Abstract
The tomato accession LA1996, which carries a dominant allele of anthocyanin fruit (Aft) locus, accumulates anthocyanins in the epidermis of fruits when exposed to sunlight. The involvement of blue, UV-A, UV-B and a combination of these wavelengths on anthocyanin accumulation and the molecular mechanism of their regulation was investigated in LA1996. The most effective treatment for inducing anthocyanin biosynthesis in Aft fruits was co-irradiation with blue and UV-B (blue + UV-B) light. Finding the correlated genes is an important approach towards understanding their molecular mechanisms. In the present study, the nitrate reductase (NR) gene SlNIA was isolated using RNA-seq profiling of Aft fruits given different light treatments. The functions of NR-mediated anthocyanin induction by blue + UV-B were confirmed using a series of chemical treatments, followed by assessment of NR activity and nitric oxide (NO) detection. The expression of NR was highly induced by blue + UV-B, and this specificity was also confirmed with the enzyme activity of NR and the NO concentration. The NR inhibitors, which reduce NO generation, the expression levels of anthocyanin related genes and decreased anthocyanin accumulation in LA1996. Our results suggest that NR plays a key role in blue + UV-B-mediated anthocyanin accumulation in LA1996 fruits.
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Claims 7
Claim · model-written summary
Blue plus UV-B was the most effective light treatment for anthocyanin biosynthesis in Aft fruit.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- Afte0
- Treatment
- co-irradiation with blue and UV-B (blue + UV-B) lighte0
- Outcome
- anthocyanin biosynthesise0
- Direction
- most effectivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“The most effective treatment for inducing anthocyanin biosynthesis in Aft fruits was co-irradiation with blue and UV-B (blue + UV-B) light.”
Claim · model-written summary
RNA-seq identified SlNIA in Aft fruits under different light treatments.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- Afte0
- Treatment
- different light treatmentse0
- Gene
- SlNIAe0
- Outcome
- SlNIA was isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“the nitrate reductase (NR) gene SlNIA was isolated using RNA-seq profiling of Aft fruits given different light treatments.”
Claim · model-written summary
Blue plus UV-B highly induced nitrate reductase expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- blue + UV-Be0
- Gene
- NRe0
- Outcome
- expression of NRe0
- Direction
- highly inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“The expression of NR was highly induced by blue + UV-B”
Claim · model-written summary
Nitrate reductase activity and nitric oxide concentration showed the same blue plus UV-B specificity.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- NRe0
- Outcome
- enzyme activity of NR and the NO concentratione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“this specificity was also confirmed with the enzyme activity of NR and the NO concentration.”
Claim · model-written summary
NR inhibitors reduced NO generation and anthocyanin related gene expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- LA1996e0
- Treatment
- NR inhibitorse0
- Outcome
- NO generation, the expression levels of anthocyanin related genese0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“The NR inhibitors, which reduce NO generation, the expression levels of anthocyanin related genes and decreased anthocyanin accumulation in LA1996.”
Claim · model-written summary
NR inhibitors decreased anthocyanin accumulation in LA1996.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- LA1996e0
- Treatment
- NR inhibitorse0
- Outcome
- anthocyanin accumulatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“The NR inhibitors, which reduce NO generation, the expression levels of anthocyanin related genes and decreased anthocyanin accumulation in LA1996.”
Claim · model-written summary
NR was proposed to play a key role in blue plus UV-B-mediated anthocyanin accumulation.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- LA1996e0
- Gene
- NRe0
- Outcome
- anthocyanin accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“tomato”
e0“Our results suggest that NR plays a key role in blue + UV-B-mediated anthocyanin accumulation in LA1996 fruits.”
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See all 27 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.