Abstract
Many cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA) as well as by cold. This has been thought to occur via two separate signaling pathways, with ABA acting via ABA-responsive promoter elements and low temperature activating the C-repeat element (CRT; dehydration-responsive) promoter element via CBF (DREB1) transcription factors. We show here that ABA is also capable of activating the CRT promoter element. Although the more recently discovered ABA-inducible CBF4 transcription factor might have accounted for this, we show here that CBF1-3 transcript levels also increase in response to elevated ABA levels. This increase in CBF1-3 transcript levels appears to be at least in part due to increased activity of the CBF promoters in response to ABA. A total of 125 bp of the CBF2 promoter, which has previously been shown to be sufficient for cold-, mechanical-, and cycloheximide-induced expression, was also sufficient for ABA-induced expression. However, the ABA-responsive promoter element-like motif within this region is not needed for ABA-induced expression. An observed increase in CBF protein levels after ABA treatment, together with previous data showing that increased CBF levels are sufficient for cold-regulated gene induction, suggests that ABA-induced increases in CBF1-3 transcript levels do have the potential to activate the CRT. Our data indicate therefore that activation of the CRT may also occur via a novel ABA-inducible signaling pathway using the normally cold-inducible CBFs.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
ABA is capable of activating the CRT promoter element in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- ABAe1
- Outcome
- activating the CRT promoter elemente1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA)”
e1“We show here that ABA is also capable of activating the CRT promoter element”
Claim · model-written summary
CBF1-3 transcript levels increase in Arabidopsis in response to elevated ABA levels.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- elevated ABA levelse2
- Gene
- CBF1-3e2
- Outcome
- CBF1-3 transcript levelse2
- Direction
- increasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA)”
e2“CBF1-3 transcript levels also increase in response to elevated ABA levels”
Claim · model-written summary
The ABA-induced increase in CBF1-3 transcripts appears to be due at least in part to increased activity of the CBF promoters.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- ABAe3
- Gene
- CBF promoterse3
- Outcome
- CBF1-3 transcript levelse3
- Qualifier
- increased activity of the CBF promoterse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA)”
e3“This increase in CBF1-3 transcript levels appears to be at least in part due to increased activity of the CBF promoters in response to ABA”
Claim · model-written summary
A 125 bp segment of the CBF2 promoter was sufficient for ABA-induced expression.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- ABAe4
- Gene
- CBF2 promotere4
- Outcome
- ABA-induced expressione4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA)”
e4“A total of 125 bp of the CBF2 promoter, which has previously been shown to be sufficient for cold-, mechanical-, and cycloheximide-induced expression, was also sufficient for ABA-induced expression”
Claim · model-written summary
The ABA-responsive promoter element-like motif in the CBF2 promoter region is not needed for ABA-induced expression.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- ABAe5
- Outcome
- ABA-induced expressione5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA)”
e5“the ABA-responsive promoter element-like motif within this region is not needed for ABA-induced expression”
Claim · model-written summary
CBF protein levels increase in Arabidopsis after ABA treatment.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- ABA treatmente6
- Outcome
- CBF protein levelse6
- Direction
- increasee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA)”
e6“An observed increase in CBF protein levels after ABA treatment”
Claim · model-written summary
The data suggest that ABA may activate the CRT through a novel ABA-inducible pathway using the cold-inducible CBFs.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- CBFse7
- Outcome
- activation of the CRTe7
- Qualifier
- novel ABA-inducible signaling pathwaye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Many cold-regulated genes of Arabidopsis are inducible by abscisic acid (ABA)”
e7“activation of the CRT may also occur via a novel ABA-inducible signaling pathway using the normally cold-inducible CBFs”
The same questions in other papers
(+)-abscisic acid · molecular: expression · ArabidopsisPositive and negative results
24 papers in the same direction as this paper · 57 in the opposite direction
- positive
- positive
- positive
78 papers more in this group
See all 82 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.