Paper · 2024 · Journal of Agriculture and Food Research

Foliar urea applications to apple trees increase yeast assimilable nitrogen, amino acids, and flavor volatiles in fruit and hard cider

Brittany S. Cook; Michael G. Brown; Yanxin Lin; et al.

13
claims
25
verbatim quotes
9
citations
3
research areas
✓
Open access · cc-by-nc-nd

Abstract

Volatile compounds are a crucial component of hard apple (Malus ✕domestica Borkh.) cider aroma, flavor, and consequent marketability. Amino acids are used by Saccharomyces cerevisiae yeast in the production of volatiles and their precursors. This study hypothesized that foliar applications of nitrogen to apple trees would elevate the concentration of amino acids in the fruit, thus increasing the concentration of sensorially distinguishable aromatic volatiles in cider. In this experiment, urea foliar fertilizer was applied to ‘Ellis Bitter’ and ‘Harry Masters Jersey’ apple trees to create Control (0 applications), Low (3 weekly applications), and High (5 weekly applications) treatments. Total leaf nitrogen increased with the number of foliar urea applications, demonstrating plant uptake. The total fruit yield and fruit efficiency (total harvested fruit mass relative to trunk cross-sectional area) did not differ among treatments within the same cultivar. Similarly, fruit maturity, size, total phenolics, pH, and soluble solids concentration did not differ among treatments. Yeast assimilable nitrogen (YAN) and amino acids in the juice increased with the number of foliar urea applications, as did fermentation rates. Relative to the Control, the High treatment had 130% greater YAN in ‘Ellis Bitter’ and 145% in ‘Harry Masters Jersey’. Over 90% of the YAN in all juice samples was composed of primary amino nitrogen (PAN), and the majority of the PAN among all treatments was asparagine. Ester and fatty acid content in hard ciders increased with the number of urea applications; however, higher alcohols showed a mixed relationship with the number of applications in the cultivar ‘Ellis Bitter’. Aroma and flavor differences among treatments were discernible by triangle discrimination test panelists; however, the sensory differences were not easily identifiable with untrained panelists. This study demonstrated that late season foliar urea applications to apples trees can increase juice YAN and aromatic compounds in finished cider.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 13

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaf nitrogen increased with foliar urea applications.

Treatment
foliar urea applicationse0
Outcome
Total leaf nitrogene0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Total leaf nitrogen increased with the number of foliar urea applications, demonstrating plant uptake.”

✓ located verbatim in the source · abstract, characters 695–797 · show in text ↑
t0

“apple trees”

✓ located verbatim in the source · title, characters 28–39 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Fruit yield and fruit efficiency did not differ among treatments within cultivar.

Treatment
urea foliar fertilizere1
Outcome
total fruit yield and fruit efficiencye0
Direction
did not differe0
Qualifier
within the same cultivare0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The total fruit yield and fruit efficiency (total harvested fruit mass relative to trunk cross-sectional area) did not differ among treatments within the same cultivar.”

✓ located verbatim in the source · abstract, characters 798–966 · show in text ↑
t0

“apple trees”

✓ located verbatim in the source · title, characters 28–39 · show in text ↑
e1

“urea foliar fertilizer was applied”

✓ located verbatim in the source · abstract, characters 493–527 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Fruit maturity, size and total phenolics did not differ among treatments.

Treatment
urea foliar fertilizere1
Outcome
fruit maturity, size, total phenolicse0
Direction
did not differe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“fruit maturity, size, total phenolics, pH, and soluble solids concentration did not differ among treatments”

✓ located verbatim in the source · abstract, characters 978–1085 · show in text ↑
t0

“apple trees”

✓ located verbatim in the source · title, characters 28–39 · show in text ↑
e1

“urea foliar fertilizer was applied”

✓ located verbatim in the source · abstract, characters 493–527 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Fruit pH and soluble solids did not differ among treatments.

Treatment
urea foliar fertilizere1
Outcome
pH, and soluble solids concentratione0
Direction
did not differe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“fruit maturity, size, total phenolics, pH, and soluble solids concentration did not differ among treatments”

✓ located verbatim in the source · abstract, characters 978–1085 · show in text ↑
t0

“apple trees”

✓ located verbatim in the source · title, characters 28–39 · show in text ↑
e1

“urea foliar fertilizer was applied”

✓ located verbatim in the source · abstract, characters 493–527 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Juice YAN and amino acids increased with urea applications.

Treatment
foliar urea applicationse0
Outcome
Yeast assimilable nitrogen (YAN) and amino acidse0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Yeast assimilable nitrogen (YAN) and amino acids in the juice increased with the number of foliar urea applications, as did fermentation rates.”

✓ located verbatim in the source · abstract, characters 1087–1230 · show in text ↑
t0

“apple trees”

✓ located verbatim in the source · title, characters 28–39 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Fermentation rates increased with foliar urea applications.

Treatment
foliar urea applicationse0
Outcome
fermentation ratese0
Direction
increasede0

Not reportedTaxonComparator

Verbatim

e0

“Yeast assimilable nitrogen (YAN) and amino acids in the juice increased with the number of foliar urea applications, as did fermentation rates.”

