Abstract
The mechanisms controlling seed dormancy in Arabidopsis (Arabidopsis thaliana) have been characterized by proteomics using the dormant (D) accession Cvi originating from the Cape Verde Islands. Comparative studies carried out with freshly harvested dormant and after-ripened non-dormant (ND) seeds revealed a specific differential accumulation of 32 proteins. The data suggested that proteins associated with metabolic functions potentially involved in germination can accumulate during after-ripening in the dry state leading to dormancy release. Exogenous application of abscisic acid (ABA) to ND seeds strongly impeded their germination, which physiologically mimicked the behavior of D imbibed seeds. This application resulted in an alteration of the accumulation pattern of 71 proteins. There was a strong down-accumulation of a major part (90%) of these proteins, which were involved mainly in energetic and protein metabolisms. This feature suggested that exogenous ABA triggers proteolytic mechanisms in imbibed seeds. An analysis of de novo protein synthesis by two-dimensional gel electrophoresis in the presence of [(35)S]-methionine disclosed that exogenous ABA does not impede protein biosynthesis during imbibition. Furthermore, imbibed D seeds proved competent for de novo protein synthesis, demonstrating that impediment of protein translation was not the cause of the observed block of seed germination. However, the two-dimensional protein profiles were markedly different from those obtained with the ND seeds imbibed in ABA. Altogether, the data showed that the mechanisms blocking germination of the ND seeds by ABA application are different from those preventing germination of the D seeds imbibed in basal medium.
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Claims 9
Claim · model-written summary
Dormant and after-ripened non-dormant seeds differed in accumulation of 32 proteins.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- freshly harvested dormant and after-ripened non-dormant (ND) seedse1
- Outcome
- differential accumulatione1
- Value
- 32 proteinse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed dormancy in Arabidopsis (Arabidopsis thaliana)”
e1“Comparative studies carried out with freshly harvested dormant and after-ripened non-dormant (ND) seeds revealed a specific differential accumulation of 32 proteins”
Claim · model-written summary
Exogenous ABA applied to non-dormant seeds strongly impeded germination.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- ND seedse2
- Treatment
- Exogenous application of abscisic acid (ABA)e2
- Outcome
- germinatione2
- Direction
- impedede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed dormancy in Arabidopsis (Arabidopsis thaliana)”
e2“Exogenous application of abscisic acid (ABA) to ND seeds strongly impeded their germination”
Claim · model-written summary
Exogenous ABA altered the accumulation pattern of 71 proteins.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Exogenous application of abscisic acid (ABA)e2
- Outcome
- accumulation patterne3
- Value
- 71 proteinse3
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed dormancy in Arabidopsis (Arabidopsis thaliana)”
e2“Exogenous application of abscisic acid (ABA) to ND seeds strongly impeded their germination”
e3“This application resulted in an alteration of the accumulation pattern of 71 proteins”
Claim · model-written summary
A major part (90%) of the ABA-affected proteins were strongly down-accumulated.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Exogenous application of abscisic acid (ABA)e2
- Outcome
- down-accumulation of a major parte4
- Direction
- down-accumulatione4
- Value
- 90%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed dormancy in Arabidopsis (Arabidopsis thaliana)”
e2“Exogenous application of abscisic acid (ABA) to ND seeds strongly impeded their germination”
e4“There was a strong down-accumulation of a major part (90%) of these proteins”
Claim · model-written summary
Exogenous ABA does not impede protein biosynthesis during imbibition.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- exogenous ABAe5
- Outcome
- protein biosynthesis during imbibitione5
- Direction
- does not impedee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed dormancy in Arabidopsis (Arabidopsis thaliana)”
e5“disclosed that exogenous ABA does not impede protein biosynthesis during imbibition”
Claim · model-written summary
Imbibed D seeds were competent for de novo protein synthesis.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- imbibed D seedse6
- Outcome
- de novo protein synthesise6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed dormancy in Arabidopsis (Arabidopsis thaliana)”
e6“imbibed D seeds proved competent for de novo protein synthesis”
Claim · model-written summary
Two-dimensional protein profiles of imbibed D seeds were markedly different from those of ND seeds imbibed in ABA.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- two-dimensional protein profilese7
- Qualifier
- markedly differente7
- Comparator
- ND seeds imbibed in ABAe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed dormancy in Arabidopsis (Arabidopsis thaliana)”
e7“the two-dimensional protein profiles were markedly different from those obtained with the ND seeds imbibed in ABA”
Claim · model-written summary
The mechanisms blocking germination of ND seeds by ABA differ from those blocking germination of D seeds imbibed in basal medium.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- germinatione1
- Qualifier
- the mechanisms blocking germination of the ND seeds by ABA application are different from those preventing germination of the D seeds imbibed in basal mediume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed dormancy in Arabidopsis (Arabidopsis thaliana)”
e1“the mechanisms blocking germination of the ND seeds by ABA application are different from those preventing germination of the D seeds imbibed in basal medium”
Claim · model-written summary
Proteins of metabolic functions involved in germination may accumulate during after-ripening and lead to dormancy release (hypothesis).
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- dormancy releasee1
- Qualifier
- proteins associated with metabolic functions potentially involved in germination can accumulate during after-ripening in the dry statee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed dormancy in Arabidopsis (Arabidopsis thaliana)”
e1“proteins associated with metabolic functions potentially involved in germination can accumulate during after-ripening in the dry state leading to dormancy release”
The same questions in other papers
(+)-abscisic acid · quality: protein · ArabidopsisPositive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.