Abstract
The impact of simultaneous environmental stresses on plants and how they respond to combined stresses compared with single stresses is largely unclear. By using a transgene (RD29A-LUC) consisting of the firefly luciferase coding sequence (LUC) driven by the stress-responsive RD29A promoter, we investigated the interactive effects of temperature, osmotic stress, and the phytohormone abscisic acid (ABA) in the regulation of gene expression in Arabidopsis seedlings. Results indicated that both positive and negative interactions exist among the studied stress factors in regulating gene expression. At a normal growth temperature (22 degrees C), osmotic stress and ABA act synergistically to induce the transgene expression. Low temperature inhibits the response to osmotic stress or to combined treatment of osmotic stress and ABA, whereas low temperature and ABA treatments are additive in inducing transgene expression. Although high temperature alone does not activate the transgene, it significantly amplifies the effects of ABA and osmotic stress. The effect of multiple stresses in the regulation of RD29A-LUC expression in signal transduction mutants was also studied. The results are discussed in the context of cold and osmotic stress signal transduction pathways.
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Claims 7
Claim · model-written summary
At 22 degrees C, osmotic stress and ABA synergistically induced RD29A-LUC expression.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- osmotic stress and ABAe0
- Gene
- RD29A-LUCe1
- Outcome
- transgene expressione0
- Direction
- inducee0
- Setting
- 22 degrees Ce0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At a normal growth temperature (22 degrees C), osmotic stress and ABA act synergistically to induce the transgene expression.”
t0“Arabidopsis”
e1“By using a transgene (RD29A-LUC)”
Claim · model-written summary
Low temperature inhibited the expression response to osmotic stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Low temperaturee0
- Outcome
- response to osmotic stresse0
- Direction
- inhibitse0
- Comparator
- osmotic stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low temperature inhibits the response to osmotic stress or to combined treatment of osmotic stress and ABA”
t0“Arabidopsis”
Claim · model-written summary
Low temperature inhibited the expression response to combined osmotic stress and ABA.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Low temperaturee0
- Outcome
- responsee0
- Direction
- inhibitse0
- Comparator
- combined treatment of osmotic stress and ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low temperature inhibits the response to osmotic stress or to combined treatment of osmotic stress and ABA”
t0“Arabidopsis”
Claim · model-written summary
Low temperature and ABA had additive effects on transgene expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- low temperature and ABA treatmentse0
- Outcome
- transgene expressione0
- Direction
- inducinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“low temperature and ABA treatments are additive in inducing transgene expression.”
t0“Arabidopsis”
Claim · model-written summary
High temperature alone did not activate the transgene.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- high temperature alonee0
- Outcome
- transgenee0
- Direction
- does not activatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although high temperature alone does not activate the transgene”
t0“Arabidopsis”
Claim · model-written summary
High temperature significantly amplified the effect of ABA on transgene expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- high temperaturee1
- Outcome
- effects of ABAe0
- Direction
- amplifiese0
- Comparator
- ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it significantly amplifies the effects of ABA and osmotic stress.”
t0“Arabidopsis”
e1“Although high temperature alone does not activate the transgene”
Claim · model-written summary
High temperature significantly amplified the effect of osmotic stress on transgene expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- high temperaturee1
- Outcome
- effects of ABA and osmotic stresse0
- Direction
- amplifiese0
- Comparator
- osmotic stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it significantly amplifies the effects of ABA and osmotic stress.”
t0“Arabidopsis”
e1“Although high temperature alone does not activate the transgene”
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