Abstract
Wounding of aged, previously-excised pea epicotyl segments by removal of the basal 1–2 mm resulted in a rapid (beginning within 15 min) recruitment of monosomes on to polysomes and an even more rapid (maximal between 6–12 min) inhibition of protein synthesis in the remaining tissue. This inhibition of protein synthesis in vivo did not appear to be an artefact caused by the removal of highly active tissue (e.g., callus, contaminating bacteria), since wounds inflicted at a site distant from the region analyzed still elicited the response, and protein synthesis in the 1–2 mm slices (normally discarded) was inhibited even more strongly than it was in the remaining tissue. The proportion of radioactive methionine in nascent chains (bound to polysomes) increased, while the production of completed polypeptides decreased, after wounding. Cycloheximide, a known inhibitor of the ribosome translocation/release process mimicked some of the effects of wounding. We interpret the results to indicate that the initial effect of wounding is to inhibit translation by inhibiting the ribosome translocation/release process, whereas the subsequent recovery in protein synthesis is brought about partly by a recovery in ribosome translocation/release and partly by enhanced initiation.
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Claims 9
Claim · model-written summary
Wounding rapidly recruited monosomes onto polysomes in pea epicotyls.
- Taxon
- pea
Lathyrus oleraceuse0 - Organ / stage
- epicotyl segmentse0
- Treatment
- Woundinge0
- Outcome
- recruitment of monosomes on to polysomese0
- Direction
- recruitmente0
- Value
- within 15 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wounding of aged, previously-excised pea epicotyl segments by removal of the basal 1–2 mm resulted in a rapid (beginning within 15 min) recruitment of monosomes on to polysomes”
Claim · model-written summary
Wounding rapidly inhibited protein synthesis in the remaining pea tissue.
- Taxon
- peaLathyrus oleraceuse1
- Organ / stage
- epicotyl segmentse1
- Treatment
- Woundinge1
- Outcome
- protein synthesise0
- Direction
- inhibitione0
- Value
- 6–12 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an even more rapid (maximal between 6–12 min) inhibition of protein synthesis in the remaining tissue”
e1“Wounding of aged, previously-excised pea epicotyl segments”
Claim · model-written summary
Distant wounds also inhibited protein synthesis in pea epicotyl tissue.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- wounds inflicted at a site distant from the region analyzede0
- Outcome
- protein synthesise1
- Direction
- inhibitione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“wounds inflicted at a site distant from the region analyzed still elicited the response”
e1“This inhibition of protein synthesis in vivo”
t0“Pea Epicotyls”
Claim · model-written summary
Protein synthesis was more strongly inhibited in removed pea slices than in remaining tissue.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- 1–2 mm slicese0
- Treatment
- Woundinge1
- Outcome
- protein synthesise0
- Direction
- inhibited even more stronglye0
- Comparator
- remaining tissuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protein synthesis in the 1–2 mm slices (normally discarded) was inhibited even more strongly than it was in the remaining tissue”
e1“Wounding of aged, previously-excised pea epicotyl segments”
t0“Pea Epicotyls”
Claim · model-written summary
Wounding increased radioactive methionine in nascent chains of pea epicotyls.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- woundinge1
- Outcome
- proportion of radioactive methionine in nascent chainse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The proportion of radioactive methionine in nascent chains (bound to polysomes) increased”
e1“after wounding”
t0“Pea Epicotyls”
Claim · model-written summary
Wounding decreased production of completed polypeptides in pea epicotyls.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- woundinge0
- Outcome
- production of completed polypeptidese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the production of completed polypeptides decreased, after wounding”
t0“Pea Epicotyls”
Claim · model-written summary
Cycloheximide mimicked some effects of wounding in pea epicotyls.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- Cycloheximidee0
- Outcome
- mimicked some of the effects of woundinge0
- Comparator
- woundinge0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cycloheximide, a known inhibitor of the ribosome translocation/release process mimicked some of the effects of wounding”
t0“Pea Epicotyls”
Claim · model-written summary
The authors interpret wounding as initially inhibiting translation through ribosome translocation and release.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- woundinge0
- Outcome
- translatione0
- Qualifier
- ribosome translocation/release processe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the initial effect of wounding is to inhibit translation by inhibiting the ribosome translocation/release process”
t0“Pea Epicotyls”
Claim · model-written summary
The authors attribute recovery of pea protein synthesis partly to translocation and release recovery and enhanced initiation.
- Taxon
- PeaLathyrus oleraceust0
- Outcome
- recovery in protein synthesise0
- Qualifier
- recovery in ribosome translocation/release and partly by enhanced initiatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the subsequent recovery in protein synthesis is brought about partly by a recovery in ribosome translocation/release and partly by enhanced initiation”
t0“Pea Epicotyls”
The same questions in other papers
mechanical · quality: protein · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →mechanical · yield · Lathyrus oleraceus (pea)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.