Abstract
Because of a high sensitivity to cold, both the yield and quality of tomato (Solanum lycopersicum L.) are severely restricted by cold stress. The NAC transcription factor (TF) family has been characterized as an important player in plant growth, development, and the stress response, but the role of NAC TFs in cold stress and their interaction with other post-transcriptional regulators such as microRNAs in cold tolerance remains elusive. Here, we demonstrated that SlNAM3, the predicted target of Sl-miR164a/b-5p, improved cold tolerance as indicated by a higher maximum quantum efficiency of photosystem II (Fv/Fm), lower relative electrolyte leakage, and less wilting in SlNAM3-overexpression plants compared to wild-type. Further genetic and molecular confirmation revealed that Sl-miR164a/b-5p functioned upstream of SlNAM3 by inhibiting the expression of the latter, thus playing a negative role in cold tolerance. Interestingly, this role is partially mediated by an ethylene-dependent pathway because either Sl-miR164a/b-5p silencing or SlNAM3 overexpression improved cold tolerance in the transgenic lines by promoting ethylene production. Moreover, silencing of the ethylene synthesis genes, SlACS1A, SlACS1B, SlACO1, and SlACO4, resulted in a significant decrease in cold tolerance. Further experiments demonstrated that NAM3 activates SlACS1A, SlACS1B, SlACO1, and SlACO4 transcription by directly binding to their promoters. Taken together, the present study identified the miR164a-NAM3 module conferring cold tolerance in tomato plants via the direct regulation of SlACS1A, SlACS1B, SlACO1, and SlACO4 expression to induce ethylene synthesis.
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Claims 9
Claim · model-written summary
SlNAM3 overexpression improved cold tolerance relative to wild type.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- SlNAM3-overexpressione0
- Gene
- SlNAM3e0
- Outcome
- cold tolerancee0
- Direction
- improvede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlNAM3, the predicted target of Sl-miR164a/b-5p, improved cold tolerance as indicated by a higher maximum quantum efficiency of photosystem II (Fv/Fm), lower relative electrolyte leakage, and less wilting in SlNAM3-overexpression plants compared to wild-type.”
t0“tomato”
Claim · model-written summary
SlNAM3-overexpression plants had higher maximum photosystem II quantum efficiency than wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- SlNAM3-overexpressione0
- Outcome
- maximum quantum efficiency of photosystem II (Fv/Fm)e0
- Direction
- highere0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher maximum quantum efficiency of photosystem II (Fv/Fm), lower relative electrolyte leakage, and less wilting in SlNAM3-overexpression plants compared to wild-type.”
t0“tomato”
Claim · model-written summary
SlNAM3-overexpression plants had lower relative electrolyte leakage than wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- SlNAM3-overexpressione0
- Outcome
- relative electrolyte leakagee0
- Direction
- lowere0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher maximum quantum efficiency of photosystem II (Fv/Fm), lower relative electrolyte leakage, and less wilting in SlNAM3-overexpression plants compared to wild-type.”
t0“tomato”
Claim · model-written summary
SlNAM3-overexpression plants wilted less than wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- SlNAM3-overexpressione0
- Outcome
- wiltinge0
- Direction
- lesse0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher maximum quantum efficiency of photosystem II (Fv/Fm), lower relative electrolyte leakage, and less wilting in SlNAM3-overexpression plants compared to wild-type.”
t0“tomato”
Claim · model-written summary
Sl-miR164a/b-5p inhibited SlNAM3 expression and negatively regulated cold tolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- Sl-miR164a/b-5pe0
- Outcome
- cold tolerancee0
- Qualifier
- inhibiting the expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sl-miR164a/b-5p functioned upstream of SlNAM3 by inhibiting the expression of the latter, thus playing a negative role in cold tolerance.”
t0“tomato”
Claim · model-written summary
Sl-miR164a/b-5p silencing improved cold tolerance by promoting ethylene production.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Sl-miR164a/b-5p silencinge0
- Compound
- ethylenee0
- Outcome
- cold tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“either Sl-miR164a/b-5p silencing or SlNAM3 overexpression improved cold tolerance in the transgenic lines by promoting ethylene production.”
t0“tomato”
Claim · model-written summary
SlNAM3 overexpression improved cold tolerance by promoting ethylene production.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- SlNAM3 overexpressione0
- Compound
- ethylenee0
- Outcome
- cold tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“either Sl-miR164a/b-5p silencing or SlNAM3 overexpression improved cold tolerance in the transgenic lines by promoting ethylene production.”
t0“tomato”
Claim · model-written summary
Silencing ethylene synthesis genes significantly decreased cold tolerance.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- silencing of the ethylene synthesis genese0
- Gene
- SlACS1A, SlACS1B, SlACO1, and SlACO4e0
- Outcome
- cold tolerancee0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of the ethylene synthesis genes, SlACS1A, SlACS1B, SlACO1, and SlACO4, resulted in a significant decrease in cold tolerance.”
t0“tomato”
Claim · model-written summary
NAM3 directly bound promoters and activated transcription of four ethylene synthesis genes.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- NAM3e0
- Outcome
- SlACS1A, SlACS1B, SlACO1, and SlACO4 transcriptione0
- Qualifier
- directly binding to their promoterse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAM3 activates SlACS1A, SlACS1B, SlACO1, and SlACO4 transcription by directly binding to their promoters.”
t0“tomato”
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See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.