expressing ABI3 in rosette leaves of fus3 knockout mutant still caused up to 3-fold greater triacylglycerol accumulation”
e1“tobacco BY2 cells and Arabidopsis rosette leaves”
Claim · model-written summary
ABI3 up-regulated LDP genes including OLEOSINs and CALEOSINs up to 1000-fold without FUS3.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- ABI3e0
- Gene
- LIPID DROPLET PROTEIN (LDP) genese0
- Outcome
- LIPID DROPLET PROTEIN (LDP) genes, including OLEOSINs and CALEOSINse0
- Direction
- up-regulatede0
- Value
- up to 1000-folde0
- Qualifier
- in the absence of FUS3e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LIPID DROPLET PROTEIN (LDP) genes, including OLEOSINs and CALEOSINs, were up-regulated up to 1000-fold by ABI3 in the absence of FUS3”
e1“tobacco BY2 cells and Arabidopsis rosette leaves”
Claim · model-written summary
ABI3 doubled WRINKLED1 expression in the absence of FUS3.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- ABI3e2
- Gene
- WRINKLED1e0
- Outcome
- expression of WRINKLED1e0
- Direction
- doublede0
- Value
- doublede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while the expression of WRINKLED1 was doubled”
e1“tobacco BY2 cells and Arabidopsis rosette leaves”
e2“up-regulated up to 1000-fold by ABI3 in the absence of FUS3”
Claim · model-written summary
The authors infer that ABI3 activates oil accumulation independently of FUS3, likely via LDPs and WRINKLED1 up-regulation.
- Taxon
- ArabidopsisArabidopsise1
- Gene
- ABI3e0
- Outcome
- oil accumulatione0
- Qualifier
- most likely through up-regulating LDPs and WRINKLED1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABI3 activates oil accumulation with or without FUS3, most likely through up-regulating LDPs and WRINKLED1”
e1“tobacco BY2 cells and Arabidopsis rosette leaves”
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