Abstract
Abstract Abscisic acid (ABA) is a phytohormone essential for plants to respond to various environmental stresses, and abscisic acid-insensitive 5 (ABI5) is a basic leucine zipper transcription factor of the ABA signaling pathway. Exogenous ABA induces cold tolerance in bananas; however, the role of MaABI5-like in ABA-induced cold tolerance remains unexplored. The present study found that exogenous ABA alleviated chilling injury of ‘Fenjiao’ banana, induced the accumulation of endogenous ABA, unsaturated fatty acids, and flavonoid content, and reduced the saturated fatty acid content. Moreover, ABA treatment upregulated the transcription levels of MaABI5-like, fatty acid desaturation genes, and flavonoid synthesis-related genes during cold storage. More interestingly, MaABI5-like directly interacted with the promoter of genes related to fatty acid desaturation (MaFAD3-1, MaFAD3-4, MaFAD3-5, MaFAD6-2, MaFAD6-3) and flavonoid synthesis (MaPAL-like, MaPAL-like1, MaC4H-like3, Ma4CL-like1, Ma4CL-like10, MaCHS6-4-like, and MaFLS) and activated their expressions. Furthermore, the transient overexpression of MaABI5-like in ‘Fenjiao’ banana fruit and ectopic expression in tomato plants enhanced cold tolerance and upregulated fatty acid desaturation and flavonoid synthesis-related gene transcript levels. The reduced expression of MaABI5-like by virus-induced gene silencing in ‘Fenjiao’ banana increased chilling injury and downregulated the expression of fatty acid desaturation and flavonoid synthesis-related genes. Thus, the study indicates that MaABI5-like regulates ABA-induced cold tolerance by increasing unsaturated fatty acid and flavonoid content.
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Claims 13
Claim · model-written summary
ABA alleviated chilling injury in Fenjiao banana.
- Taxon
- bananae0
- Material
- ‘Fenjiao’e0
- Treatment
- exogenous ABAe0
- Outcome
- chilling injurye0
- Direction
- alleviatede0
Not reportedComparator
Verbatim
e0“exogenous ABA alleviated chilling injury of ‘Fenjiao’ banana”
Claim · model-written summary
ABA increased endogenous ABA, unsaturated fatty acids and flavonoid content.
- Taxon
- bananat0
- Material
- ‘Fenjiao’t0
- Treatment
- ABAe0
- Outcome
- endogenous ABA, unsaturated fatty acids, and flavonoid contente0
- Direction
- induced the accumulatione0
Not reportedComparator
Verbatim
e0“induced the accumulation of endogenous ABA, unsaturated fatty acids, and flavonoid content”
t0“‘Fenjiao’ banana fruit”
Claim · model-written summary
ABA reduced saturated fatty acid content.
Not reportedComparator
Verbatim
e0“reduced the saturated fatty acid content”
t0“‘Fenjiao’ banana fruit”
e1“ABA treatment upregulated the transcription levels”
Claim · model-written summary
ABA upregulated MaABI5-like transcription during cold storage.
- Taxon
- bananat0
- Treatment
- ABAe0
- Gene
- MaABI5-likee0
- Outcome
- transcription levels of MaABI5-likee0
- Direction
- upregulatede0
- Qualifier
- during cold storagee0
Not reportedComparator
Verbatim
e0“ABA treatment upregulated the transcription levels of MaABI5-like, fatty acid desaturation genes, and flavonoid synthesis-related genes during cold storage”
t0“‘Fenjiao’ banana fruit”
Claim · model-written summary
ABA upregulated fatty acid desaturation genes during cold storage.
- Taxon
- bananat0
- Treatment
- ABAe0
- Gene
- fatty acid desaturation genese0
- Outcome
- transcription levelse0
- Direction
- upregulatede0
Not reportedComparator
Verbatim
e0“ABA treatment upregulated the transcription levels of MaABI5-like, fatty acid desaturation genes, and flavonoid synthesis-related genes during cold storage”
t0“‘Fenjiao’ banana fruit”
Claim · model-written summary
ABA upregulated flavonoid synthesis genes during cold storage.
