Abstract
Thiamine confers systemic acquired resistance (SAR) on susceptible plants through priming, leading to rapid counterattack against pathogen invasion and perturbation of disease progress. Priming reduces the metabolic cost required for constitutive expression of acquired resistance. To investigate the effects of priming by thiamine on defense-related responses, Arabidopsis (Arabidopsis thaliana) was treated with thiamine and effects of pathogen challenge on the production of active oxygen species, callose deposition, hypersensitive cell death, and pathogenesis-related 1 (PR1)/Phe ammonia-lyase 1 (PAL1) gene expression was analyzed. Thiamine did not induce cellular and molecular defense responses except for transient expression of PR1 per se; however, subsequent Pseudomonas syringae pv tomato challenge triggered pronounced cellular defense responses and advanced activation of PR1/PAL1 gene transcription. Thiamine treatment and subsequent pathogen invasion triggered hydrogen peroxide accumulation, callose induction, and PR1/PAL1 transcription activation in Arabidopsis mutants insensitive to jasmonic acid (jar1), ethylene (etr1), or abscisic acid (abi3-3), but not in plants expressing bacterial NahG and lacking regulation of SAR (npr1 [nonexpressor of PR genes 1]). Moreover, removal of hydrogen peroxide by catalase almost completely nullified cellular and molecular defense responses as well as SAR abolishing bacterial propagation within plants. Our results indicated that priming is an important cellular mechanism in SAR by thiamine and requires hydrogen peroxide and intact NPR1.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Thiamine alone caused transient PR1 expression but no other defense responses.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Thiaminee1
- Gene
- PR1e1
- Outcome
- cellular and molecular defense responsese1
- Direction
- did not inducee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“Thiamine did not induce cellular and molecular defense responses except for transient expression of PR1 per se”
Claim · model-written summary
Pathogen challenge after thiamine triggered pronounced cellular defense responses.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Pseudomonas syringae pv tomato challengee1
- Interactor
- Pseudomonas syringae pv tomato
Pseudomonas syringaee1pathogen - Outcome
- cellular defense responsese1
- Direction
- pronouncede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“subsequent Pseudomonas syringae pv tomato challenge triggered pronounced cellular defense responses and advanced activation of PR1/PAL1 gene transcription.”
Claim · model-written summary
Pathogen challenge after thiamine advanced PR1/PAL1 transcription.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Pseudomonas syringae pv tomato challengee1
- Interactor
- Pseudomonas syringae pv tomatoPseudomonas syringaee1pathogen
- Gene
- PR1/PAL1e1
- Outcome
- PR1/PAL1 gene transcriptione1
- Direction
- advancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“subsequent Pseudomonas syringae pv tomato challenge triggered pronounced cellular defense responses and advanced activation of PR1/PAL1 gene transcription.”
Claim · model-written summary
Defense priming remained in jar1, etr1 and abi3-3 mutants but was absent in NahG and npr1 plants.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- hydrogen peroxide accumulation, callose induction, and PR1/PAL1 transcription activatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thiamine treatment and subsequent pathogen invasion triggered hydrogen peroxide accumulation, callose induction, and PR1/PAL1 transcription activation in Arabidopsis mutants insensitive to jasmonic acid (jar1), ethylene (etr1), or abscisic acid (abi3-3), but not in plants expressing bacterial NahG and lacking regulation of SAR (npr1 [nonexpressor of PR genes 1]).”
Claim · model-written summary
Catalase removal of hydrogen peroxide nearly nullified cellular and molecular defense responses.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- removal of hydrogen peroxide by catalasee1
- Outcome
- cellular and molecular defense responsese1
- Direction
- nullifiede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“removal of hydrogen peroxide by catalase almost completely nullified cellular and molecular defense responses as well as SAR abolishing bacterial propagation within plants.”
Claim · model-written summary
Catalase removal of hydrogen peroxide nearly nullified SAR.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- removal of hydrogen peroxide by catalasee1
- Outcome
- SARe1
- Direction
- nullifiede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“removal of hydrogen peroxide by catalase almost completely nullified cellular and molecular defense responses as well as SAR abolishing bacterial propagation within plants.”
Claim · model-written summary
Authors infer that thiamine priming requires hydrogen peroxide and intact NPR1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- NPR1e1
- Outcome
- SAR by thiaminee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis (Arabidopsis thaliana)”
e1“priming is an important cellular mechanism in SAR by thiamine and requires hydrogen peroxide and intact NPR1.”
The same questions in other papers
Solanum lycopersicum · disease · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
hydrogen peroxide · disease · Arabidopsis thaliana (thale cress)Positive and negative results
2 in the opposite direction
- positive
- positive
Solanum lycopersicum · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
Pseudomonas syringae · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Pseudomonas syringae · disease · Arabidopsis thaliana (thale cress)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
3 papers more in this group
See all 7 papers →infection · disease · Arabidopsis thaliana (thale cress)Positive and negative results
6 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
6 papers more in this group
See all 10 papers →infection · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
17 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- negative
20 papers more in this group
See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.