Abstract
The footprint of tomato cultivation, a cool region crop that exhibits heat stress (HS) sensitivity, is increasing in the tropics/sub-tropics. Knowledge of novel regulatory hot spots from varieties growing in the Indian sub-continent climatic zones could be vital for developing HS-resilient crops. Comparative transcriptome-wide signatures of a tolerant (CLN1621L) and sensitive (CA4) cultivar pair shortlisted from a pool of varieties exhibiting variable thermo-sensitivity using physiological-, survival- and yield-related traits revealed redundant to cultivar-specific HS regulation. The antagonistically expressing genes encode enzymes and proteins that have roles in plant defence and abiotic stresses. Functional characterization of three antagonistic genes by overexpression and silencing established Solyc09g014280 (Acylsugar acyltransferase) and Solyc07g056570 (Notabilis) that are up-regulated in tolerant cultivar, as positive regulators of HS tolerance and Solyc03g020030 (Pin-II proteinase inhibitor), that are down-regulated in CLN1621L, as negative regulator of thermotolerance. Transcriptional assessment of promoters of these genes by SNPs in stress-responsive cis-elements and promoter swapping experiments in opposite cultivar background showed inherent cultivar-specific orchestration of transcription factors in regulating transcription. Moreover, overexpression of three ethylene response transcription factors (ERF.C1/F4/F5) also improved HS tolerance in tomato. This study identifies several novel HS tolerance genes and provides proof of their utility in tomato thermotolerance.
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Claims 7
Claim · model-written summary
Tolerant and sensitive cultivars showed shared and cultivar-specific heat stress regulation.
- Taxon
- tomato
Solanum lycopersicumt0 - Material
- CLN1621Le0
- Outcome
- redundant to cultivar-specific HS regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparative transcriptome-wide signatures of a tolerant (CLN1621L) and sensitive (CA4) cultivar pair shortlisted from a pool of varieties exhibiting variable thermo-sensitivity using physiological-, survival- and yield-related traits revealed redundant to cultivar-specific HS regulation.”
t0“tomato”
Claim · model-written summary
Antagonistically expressed genes encode proteins involved in plant defence and abiotic stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- enzymes and proteins that have roles in plant defence and abiotic stressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The antagonistically expressing genes encode enzymes and proteins that have roles in plant defence and abiotic stresses.”
t0“tomato”
Claim · model-written summary
Solyc09g014280 was established as a positive regulator of heat tolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- overexpression and silencinge0
- Gene
- Solyc09g014280e0
- Outcome
- positive regulators of HS tolerancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional characterization of three antagonistic genes by overexpression and silencing established Solyc09g014280 (Acylsugar acyltransferase) and Solyc07g056570 (Notabilis) that are up-regulated in tolerant cultivar, as positive regulators of HS tolerance”
t0“tomato”
Claim · model-written summary
Solyc07g056570 was established as a positive regulator of heat tolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- overexpression and silencinge0
- Gene
- Solyc07g056570e0
- Outcome
- positive regulators of HS tolerancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional characterization of three antagonistic genes by overexpression and silencing established Solyc09g014280 (Acylsugar acyltransferase) and Solyc07g056570 (Notabilis) that are up-regulated in tolerant cultivar, as positive regulators of HS tolerance”
t0“tomato”
Claim · model-written summary
Solyc03g020030 was established as a negative regulator of thermotolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- overexpression and silencinge1
- Gene
- Solyc03g020030e0
- Outcome
- negative regulator of thermotolerancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Solyc03g020030 (Pin-II proteinase inhibitor), that are down-regulated in CLN1621L, as negative regulator of thermotolerance.”
e1“Functional characterization of three antagonistic genes by overexpression and silencing”
t0“tomato”
Claim · model-written summary
Promoter assessments and swaps showed cultivar-specific transcription factor orchestration.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- inherent cultivar-specific orchestration of transcription factors in regulating transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptional assessment of promoters of these genes by SNPs in stress-responsive cis-elements and promoter swapping experiments in opposite cultivar background showed inherent cultivar-specific orchestration of transcription factors in regulating transcription.”
t0“tomato”
Claim · model-written summary
Overexpression of ERF.C1/F4/F5 improved tomato heat tolerance.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- overexpressione0
- Gene
- ERF.C1/F4/F5e0
- Outcome
- HS tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of three ethylene response transcription factors (ERF.C1/F4/F5) also improved HS tolerance in tomato.”
e1“HS tolerance in tomato”
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