- Taxon
- bananat0
- Treatment
- ABAe0
- Gene
- flavonoid synthesis-related genese0
- Outcome
- transcription levelse0
- Direction
- upregulatede0
Not reportedComparator
Verbatim
e0“ABA treatment upregulated the transcription levels of MaABI5-like, fatty acid desaturation genes, and flavonoid synthesis-related genes during cold storage”
t0“‘Fenjiao’ banana fruit”
Claim · model-written summary
MaABI5-like bound promoters of fatty acid desaturation genes and activated their expression.
Verbatim
e0“MaABI5-like directly interacted with the promoter of genes related to fatty acid desaturation (MaFAD3-1, MaFAD3-4, MaFAD3-5, MaFAD6-2, MaFAD6-3) and flavonoid synthesis (MaPAL-like, MaPAL-like1, MaC4H-like3, Ma4CL-like1, Ma4CL-like10, MaCHS6-4-like, and MaFLS) and activated their expressions”
t0“‘Fenjiao’ banana fruit”
Claim · model-written summary
MaABI5-like bound promoters of flavonoid synthesis genes and activated their expression.
Verbatim
e0“MaABI5-like directly interacted with the promoter of genes related to fatty acid desaturation (MaFAD3-1, MaFAD3-4, MaFAD3-5, MaFAD6-2, MaFAD6-3) and flavonoid synthesis (MaPAL-like, MaPAL-like1, MaC4H-like3, Ma4CL-like1, Ma4CL-like10, MaCHS6-4-like, and MaFLS) and activated their expressions”
t0“‘Fenjiao’ banana fruit”
Claim · model-written summary
MaABI5-like overexpression enhanced cold tolerance in banana fruit.
- Taxon
- bananae0
- Material
- ‘Fenjiao’e0
- Treatment
- transient overexpression of MaABI5-likee0
- Gene
- MaABI5-likee0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“transient overexpression of MaABI5-like in ‘Fenjiao’ banana fruit and ectopic expression in tomato plants enhanced cold tolerance and upregulated fatty acid desaturation and flavonoid synthesis-related gene transcript levels”
Claim · model-written summary
MaABI5-like ectopic expression enhanced cold tolerance in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- ectopic expressione0
- Gene
- MaABI5-likee0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient overexpression of MaABI5-like in ‘Fenjiao’ banana fruit and ectopic expression in tomato plants enhanced cold tolerance and upregulated fatty acid desaturation and flavonoid synthesis-related gene transcript levels”
Claim · model-written summary
MaABI5-like expression increased fatty acid desaturation and flavonoid synthesis gene transcripts.
- Taxon
- bananae0
- Treatment
- transient overexpression of MaABI5-likee0
- Gene
- MaABI5-likee0
- Outcome
- fatty acid desaturation and flavonoid synthesis-related gene transcript levelse0
- Direction
- upregulatede0
Not reportedComparator
Verbatim
e0“transient overexpression of MaABI5-like in ‘Fenjiao’ banana fruit and ectopic expression in tomato plants enhanced cold tolerance and upregulated fatty acid desaturation and flavonoid synthesis-related gene transcript levels”
Claim · model-written summary
MaABI5-like silencing increased banana chilling injury.
- Taxon
- bananae0
- Material
- ‘Fenjiao’e0
- Treatment
- virus-induced gene silencinge0
- Gene
- MaABI5-likee0
- Outcome
- chilling injurye0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“The reduced expression of MaABI5-like by virus-induced gene silencing in ‘Fenjiao’ banana increased chilling injury and downregulated the expression of fatty acid desaturation and flavonoid synthesis-related genes”
Claim · model-written summary
MaABI5-like silencing downregulated fatty acid desaturation and flavonoid synthesis genes.
- Taxon
- bananae0
- Material
- ‘Fenjiao’e0
- Treatment
- virus-induced gene silencinge0
- Gene
- MaABI5-likee0
- Outcome
- expression of fatty acid desaturation and flavonoid synthesis-related genese0
- Direction
- downregulatede0
Not reportedComparator
Verbatim
e0“The reduced expression of MaABI5-like by virus-induced gene silencing in ‘Fenjiao’ banana increased chilling injury and downregulated the expression of fatty acid desaturation and flavonoid synthesis-related genes”
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overexpression · tolerance: abiotic · Solanum lycopersicum (tomato)Positive and negative results
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See all 6 papers →overexpression · molecular: expression · MusaResults agree
1 paper in the same direction as this paper
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.