Abstract
RNA and protein metabolism were greatly altered during aging, as well as after wounding and auxin treatment of aged pea epicotyls (Pisum sativum L. cv. Alaska). Aging was initiated by removal of the hooks and plumules from actively-growing plants. Lanolin was applied to the cut ends and the plants were allowed to age for up to four days. During this aging period several marked, degenerative events occurred. Ribosomal RNA and messenger RNA contents declined, polyribosomes disaggregated and the protein synthesizing capacity of the polysomes decreased. Two-dimensional, silver-stained protein patterns revealed that aging altered the relative amounts of specific cellular proteins accumulated in vivo. In addition, polypeptide patterns generated by cell-free translation of total and polysomal RNA, isolated from unaged and aged tissues, showed dramatic modifications. Wounding treatment of aged pea epicotyls stimulated rapid polyribosome formation. Increases in RNA content and protein synthesizing capacity were the result of changes in transcription and translation, although translation was affected to a much greater extent. Minor differences were seen in the two-dimensional, silver-stained protein patterns for wounded tissue. Wounding caused changes in the messenger RNA populations as determined by two-dimensional gel electrophoresis of cell-free translation products which were substantially different from the control. Auxin treatment of aged pea epicotyls increased the poly(adenylic acid) content of free and membrane-bound polyribosomes. The increased amount of messenger RNA in these polysomes was not the result of a shift of non-polysomal message into polysomes. The increase in poly(adenylic acid) content was exceeded by an increase in the protein synthesizing capacity of the polysomes. In contrast to wounding, auxin appeared to affect transcription to a greater extent than translation. Auxin caused only minor changes in two-dimensional, silver-stained protein maps, whereas extensive alterations were observed for polypeptides synthesized in vitro.
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Claims 15
Claim · model-written summary
Aging decreased ribosomal RNA and messenger RNA contents of pea epicotyls.
- Taxon
- Pisum sativum L.
Lathyrus oleraceuse0 - Material
- cv. Alaskae0
- Organ / stage
- epicotylse0
- Treatment
- aginge1
- Outcome
- Ribosomal RNA and messenger RNA contentse2
- Direction
- declinede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“RNA and protein metabolism were greatly altered during aging”
e2“Ribosomal RNA and messenger RNA contents declined”
Claim · model-written summary
Aging disaggregated polyribosomes in pea epicotyls.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- aginge1
- Outcome
- polyribosomese3
- Direction
- disaggregatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“RNA and protein metabolism were greatly altered during aging”
e3“polyribosomes disaggregated”
Claim · model-written summary
Aging decreased the protein synthesizing capacity of polysomes in pea epicotyls.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- aginge1
- Outcome
- protein synthesizing capacity of the polysomese4
- Direction
- decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“RNA and protein metabolism were greatly altered during aging”
e4“the protein synthesizing capacity of the polysomes decreased”
Claim · model-written summary
Aging altered the relative amounts of specific cellular proteins accumulated in vivo.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- aginge5
- Outcome
- relative amounts of specific cellular proteinse5
- Direction
- alterede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e5“aging altered the relative amounts of specific cellular proteins accumulated in vivo”
Claim · model-written summary
Aged tissue showed dramatically modified cell-free translation polypeptide patterns relative to unaged tissue.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- aginge1
- Outcome
- polypeptide patterns generated by cell-free translatione6
- Direction
- modificationse6
- Comparator
- unagede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“RNA and protein metabolism were greatly altered during aging”
e6“polypeptide patterns generated by cell-free translation of total and polysomal RNA, isolated from unaged and aged tissues, showed dramatic modifications”
Claim · model-written summary
Wounding of aged pea epicotyls stimulated polyribosome formation.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- Wounding treatmente7
- Outcome
- polyribosome formatione7
- Direction
- stimulatede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e7“Wounding treatment of aged pea epicotyls stimulated rapid polyribosome formation”
Claim · model-written summary
Increases in RNA content and protein synthesizing capacity are explained by changes in transcription and translation.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Outcome
- RNA content and protein synthesizing capacitye1
- Qualifier
- changes in transcription and translatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“Increases in RNA content and protein synthesizing capacity were the result of changes in transcription and translation”
Claim · model-written summary
Wounding caused only minor differences in two-dimensional protein patterns of pea epicotyls.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- wounded tissuee1
- Outcome
- two-dimensional, silver-stained protein patternse1
- Direction
- Minor differencese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“Minor differences were seen in the two-dimensional, silver-stained protein patterns for wounded tissue”
Claim · model-written summary
Wounding changed messenger RNA populations relative to the control.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- Woundinge1
- Outcome
- messenger RNA populationse1
- Direction
- changese1
- Comparator
- controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“Wounding caused changes in the messenger RNA populations as determined by two-dimensional gel electrophoresis of cell-free translation products which were substantially different from the control”
Claim · model-written summary
Auxin increased the poly(adenylic acid) content of free and membrane-bound polyribosomes of aged pea epicotyls.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- Auxin treatmente1
- Outcome
- poly(adenylic acid) content of free and membrane-bound polyribosomese1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“Auxin treatment of aged pea epicotyls increased the poly(adenylic acid) content of free and membrane-bound polyribosomes”
Claim · model-written summary
The increased messenger RNA in polysomes after auxin was not due to a shift of non-polysomal message into polysomes.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Outcome
- increased amount of messenger RNA in these polysomese1
- Qualifier
- was not the result of a shift of non-polysomal message into polysomese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“The increased amount of messenger RNA in these polysomes was not the result of a shift of non-polysomal message into polysomes”
Claim · model-written summary
The increase in protein synthesizing capacity of polysomes exceeded the increase in poly(adenylic acid) content after auxin treatment.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- Auxin treatmente1
- Outcome
- protein synthesizing capacity of the polysomese2
- Direction
- an increasee2
- Comparator
- increase in poly(adenylic acid) contente2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“Auxin treatment of aged pea epicotyls increased the poly(adenylic acid) content of free and membrane-bound polyribosomes”
e2“The increase in poly(adenylic acid) content was exceeded by an increase in the protein synthesizing capacity of the polysomes”
Claim · model-written summary
Auxin appears to affect transcription more than translation in pea epicotyls.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- auxine1
- Outcome
- transcriptione1
- Direction
- greater extente1
- Comparator
- translatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“auxin appeared to affect transcription to a greater extent than translation”
Claim · model-written summary
Auxin caused only minor changes in two-dimensional, silver-stained protein maps of pea epicotyls.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- Auxine1
- Outcome
- two-dimensional, silver-stained protein mapse1
- Direction
- minor changese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“Auxin caused only minor changes in two-dimensional, silver-stained protein maps”
Claim · model-written summary
Auxin was followed by extensive alterations in polypeptides synthesized in vitro.
- Taxon
- Pisum sativum L.Lathyrus oleraceuse0
- Treatment
- Auxine1
- Outcome
- polypeptides synthesized in vitroe2
- Direction
- extensive alterationse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“aged pea epicotyls (Pisum sativum L. cv. Alaska)”
e1“Auxin caused only minor changes in two-dimensional, silver-stained protein maps”
e2“extensive alterations were observed for polypeptides synthesized in vitro”
The same questions in other papers
mechanical · quality: protein · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →class · quality: protein · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.