Abstract
The studies on calcium sensor calcineurin B-like protein (CBL) and CBL interacting protein kinases (CIPK) are limited to Arabidopsis and rice and their functional role is only beginning to emerge. Here, we present cloning and characterization of a protein kinase (PsCIPK) from a legume, pea, with novel properties. The PsCIPK gene is intronless and encodes a protein that showed partial homology to the members of CIPK family. The recombinant PsCIPK protein was autophosphorylated at Thr residue(s). Immunoprecipitation and yeast two-hybrid analysis showed direct interaction of PsCIPK with PsCBL, whose cDNA and genomic DNA were also cloned in this study. PsCBL showed homology to AtCBL3 and contained calcium-binding activity. We demonstrate for the first time that PsCBL is phosphorylated at its Thr residue(s) by PsCIPK. Immunofluorescence/confocal microscopy showed that PsCBL is exclusively localized in the cytosol, whereas PsCIPK is localized in the cytosol and the outer membrane. The exposure of plants to NaCl, cold and wounding co-ordinately upregulated the expression of PsCBL and PsCIPK genes. The transcript levels of both genes were also coordinately stimulated in response to calcium and salicylic acid. However, drought and abscisic acid had no effect on the expression of these genes. These studies show the ubiquitous presence of CBL/CIPK in higher plants and enhance our understanding of their role in abiotic and biotic stress signalling.
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Claims 16
Claim · model-written summary
Pea PsCIPK was cloned and characterized.
- Taxon
- pea
Lathyrus oleraceuse0 - Gene
- PsCIPKe0
- Outcome
- cloning and characterizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we present cloning and characterization of a protein kinase (PsCIPK) from a legume, pea, with novel properties.”
Claim · model-written summary
The PsCIPK gene is intronless and encodes a protein partially homologous to CIPK family members.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- PsCIPKe0
- Outcome
- intronless and encodes a protein that showed partial homologye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PsCIPK gene is intronless and encodes a protein that showed partial homology to the members of CIPK family.”
t0“Pisum sativum”
Claim · model-written summary
Recombinant PsCIPK autophosphorylated at threonine residues.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- PsCIPKe0
- Outcome
- autophosphorylated at Thr residue(s)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recombinant PsCIPK protein was autophosphorylated at Thr residue(s).”
t0“Pisum sativum”
Claim · model-written summary
PsCIPK directly interacted with PsCBL.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- PsCIPKe0
- Outcome
- direct interaction of PsCIPK with PsCBLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immunoprecipitation and yeast two-hybrid analysis showed direct interaction of PsCIPK with PsCBL, whose cDNA and genomic DNA were also cloned in this study.”
t0“Pisum sativum”
Claim · model-written summary
PsCBL cDNA and genomic DNA were cloned.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- PsCBLe0
- Outcome
- cDNA and genomic DNA were also clonede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsCBL, whose cDNA and genomic DNA were also cloned in this study.”
t0“Pisum sativum”
Claim · model-written summary
PsCBL showed homology to AtCBL3 and calcium-binding activity.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- PsCBLe0
- Outcome
- homology to AtCBL3 and contained calcium-binding activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsCBL showed homology to AtCBL3 and contained calcium-binding activity.”
t0“Pisum sativum”
Claim · model-written summary
PsCIPK phosphorylated PsCBL at threonine residues.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- PsCBLe0
- Outcome
- phosphorylated at its Thr residue(s) by PsCIPKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsCBL is phosphorylated at its Thr residue(s) by PsCIPK.”
t0“Pisum sativum”
Claim · model-written summary
PsCBL localized exclusively to the cytosol.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- PsCBLe0
- Outcome
- exclusively localized in the cytosole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsCBL is exclusively localized in the cytosol”
t0“Pisum sativum”
Claim · model-written summary
PsCIPK localized to the cytosol and outer membrane.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Gene
- PsCIPKe0
- Outcome
- localized in the cytosol and the outer membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsCIPK is localized in the cytosol and the outer membrane.”
t0“Pisum sativum”
Claim · model-written summary
NaCl upregulated PsCBL and PsCIPK expression.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- NaCle0
- Outcome
- expression of PsCBL and PsCIPK genese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The exposure of plants to NaCl, cold and wounding co-ordinately upregulated the expression of PsCBL and PsCIPK genes.”
t0“Pisum sativum”
Claim · model-written summary
Cold upregulated PsCBL and PsCIPK expression.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- colde0
- Outcome
- expression of PsCBL and PsCIPK genese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The exposure of plants to NaCl, cold and wounding co-ordinately upregulated the expression of PsCBL and PsCIPK genes.”
t0“Pisum sativum”
Claim · model-written summary
Wounding upregulated PsCBL and PsCIPK expression.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- woundinge0
- Outcome
- expression of PsCBL and PsCIPK genese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The exposure of plants to NaCl, cold and wounding co-ordinately upregulated the expression of PsCBL and PsCIPK genes.”
t0“Pisum sativum”
Claim · model-written summary
Calcium stimulated PsCBL and PsCIPK transcript levels.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- calciume0
- Outcome
- transcript levels of both genese0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript levels of both genes were also coordinately stimulated in response to calcium and salicylic acid.”
t0“Pisum sativum”
Claim · model-written summary
Salicylic acid stimulated PsCBL and PsCIPK transcript levels.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- salicylic acide0
- Outcome
- transcript levels of both genese0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript levels of both genes were also coordinately stimulated in response to calcium and salicylic acid.”
t0“Pisum sativum”
Claim · model-written summary
Drought did not affect expression of PsCBL and PsCIPK.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- droughte0
- Outcome
- expression of these genese0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, drought and abscisic acid had no effect on the expression of these genes.”
t0“Pisum sativum”
Claim · model-written summary
Abscisic acid did not affect expression of PsCBL and PsCIPK.
- Taxon
- Pisum sativumLathyrus oleraceust0
- Treatment
- abscisic acide0
- Outcome
- expression of these genese0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, drought and abscisic acid had no effect on the expression of these genes.”
t0“Pisum sativum”
The same questions in other papers
sodium chloride · quality: protein · Lathyrus oleraceus (pea)Positive and negative results
1 in the opposite direction
- negative
salicylic acid · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
mechanical · quality: protein · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
2 papers more in this group
See all 6 papers →drought · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →cold · quality: protein · Lathyrus oleraceus (pea)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.