✓ located verbatim in the source · abstract, characters 1087–1230 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

High treatment increased Ellis Bitter YAN by 130% relative to Control.

Material
‘Ellis Bitter’e0
Treatment
High treatmente0
Outcome
YANe0
Direction
greatere0
Value
130%e0
Comparator
Controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Relative to the Control, the High treatment had 130% greater YAN in ‘Ellis Bitter’ and 145% in ‘Harry Masters Jersey’.”

✓ located verbatim in the source · abstract, characters 1231–1349 · show in text ↑
t0

“apple trees”

✓ located verbatim in the source · title, characters 28–39 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

High treatment increased Harry Masters Jersey YAN by 145% relative to Control.

Material
‘Harry Masters Jersey’e0
Treatment
High treatmente0
Outcome
YANe0
Direction
greatere0
Value
145%e0
Comparator
Controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Relative to the Control, the High treatment had 130% greater YAN in ‘Ellis Bitter’ and 145% in ‘Harry Masters Jersey’.”

✓ located verbatim in the source · abstract, characters 1231–1349 · show in text ↑
t0

“apple trees”

✓ located verbatim in the source · title, characters 28–39 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Over 90% of juice YAN was PAN.

Outcome
primary amino nitrogen (PAN)e0
Value
Over 90%e0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“Over 90% of the YAN in all juice samples was composed of primary amino nitrogen (PAN)”

✓ located verbatim in the source · abstract, characters 1350–1435 · show in text ↑
t0

“apple trees”

✓ located verbatim in the source · title, characters 28–39 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Asparagine comprised the majority of PAN in all treatments.

Outcome
asparaginee0
Qualifier
the majority of the PANe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“the majority of the PAN among all treatments was asparagine”

✓ located verbatim in the source · abstract, characters 1441–1500 · show in text ↑
t0

“apple trees”

✓ located verbatim in the source · title, characters 28–39 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cider ester and fatty acid contents increased with urea applications.

Treatment
urea applicationse0
Outcome
Ester and fatty acid contente0
Direction
increasede0

Not reportedTaxonComparator

Verbatim

e0

“Ester and fatty acid content in hard ciders increased with the number of urea applications”

✓ located verbatim in the source · abstract, characters 1502–1592 · show in text ↑
Associationobservedoriginal resultmixedmeasured

Claim · model-written summary

Higher alcohols had a mixed relationship with applications in Ellis Bitter.

Material
‘Ellis Bitter’e0
Outcome
higher alcoholse0

Not reportedTaxonDirection

Verbatim

e0

“higher alcohols showed a mixed relationship with the number of applications in the cultivar ‘Ellis Bitter’”

✓ located verbatim in the source · abstract, characters 1603–1709 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Triangle panelists discerned treatment aroma and flavor differences, which untrained panelists could not easily identify.

Outcome
Aroma and flavor differencese0
Qualifier
the sensory differences were not easily identifiable with untrained panelistse0

Not reportedTaxon

Verbatim

e0

“Aroma and flavor differences among treatments were discernible by triangle discrimination test panelists; however, the sensory differences were not easily identifiable with untrained panelists.”

✓ located verbatim in the source · abstract, characters 1711–1904 · show in text ↑

nitrogen · yield · Malus domestica (apple)Results differ

69 papers: 52 positive, 17 negative or no effect

16 papers in the same direction as this paper · 52 in the opposite direction

  1. positive
  2. positive
  3. positive

65 papers more in this group

See all 69 papers →

nitrogen · quality: phytochemicals · Malus domestica (apple)Positive and negative results

10 papers: 4 positive, 6 negative or no effect

5 papers in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

fertilization · quality: phytochemicals · Malus domestica (apple)Positive and negative results

17 papers: 15 positive, 2 negative or no effect

1 paper in the same direction as this paper · 15 in the opposite direction

  1. positive
  2. positive
  3. positive

13 papers more in this group

See all 17 papers →

nitrogen · quality: sugar starch · Malus domestica (apple)Positive and negative results

18 papers: 4 positive, 14 negative or no effect

13 papers in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. positive

14 papers more in this group

See all 18 papers →

application · quality: phytochemicals · Malus domestica (apple)Positive and negative results

19 papers: 17 positive, 2 negative or no effect

1 paper in the same direction as this paper · 17 in the opposite direction

  1. positive
  2. positive
  3. positive

15 papers more in this group

See all 19 papers →

nitrogen · molecular: metabolites · Malus domestica (apple)Positive and negative results

19 papers: 9 positive, 10 negative or no effect

8 papers in the same direction as this paper · 10 in the opposite direction

  1. no effect
  2. negative
  3. negative

15 papers more in this group

See all 19 papers →

nitrogen · nutrient: content · Malus domestica (apple)Positive and negative results

38 papers: 26 positive, 12 negative or no effect

25 papers in the same direction as this paper · 12 in the opposite direction

  1. positive
  2. positive
  3. no effect

34 papers more in this group

See all 38 papers →

fertilization · quality: sugar starch · Malus domestica (apple)Positive and negative results

39 papers: 23 positive, 16 negative or no effect

15 papers in the same direction as this paper · 23 in the opposite direction

  1. no effect
  2. positive
  3. positive

35 papers more in this group

See all 39 papers →

8 of 13 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.