Abstract
To discover which components of plant defense responses make significant contributions to limiting pathogen attack, we screened a mutagenized population of Arabidopsis thaliana for individuals that exhibit increased susceptibility to the moderately virulent bacterial pathogen Pseudomonas syringae pv. maculicola ES4326 (Psm ES4326). The 12 enhanced disease susceptibility (eds) mutants isolated included alleles of two genes involved in phytoalexin biosynthesis (pad2, which had been identified previously, and pad4, which had not been identified previously), two alleles of the previously identified npr1 gene, which affects expression of other defense genes, and alleles of seven previously unidentified genes of unknown function. The npr1 mutations caused greatly reduced expression of the PR1 gene in response to PsmES4326 infection, but had little effect on expression of two other defense genes, BGL2 and PR5, suggesting that PR1 expression may be important for limiting growth of PsmES4326. While direct screens for mutants with quantitative pathogen-susceptibility phenotypes have not been reported previously, our finding that mutants isolated in this way include those affected in known defense responses supports the notion that this type of screening strategy allows genetic dissection of the roles of various plant defense responses in disease resistance.
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Claims 7
Claim · model-written summary
The screen isolated 12 enhanced-susceptibility mutants.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Interactor
- Pseudomonas syringae pv. maculicola ES4326Pseudomonas syringae pv. maculicolae2pathogen
- Outcome
- enhanced disease susceptibility (eds) mutantse0
- Value
- 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 12 enhanced disease susceptibility (eds) mutants isolated included alleles of two genes involved in phytoalexin biosynthesis (pad2, which had been identified previously, and pad4, which had not been identified previously), two alleles of the previously identified npr1 gene, which affects expression of other defense genes, and alleles of seven previously unidentified genes of unknown function.”
e1“Arabidopsis thaliana”
e2“Pseudomonas syringae pv. maculicola ES4326”
Claim · model-written summary
The mutants included pad2 and newly identified pad4 alleles.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- pad2e0
- Outcome
- alleles of two genes involved in phytoalexin biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alleles of two genes involved in phytoalexin biosynthesis (pad2, which had been identified previously, and pad4, which had not been identified previously)”
e1“Arabidopsis thaliana”
Claim · model-written summary
The screen found two npr1 alleles.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- npr1e0
- Outcome
- two allelese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two alleles of the previously identified npr1 gene”
e1“Arabidopsis thaliana”
Claim · model-written summary
The screen found alleles of seven previously unknown genes.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- alleles of seven previously unidentified genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alleles of seven previously unidentified genes of unknown function”
e1“Arabidopsis thaliana”
Claim · model-written summary
npr1 mutations greatly reduced PR1 expression after infection.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- PsmES4326 infectione0
- Interactor
- PsmES4326e0pathogen
- Gene
- PR1e0
- Outcome
- expression of the PR1 genee0
- Direction
- greatly reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The npr1 mutations caused greatly reduced expression of the PR1 gene in response to PsmES4326 infection”
e1“Arabidopsis thaliana”
Claim · model-written summary
npr1 mutations had little effect on BGL2 and PR5 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- BGL2 and PR5e0
- Outcome
- expression of two other defense genese0
- Direction
- little effecte0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“had little effect on expression of two other defense genes, BGL2 and PR5”
e1“Arabidopsis thaliana”
Claim · model-written summary
Finding known defense mutants supports direct screening as a way to dissect resistance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- genetic dissection of the roles of various plant defense responses in disease resistancee0
- Comparator
- known defense responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutants isolated in this way include those affected in known defense responses supports the notion that this type of screening strategy allows genetic dissection of the roles of various plant defense responses in disease resistance”
e1“Arabidopsis thaliana”
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