Abstract
We isolated and characterized a 2.8-kb, full-length, Arabidopsis thaliana cDNA clone encoding a lipoxygenase. DNA sequence analysis showed that the deduced amino acid sequence of the Arabidopsis protein is 72 to 78% similar to that of legume seed lipoxygenases. DNA blot analysis indicated that Arabidopsis contains a single gene, LOX1, with appreciable homology to the cDNA clone. RNA blot analysis showed that the LOX1 gene is expressed in Arabidopsis leaves, roots, inflorescences, and young seedlings. LOX1 expression levels were highest in roots and young seedlings. In mature plants, LOX1 mRNA levels increased upon treatment with the stress-related hormones abscisic acid and methyl jasmonate and remained high for at least 96 h. Expression of the LOX1 gene was examined following infiltration of leaves with virulent (Psm ES4326) and avirulent (Pst MM1065) strains of Pseudomonas syringae. LOX1 mRNA levels were induced approximately 6-fold by both virulent and avirulent strains; however, the response to avirulent strains was much more rapid. Infiltration of leaves with Pst MM1065 resulted in maximal induction within 12 h, whereas maximal induction by Psm ES4326 did not occur until 48 h. When a cloned avr gene, avrRpt2, was transferred to Psm ES4326, LOX1 mRNA accumulated in a pattern similar to that observed for the avirulent strain Pst MM1065.
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Claims 10
Claim · model-written summary
A 2.8-kb full-length Arabidopsis thaliana cDNA clone encoding a lipoxygenase was isolated and characterized.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Outcome
- cDNA clone encoding a lipoxygenasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We isolated and characterized a 2.8-kb, full-length, Arabidopsis thaliana cDNA clone encoding a lipoxygenase”
Claim · model-written summary
The deduced Arabidopsis protein sequence is 72 to 78% similar to legume seed lipoxygenases.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- deduced amino acid sequencee0
- Value
- 72 to 78%e0
- Comparator
- legume seed lipoxygenasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“the deduced amino acid sequence of the Arabidopsis protein is 72 to 78% similar to that of legume seed lipoxygenases”
Claim · model-written summary
Arabidopsis contains a single gene, LOX1, with appreciable homology to the cDNA clone.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- LOX1e0
- Outcome
- a single genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Arabidopsis contains a single gene, LOX1”
Claim · model-written summary
The LOX1 gene is expressed in Arabidopsis leaves, roots, inflorescences and young seedlings.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- leaves, roots, inflorescences, and young seedlingse0
- Gene
- LOX1e0
- Outcome
- expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the LOX1 gene is expressed in Arabidopsis leaves, roots, inflorescences, and young seedlings”
Claim · model-written summary
LOX1 expression levels were highest in roots and young seedlings.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- roots and young seedlingse0
- Gene
- LOX1e0
- Outcome
- LOX1 expression levelse0
- Qualifier
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“LOX1 expression levels were highest in roots and young seedlings”
Claim · model-written summary
In mature Arabidopsis plants LOX1 mRNA levels increased after treatment with abscisic acid and methyl jasmonate and remained high for at least 96 h.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- abscisic acid and methyl jasmonatee0
- Gene
- LOX1e0
- Outcome
- LOX1 mRNA levelse0
- Direction
- increasede0
- Qualifier
- remained high for at least 96 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“LOX1 mRNA levels increased upon treatment with the stress-related hormones abscisic acid and methyl jasmonate and remained high for at least 96 h”
Claim · model-written summary
Infiltration of Arabidopsis leaves with virulent or avirulent Pseudomonas syringae strains induced LOX1 mRNA levels approximately 6-fold.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- infiltration of leaves with virulent (Psm ES4326) and avirulent (Pst MM1065) strains of Pseudomonas syringaee1
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee1pathogen - Outcome
- LOX1 mRNA levelse0
- Direction
- inducede0
- Value
- approximately 6-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“LOX1 mRNA levels were induced approximately 6-fold by both virulent and avirulent strains”
e1“infiltration of leaves with virulent (Psm ES4326) and avirulent (Pst MM1065) strains of Pseudomonas syringae”
Claim · model-written summary
Infiltration with the avirulent strain Pst MM1065 gave maximal LOX1 induction within 12 h.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Infiltration of leaves with Pst MM1065e0
- Interactor
- Pst MM1065e0pathogen
- Outcome
- maximal inductione0
- Direction
- maximal inductione0
- Value
- within 12 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“Infiltration of leaves with Pst MM1065 resulted in maximal induction within 12 h”
Claim · model-written summary
With the virulent strain Psm ES4326, maximal LOX1 induction did not occur until 48 h.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Psm ES4326e0
- Interactor
- Psm ES4326e0pathogen
- Outcome
- maximal inductione0
- Direction
- maximal inductione0
- Value
- 48 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“maximal induction by Psm ES4326 did not occur until 48 h”
Claim · model-written summary
When avrRpt2 was transferred to Psm ES4326, LOX1 mRNA accumulated in a pattern similar to that with the avirulent strain Pst MM1065.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- cloned avr gene, avrRpt2, was transferred to Psm ES4326e0
- Interactor
- Psm ES4326e0pathogen
- Second interactor
- Pst MM1065e0pathogen
- Gene
- LOX1e0
- Outcome
- LOX1 mRNAe0
- Direction
- accumulatede0
- Comparator
- avirulent strain Pst MM1065e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“When a cloned avr gene, avrRpt2, was transferred to Psm ES4326, LOX1 mRNA accumulated in a pattern similar to that observed for the avirulent strain Pst MM1065”
The same questions in other papers
Pseudomonas syringae · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.