Abstract
In transgenic tobacco (Nicotiana tabacum L.) seed, expression of chimeric [beta]-glucuronidase (GUS) genes containing the vicilin or napin storage-protein gene promoters is responsive to premature drying and declines upon rehydration (L. Jiang, W.L. Downing, C.L. Baszczynski, A.R. Kermode [1995] Plant Physiol 107: 1439-1449). Desiccation may cause changes in the content of or sensitivity to abscisic acid (ABA), partially or wholly removing the effects of this "modulator" of developmental gene expression. We studied the enhancement of GUS reporter enzyme activities in transgenic tobacco by exogenous ABA before and after drying. A racemic mixture of ABA at 10 [mu]M caused a 2- to 3-fold increase in GUS activity in developing transgenic seed expressing chimeric genes containing the vicilin or napin gene promoters. However, when these seeds were prematurely dried prior to their culture on ABA medium, enhancement of GUS activity was virtually abolished. Use of optically pure ABAs revealed that the enhancement in GUS activity in developing fresh seed was due largely to the natural (+) form of ABA. Chimeric constructs containing a viral 35S promoter did not respond to ABA whether or not premature drying was applied. Thus, vicilin and napin chimeric genes, initially sensitive to ABA, become relatively insensitive to the hormone following drying. A decline in ABA sensitivity may be an important factor in the cessation of storage-protein gene expression.
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Claims 6
Claim · model-written summary
ABA at 10 µM increased GUS activity 2- to 3-fold in developing transgenic tobacco seed carrying vicilin or napin promoter constructs.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Organ / stage
- developing transgenic seede1
- Treatment
- ABA at 10 [mu]Me1
- Gene
- vicilin or napin gene promoterse1
- Outcome
- GUS activitye1
- Direction
- increasee1
- Value
- 2- to 3-folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco (Nicotiana tabacum L.) seed”
e1“A racemic mixture of ABA at 10 [mu]M caused a 2- to 3-fold increase in GUS activity in developing transgenic seed expressing chimeric genes containing the vicilin or napin gene promoters”
Claim · model-written summary
Premature drying of seed virtually abolished the enhancement of GUS activity by ABA, relative to developing fresh seed.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- prematurely dried prior to their culture on ABA mediume1
- Outcome
- enhancement of GUS activitye1
- Direction
- virtually abolishede1
- Comparator
- developing fresh seede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco (Nicotiana tabacum L.) seed”
e1“However, when these seeds were prematurely dried prior to their culture on ABA medium, enhancement of GUS activity was virtually abolished”
e2“Use of optically pure ABAs revealed that the enhancement in GUS activity in developing fresh seed was due largely to the natural (+) form of ABA”
Claim · model-written summary
The enhancement of GUS activity in developing fresh seed is attributed to the natural (+) form of ABA.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Organ / stage
- developing fresh seede2
- Compound
- natural (+) form of ABAe2
- Outcome
- enhancement in GUS activitye2
- Qualifier
- due largely toe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco (Nicotiana tabacum L.) seed”
e2“Use of optically pure ABAs revealed that the enhancement in GUS activity in developing fresh seed was due largely to the natural (+) form of ABA”
Claim · model-written summary
Constructs with a viral 35S promoter did not respond to ABA, whether or not seed was prematurely dried.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- ABAe3
- Gene
- viral 35S promotere3
- Outcome
- respond to ABAe3
- Direction
- did not responde3
- Qualifier
- whether or not premature drying was appliede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco (Nicotiana tabacum L.) seed”
e3“Chimeric constructs containing a viral 35S promoter did not respond to ABA whether or not premature drying was applied”
Claim · model-written summary
Vicilin and napin chimeric genes, initially sensitive to ABA, became relatively insensitive to ABA after drying.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- dryinge4
- Gene
- vicilin and napin chimeric genese4
- Outcome
- sensitive to ABAe4
- Direction
- become relatively insensitivee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco (Nicotiana tabacum L.) seed”
e4“Thus, vicilin and napin chimeric genes, initially sensitive to ABA, become relatively insensitive to the hormone following drying”
Claim · model-written summary
A decline in ABA sensitivity may be an important factor in the cessation of storage-protein gene expression.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Outcome
- cessation of storage-protein gene expressione5
- Qualifier
- A decline in ABA sensitivitye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In transgenic tobacco (Nicotiana tabacum L.) seed”
e5“A decline in ABA sensitivity may be an important factor in the cessation of storage-protein gene expression”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
5 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.