Abstract
To investigate essential components mediating stress signaling in plants, we initiated a large-scale stress response screen using Arabidopsis plants carrying the firefly luciferase reporter gene under the control of the stress-responsive RD29A promoter. Here we report the identification and characterization of a mutant, hos9-1 (for high expression of osmotically responsive genes), in which the reporter construct was hyperactivated by low temperature, but not by abscisic acid or salinity stress. The mutants grow more slowly, and flower later, than do wild-type plants and are more sensitive to freezing, both before and after acclimation, than the wild-type plants. The HOS9 gene encodes a putative homeodomain transcription factor that is localized to the nucleus. HOS9 is constitutively expressed and not further induced by cold stress. Cold treatment increased the level of transcripts of the endogenous RD29A, and some other stress-responsive genes, to a higher level in hos9-1 than in wild-type plants. However, the C repeat/dehydration responsive element-binding factor (CBF) transcription factor genes that mediate a part of cold acclimation in Arabidopsis did not have their response to cold altered by the hos9-1 mutation. Correspondingly, microarray analysis showed that none of the genes affected by the hos9-1 mutation are controlled by the CBF family. Together, these results suggest that HOS9 is important for plant growth and development, and for a part of freezing tolerance, by affecting the activity of genes independent of the CBF pathway.
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Claims 14
Claim · model-written summary
Low temperature hyperactivated the RD29A reporter in hos9-1.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- low temperaturee0
- Outcome
- reporter constructe0
- Direction
- hyperactivatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the reporter construct was hyperactivated by low temperature, but not by abscisic acid or salinity stress”
t0“An Arabidopsis”
Claim · model-written summary
Abscisic acid did not hyperactivate the reporter in hos9-1.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- abscisic acide0
- Outcome
- reporter constructe0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the reporter construct was hyperactivated by low temperature, but not by abscisic acid or salinity stress”
t0“An Arabidopsis”
Claim · model-written summary
Salinity stress did not hyperactivate the reporter in hos9-1.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- salinity stresse0
- Outcome
- reporter constructe0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the reporter construct was hyperactivated by low temperature, but not by abscisic acid or salinity stress”
t0“An Arabidopsis”
Claim · model-written summary
hos9-1 mutants grew more slowly than wild type.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- growe0
- Direction
- more slowlye0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mutants grow more slowly, and flower later, than do wild-type plants”
t0“An Arabidopsis”
Claim · model-written summary
hos9-1 mutants flowered later than wild type.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- flowere0
- Direction
- latere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mutants grow more slowly, and flower later, than do wild-type plants”
t0“An Arabidopsis”
Claim · model-written summary
hos9-1 mutants were more sensitive to freezing before acclimation than wild type.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- sensitive to freezinge0
- Direction
- more sensitivee0
- Qualifier
- beforee0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are more sensitive to freezing, both before and after acclimation, than the wild-type plants”
t0“An Arabidopsis”
Claim · model-written summary
hos9-1 mutants were more sensitive to freezing after acclimation than wild type.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- sensitive to freezinge0
- Direction
- more sensitivee0
- Qualifier
- after acclimatione0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are more sensitive to freezing, both before and after acclimation, than the wild-type plants”
t0“An Arabidopsis”
Claim · model-written summary
HOS9 encodes a putative nuclear homeodomain transcription factor.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- HOS9e0
- Outcome
- putative homeodomain transcription factor that is localized to the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The HOS9 gene encodes a putative homeodomain transcription factor that is localized to the nucleus”
t0“An Arabidopsis”
Claim · model-written summary
HOS9 is constitutively expressed and not further induced by cold stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- cold stresse0
- Gene
- HOS9e0
- Outcome
- HOS9 is constitutively expressede0
- Direction
- not further inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HOS9 is constitutively expressed and not further induced by cold stress”
t0“An Arabidopsis”
Claim · model-written summary
Cold treatment raised endogenous RD29A transcript levels more in hos9-1 than in wild type.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Cold treatmente0
- Gene
- RD29Ae0
- Outcome
- level of transcripts of the endogenous RD29Ae0
- Direction
- highere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold treatment increased the level of transcripts of the endogenous RD29A, and some other stress-responsive genes, to a higher level in hos9-1 than in wild-type plants”
t0“An Arabidopsis”
Claim · model-written summary
Cold treatment raised transcripts of other stress-responsive genes more in hos9-1 than in wild type.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Cold treatmente0
- Gene
- other stress-responsive genese0
- Outcome
- level of transcriptse0
- Direction
- highere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cold treatment increased the level of transcripts of the endogenous RD29A, and some other stress-responsive genes, to a higher level in hos9-1 than in wild-type plants”
t0“An Arabidopsis”
Claim · model-written summary
The hos9-1 mutation did not alter the cold response of CBF genes.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- hos9-1 mutatione0
- Gene
- C repeat/dehydration responsive element-binding factor (CBF) transcription factor genese0
- Outcome
- response to colde0
- Direction
- did not have their response to cold alterede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the C repeat/dehydration responsive element-binding factor (CBF) transcription factor genes that mediate a part of cold acclimation in Arabidopsis did not have their response to cold altered by the hos9-1 mutation”
Claim · model-written summary
None of the genes affected by hos9-1 were controlled by the CBF family.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CBF familye0
- Outcome
- none of the genes affected by the hos9-1 mutation are controlled by the CBF familye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“none of the genes affected by the hos9-1 mutation are controlled by the CBF family”
t0“An Arabidopsis”
Claim · model-written summary
The authors infer that HOS9 supports growth, development and some freezing tolerance through CBF-independent gene activity.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- HOS9e0
- Outcome
- plant growth and development, and for a part of freezing tolerancee0
- Qualifier
- affecting the activity of genes independent of the CBF pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HOS9 is important for plant growth and development, and for a part of freezing tolerance, by affecting the activity of genes independent of the CBF pathway”
t0“An Arabidopsis”
The same questions in other papers
cold · molecular: expression · ArabidopsisPositive and negative results
20 papers in the same direction as this paper · 18 in the opposite direction
- positive
- no effect
- negative
35 papers more in this group
See all 39 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.