Abstract
Nicotine is a major alkaloid accumulating in the vacuole of tobacco (Nicotiana tabacum), but the transporters involved in the vacuolar sequestration are not known. We here report that tobacco genes (NtMATE1 and NtMATE2) encoding transporters of the multidrug and toxic compound extrusion (MATE) family are coordinately regulated with structural genes for nicotine biosynthesis in the root, with respect to spatial expression patterns, regulation by NIC regulatory loci, and induction by methyl jasmonate. Subcellular fractionation, immunogold electron microscopy, and expression of a green fluorescent protein fusion protein all suggested that these transporters are localized to the vacuolar membrane. Reduced expression of the transporters rendered tobacco plants more sensitive to the application of nicotine. In contrast, overexpression of NtMATE1 in cultured tobacco cells induced strong acidification of the cytoplasm after jasmonate elicitation or after the addition of nicotine under nonelicited conditions. Expression of NtMATE1 in yeast (Saccharomyces cerevisiae) cells compromised the accumulation of exogenously supplied nicotine into the yeast cells. The results imply that these MATE-type proteins transport tobacco alkaloids from the cytosol into the vacuole in exchange for protons in alkaloid-synthesizing root cells.
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Claims 6
Claim · model-written summary
NtMATE1 and NtMATE2 were coordinately regulated with nicotine biosynthesis genes across root expression, NIC regulation, and methyl jasmonate induction.
- Taxon
- tobacco
Nicotiana tabacumt0 - Organ / stage
- roote0
- Treatment
- methyl jasmonatee0
- Gene
- NtMATE1 and NtMATE2e0
- Outcome
- coordinately regulated with structural genes for nicotine biosynthesise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco genes (NtMATE1 and NtMATE2) encoding transporters of the multidrug and toxic compound extrusion (MATE) family are coordinately regulated with structural genes for nicotine biosynthesis in the root, with respect to spatial expression patterns, regulation by NIC regulatory loci, and induction by methyl jasmonate”
t0“Tobacco Roots”
Claim · model-written summary
The transporters localized to the vacuolar membrane.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- localized to the vacuolar membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these transporters are localized to the vacuolar membrane”
t0“Tobacco Roots”
Claim · model-written summary
Reduced transporter expression increased tobacco sensitivity to applied nicotine.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Reduced expression of the transporterse0
- Outcome
- sensitive to the application of nicotinee0
- Direction
- more sensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reduced expression of the transporters rendered tobacco plants more sensitive to the application of nicotine”
Claim · model-written summary
NtMATE1 overexpression caused strong cytoplasmic acidification after jasmonate elicitation or nicotine addition.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- overexpression of NtMATE1e0
- Gene
- NtMATE1e0
- Outcome
- acidification of the cytoplasme0
- Direction
- inducede0
- Qualifier
- after jasmonate elicitation or after the addition of nicotine under nonelicited conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of NtMATE1 in cultured tobacco cells induced strong acidification of the cytoplasm after jasmonate elicitation or after the addition of nicotine under nonelicited conditions”
Claim · model-written summary
NtMATE1 expression compromised exogenous nicotine accumulation in yeast cells.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- Expression of NtMATE1 in yeaste0
- Gene
- NtMATE1e0
- Outcome
- accumulation of exogenously supplied nicotine into the yeast cellse0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of NtMATE1 in yeast (Saccharomyces cerevisiae) cells compromised the accumulation of exogenously supplied nicotine into the yeast cells”
t0“Tobacco Roots”
Claim · model-written summary
The authors infer that MATE proteins exchange protons for alkaloids to transport them into root vacuoles.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- root cellse0
- Gene
- MATE-type proteinse0
- Outcome
- transport tobacco alkaloids from the cytosol into the vacuolee0
- Qualifier
- in exchange for protonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these MATE-type proteins transport tobacco alkaloids from the cytosol into the vacuole in exchange for protons in alkaloid-synthesizing root cells”
The same questions in other papers
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