Abstract
Triacylglycerol (TAG) is the main storage lipid in plant seeds and the major form of plant oil used for food and, increasingly, for industrial and biofuel applications. Several transcription factors, including FUSCA3 (At3 g26790, FUS3), are associated with embryo maturation and oil biosynthesis in seeds. However, the ability of FUS3 to increase TAG biosynthesis in other tissues has not been quantitatively examined. Here, we evaluated the ability of FUS3 to activate TAG accumulation in non-seed tissues. Overexpression of FUS3 driven by an estradiol-inducible promoter increased oil contents in Arabidopsis seedlings up to 6% of dry weight; more than 50-fold over controls. Eicosenoic acid, a characteristic fatty acid of Arabidopsis seed oil, accumulated to over 20% of fatty acids in cotyledons and leaves. These large increases depended on added sucrose, although without sucrose TAG increased three- to four-fold. Inducing the expression of FUS3 in tobacco BY2 cells also increased TAG accumulation, and co-expression of FUS3 and diacylglycerol acyltransferase 1 (DGAT1) further increased TAG levels to 4% of dry weight. BY2 cell growth was not altered by FUS3 expression, although Arabidopsis seedling development was impaired, consistent with the ability of FUS3 to induce embryo characteristics in non-seed tissues. Microarrays of Arabidopsis seedlings revealed that FUS3 overexpression increased the expression of a higher proportion of genes involved in TAG biosynthesis than genes involved in fatty acid biosynthesis or other lipid pathways. Together these results provide additional insights into FUS3 functions in TAG metabolism and suggest complementary strategies for engineering vegetative oil accumulation.
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Claims 8
Claim · model-written summary
FUS3 overexpression increased Arabidopsis seedling oil to 6% dry weight, over 50-fold controls.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- seedlingse0
- Treatment
- Overexpression of FUS3e0
- Gene
- FUS3e0
- Outcome
- oil contentse0
- Direction
- increasede0
- Value
- 6% of dry weighte0
- Comparator
- controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of FUS3 driven by an estradiol-inducible promoter increased oil contents in Arabidopsis seedlings up to 6% of dry weight; more than 50-fold over controls”
Claim · model-written summary
Eicosenoic acid reached over 20% of fatty acids in Arabidopsis cotyledons and leaves.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- cotyledons and leavese0
- Outcome
- Eicosenoic acide0
- Direction
- accumulatede0
- Value
- over 20% of fatty acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Eicosenoic acid, a characteristic fatty acid of Arabidopsis seed oil, accumulated to over 20% of fatty acids in cotyledons and leaves”
Claim · model-written summary
Without sucrose FUS3 increased TAG three- to four-fold.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- without sucrosee0
- Outcome
- TAGe0
- Direction
- increasede0
- Value
- three- to four-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“without sucrose TAG increased three- to four-fold”
t0“FUSCA3 activates triacylglycerol accumulation in Arabidopsis seedlings”
Claim · model-written summary
FUS3 induction increased TAG accumulation in tobacco BY2 cells.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- BY2 cellse0
- Treatment
- Inducing the expression of FUS3e0
- Gene
- FUS3e0
- Outcome
- TAG accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inducing the expression of FUS3 in tobacco BY2 cells also increased TAG accumulation”
Claim · model-written summary
FUS3 and DGAT1 co-expression further increased TAG to 4% dry weight in tobacco BY2 cells.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- co-expression of FUS3 and diacylglycerol acyltransferase 1 (DGAT1)e0
- Gene
- DGAT1e0
- Outcome
- TAG levelse0
- Direction
- increasede0
- Value
- 4% of dry weighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“co-expression of FUS3 and diacylglycerol acyltransferase 1 (DGAT1) further increased TAG levels to 4% of dry weight”
e1“tobacco BY2 cells”
Claim · model-written summary
FUS3 expression did not alter BY2 cell growth.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- FUS3 expressione0
- Gene
- FUS3e0
- Outcome
- BY2 cell growthe0
- Direction
- not alterede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BY2 cell growth was not altered by FUS3 expression”
e1“tobacco BY2 cells”
Claim · model-written summary
FUS3 expression impaired Arabidopsis seedling development.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- FUS3 expressione1
- Outcome
- seedling developmente0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis seedling development was impaired”
e1“FUS3 expression”
Claim · model-written summary
FUS3 overexpression increased expression of a higher proportion of TAG biosynthesis genes than fatty acid biosynthesis or other lipid pathway genes.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- FUS3 overexpressione0
- Gene
- FUS3e0
- Outcome
- expression of a higher proportion of genes involved in TAG biosynthesise0
- Direction
- increasede0
- Comparator
- genes involved in fatty acid biosynthesis or other lipid pathwayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FUS3 overexpression increased the expression of a higher proportion of genes involved in TAG biosynthesis than genes involved in fatty acid biosynthesis or other lipid pathways”
e1“Microarrays of Arabidopsis seedlings”
The same questions in other papers
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- no effect
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See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.