Abstract
Late-embryogenesis abundant (LEA) proteins are hydrophilic proteins that accumulate to a high level in desiccation-tolerant tissues and are thus prominent in seeds. They are expected to play a protective role during dehydration; however, functional evidence is scarce. We identified a LEA protein of group 3 (PsLEAm) that was localized within the matrix space of pea (Pisum sativum) seed mitochondria. PsLEAm revealed typical LEA features such as high hydrophilicity and repeated motifs, except for the N-terminal transit peptide. Most of the highly charged protein was predicted to fold into amphiphilic alpha-helixes. PsLEAm was expressed during late seed development and remained in the dry seed and throughout germination. Application of the stress hormone abscisic acid was found to reinduce the expression of PsLEAm transcripts during germination. PsLEAm could not be detected in vegetative tissues; however, its expression could be reinduced in leaves by severe water stress. The recombinant PsLEAm was shown to protect two mitochondrial matrix enzymes, fumarase and rhodanese, during drying in an in vitro assay. The overall results constitute, to our knowledge, the first characterization of a LEA protein in mitochondria and experimental evidence for a beneficial role of a LEA protein with respect to proteins during desiccation.
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Claims 8
Claim · model-written summary
PsLEAm was localized to the matrix of pea seed mitochondria.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- seed mitochondriae0
- Gene
- PsLEAme0
- Outcome
- localized within the matrix spacee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a LEA protein of group 3 (PsLEAm) that was localized within the matrix space of pea (Pisum sativum) seed mitochondria”
Claim · model-written summary
PsLEAm had high hydrophilicity and repeated motifs.
- Taxon
- peaLathyrus oleraceust0
- Gene
- PsLEAme0
- Outcome
- high hydrophilicity and repeated motifse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsLEAm revealed typical LEA features such as high hydrophilicity and repeated motifs, except for the N-terminal transit peptide”
t0“Pea Seed Mitochondria”
Claim · model-written summary
Most PsLEAm was predicted to form amphiphilic alpha-helixes.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- predicted to fold into amphiphilic alpha-helixese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most of the highly charged protein was predicted to fold into amphiphilic alpha-helixes”
t0“Pea Seed Mitochondria”
Claim · model-written summary
PsLEAm was expressed late in seed development and remained through germination.
- Taxon
- PeaLathyrus oleraceust0
- Gene
- PsLEAme0
- Outcome
- expressed during late seed development and remained in the dry seed and throughout germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsLEAm was expressed during late seed development and remained in the dry seed and throughout germination”
t0“Pea Seed Mitochondria”
Claim · model-written summary
Abscisic acid reinduced PsLEAm transcripts during germination.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- germinatione0
- Treatment
- abscisic acide0
- Gene
- PsLEAme0
- Outcome
- expression of PsLEAm transcriptse0
- Direction
- reinducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of the stress hormone abscisic acid was found to reinduce the expression of PsLEAm transcripts during germination”
t0“Pea Seed Mitochondria”
Claim · model-written summary
PsLEAm was undetected in vegetative tissues.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- vegetative tissuese0
- Gene
- PsLEAme0
- Outcome
- could not be detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsLEAm could not be detected in vegetative tissues”
t0“Pea Seed Mitochondria”
Claim · model-written summary
Severe water stress reinduced PsLEAm expression in leaves.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- leavese0
- Treatment
- severe water stresse0
- Outcome
- expressione0
- Direction
- reinducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression could be reinduced in leaves by severe water stress”
t0“Pea Seed Mitochondria”
Claim · model-written summary
Recombinant PsLEAm protected fumarase and rhodanese during drying in vitro.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- recombinant PsLEAme0
- Gene
- PsLEAme0
- Outcome
- protect two mitochondrial matrix enzymes, fumarase and rhodanesee0
- Direction
- protecte0
- Qualifier
- during drying in an in vitro assaye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recombinant PsLEAm was shown to protect two mitochondrial matrix enzymes, fumarase and rhodanese, during drying in an in vitro assay”
t0“Pea Seed Mitochondria”
The same questions in other papers
drought · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 3 in the opposite direction
- no effect
- negative
- no effect
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.