Abstract
Stress phytohormones - salicylic and jasmonic acids - participate in the plant defense response. The increase in the content of these compounds during plant infection by pathogens leads to the activation of signaling pathways, ultimately resulting in changes in gene expression and protein synthesis, including a group of pathogenesis-related (PR) proteins. Phytohormone-dependent so-called marker genes, are used to assess the activation of these signaling pathways. In this study, PR-1 genes, which are markers for salicylic acid signaling and expressed in pea roots, were identified and characterized, and the effects of salicylic acid and methyl jasmonate on their expression were analyzed. It was shown that in pea roots, PR-1, encoded by the Psat1g156240 gene, is among the most significantly expressed genes in the control. Salicylic acid did not cause a change in the expression of this gene; however, it was induced by methyl jasmonate after 24 h. Analysis of the expression of other genes encoding PR-1 proteins showed that salicylate had no effect on their expression after 24 and 72 h. Analysis of the expression of genes encoding chitinases and chitinase-like proteins showed that the former do not exhibit specificity in response to the salicylate and methyl jasmonate, except for the Psat1g131280, the expression of which increased after 24 and 72 h of treatment with methyl jasmonate. Genes Psat1g147600, Psat1g147560, Psat1g149120 encoding chitinase-like proteins, were barely expressed in pea roots in control, and were specifically induced by salicylic acid. β-1,3-glucanase genes were not induced in roots by the studied phytohormones. The obtained results allowed to reveal specific genes including chitinase-like proteins, the expression of which is salicylate-inducible. These genes can be used for the assessment of the activation of the salicylate-dependent signaling pathway in pea roots.
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Claims 10
Claim · model-written summary
In pea roots the PR-1 gene Psat1g156240 is among the most significantly expressed genes in the control.
- Taxon
- pea
Lathyrus oleraceuse1 - Gene
- Psat1g156240e1
- Outcome
- among the most significantly expressed genese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in pea roots, PR-1, encoded by the Psat1g156240 gene, is among the most significantly expressed genes in the control”
Claim · model-written summary
Salicylic acid did not change expression of the PR-1 gene Psat1g156240 in pea roots.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- Salicylic acide2
- Gene
- Psat1g156240e1
- Outcome
- expressione2
- Direction
- did not cause a changee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in pea roots, PR-1, encoded by the Psat1g156240 gene, is among the most significantly expressed genes in the control”
e2“Salicylic acid did not cause a change in the expression of this gene”
Claim · model-written summary
Methyl jasmonate induced expression of the PR-1 gene Psat1g156240 in pea roots after 24 h.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- methyl jasmonatee2
- Gene
- Psat1g156240e1
- Outcome
- expressione2
- Direction
- inducede2
- Qualifier
- after 24 he2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in pea roots, PR-1, encoded by the Psat1g156240 gene, is among the most significantly expressed genes in the control”
e2“Salicylic acid did not cause a change in the expression of this gene; however, it was induced by methyl jasmonate after 24 h”
Claim · model-written summary
Salicylate had no effect on expression of the other genes encoding PR-1 proteins in pea roots after 24 and 72 h.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- salicylatee2
- Gene
- genes encoding PR-1 proteinse2
- Outcome
- expressione2
- Direction
- no effecte2
- Qualifier
- after 24 and 72 he2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in pea roots, PR-1, encoded by the Psat1g156240 gene, is among the most significantly expressed genes in the control”
e2“Analysis of the expression of other genes encoding PR-1 proteins showed that salicylate had no effect on their expression after 24 and 72 h”
Claim · model-written summary
Genes encoding chitinases did not exhibit specificity in response to salicylate and methyl jasmonate in pea roots, apart from one gene.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- salicylate and methyl jasmonatee2
- Gene
- genes encoding chitinasese2
- Outcome
- specificity in responsee2
- Direction
- do not exhibite2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in pea roots, PR-1, encoded by the Psat1g156240 gene, is among the most significantly expressed genes in the control”
e2“genes encoding chitinases and chitinase-like proteins showed that the former do not exhibit specificity in response to the salicylate and methyl jasmonate”
Claim · model-written summary
Expression of the chitinase gene Psat1g131280 increased after 24 and 72 h of methyl jasmonate treatment in pea roots.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- methyl jasmonatee2
- Gene
- Psat1g131280e2
- Outcome
- expressione2
- Direction
- increasede2
- Qualifier
- after 24 and 72 he2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in pea roots, PR-1, encoded by the Psat1g156240 gene, is among the most significantly expressed genes in the control”
e2“the Psat1g131280, the expression of which increased after 24 and 72 h of treatment with methyl jasmonate”
Claim · model-written summary
The chitinase-like genes Psat1g147600, Psat1g147560 and Psat1g149120 were barely expressed in pea roots in the control.
- Taxon
- peaLathyrus oleraceuse1
- Gene
- Psat1g147600, Psat1g147560, Psat1g149120e1
- Outcome
- barely expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Genes Psat1g147600, Psat1g147560, Psat1g149120 encoding chitinase-like proteins, were barely expressed in pea roots in control”
Claim · model-written summary
The chitinase-like genes Psat1g147600, Psat1g147560 and Psat1g149120 were specifically induced by salicylic acid in pea roots.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- salicylic acide1
- Gene
- Psat1g147600, Psat1g147560, Psat1g149120e1
- Outcome
- specifically inducede1
- Direction
- inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Genes Psat1g147600, Psat1g147560, Psat1g149120 encoding chitinase-like proteins, were barely expressed in pea roots in control, and were specifically induced by salicylic acid”
Claim · model-written summary
β-1,3-glucanase genes were not induced in pea roots by the studied phytohormones.
- Taxon
- peaLathyrus oleraceuse1
- Treatment
- studied phytohormonese2
- Gene
- β-1,3-glucanase genese2
- Outcome
- β-1,3-glucanase genese2
- Direction
- not inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“in pea roots, PR-1, encoded by the Psat1g156240 gene, is among the most significantly expressed genes in the control”
e2“β-1,3-glucanase genes were not induced in roots by the studied phytohormones”
Claim · model-written summary
The salicylate-inducible genes, including chitinase-like protein genes, can be used to assess activation of the salicylate-dependent signaling pathway in pea roots.
- Taxon
- peaLathyrus oleraceuse1
- Outcome
- assessment of the activation of the salicylate-dependent signaling pathwaye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These genes can be used for the assessment of the activation of the salicylate-dependent signaling pathway in pea roots”
The same questions in other papers
salicylic acid · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
class · molecular: expression · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.